Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V8F66_RS10135 Genome accession   NZ_CP158484
Coordinates   2181028..2182800 (-) Length   590 a.a.
NCBI ID   WP_281662052.1    Uniprot ID   A0AAU7XSH1
Organism   Vreelandella sp. SM1641     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2176028..2187800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8F66_RS10105 (V8F66_10060) - 2176577..2176936 (+) 360 WP_281662048.1 hypothetical protein -
  V8F66_RS10110 (V8F66_10065) - 2176967..2177854 (-) 888 WP_350359617.1 DNA replication terminus site-binding protein -
  V8F66_RS10115 (V8F66_10070) yacG 2177894..2178127 (-) 234 WP_179915176.1 DNA gyrase inhibitor YacG -
  V8F66_RS10120 (V8F66_10075) coaE 2178161..2178763 (-) 603 WP_350359618.1 dephospho-CoA kinase -
  V8F66_RS10125 (V8F66_10080) pilD 2178873..2179718 (-) 846 WP_350359619.1 A24 family peptidase Machinery gene
  V8F66_RS10130 (V8F66_10085) pilC 2179795..2181024 (-) 1230 WP_350359620.1 type II secretion system F family protein Machinery gene
  V8F66_RS10135 (V8F66_10090) pilB 2181028..2182800 (-) 1773 WP_281662052.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V8F66_RS10140 (V8F66_10095) - 2183115..2183486 (+) 372 WP_281662053.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  V8F66_RS10145 (V8F66_10100) - 2183778..2184227 (+) 450 WP_281662054.1 anti-virulence regulator CigR family protein -
  V8F66_RS10150 (V8F66_10105) - 2184321..2185136 (+) 816 WP_281662055.1 inositol monophosphatase family protein -
  V8F66_RS10160 (V8F66_10115) - 2185513..2185725 (-) 213 WP_281662082.1 hypothetical protein -
  V8F66_RS10165 (V8F66_10120) - 2185664..2185864 (-) 201 WP_281662056.1 hypothetical protein -
  V8F66_RS10170 (V8F66_10125) - 2186143..2186346 (-) 204 WP_348646962.1 gamma-glutamylcyclotransferase family protein -
  V8F66_RS10175 (V8F66_10130) - 2186555..2187001 (-) 447 WP_281662057.1 YafY family protein -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64483.90 Da        Isoelectric Point: 6.2069

>NTDB_id=1011503 V8F66_RS10135 WP_281662052.1 2181028..2182800(-) (pilB) [Vreelandella sp. SM1641]
MSQATTESTLSYESTLSHAAARGGQRGIAQRLIKHGLLTETQAAAAASAAVENDISLLEHVVESGLVPPTAAAIAAGYEY
GLAVINLEAVRISSLPPVADYPVRILQSLNVVPLARHGHRLTVAVPYPATLTQLDELQFATGLSIEGILAPMDQLATTLS
SYLALTQSERGMLDEFNDLDDELSTLSVAEDSEVPLEEAPQNSDDGPIVKFVNKILLDAIRRGASDIHFEPYETQYRIRF
RIDGILIEVARPPLGMRNRVAARLKIMARLDISERRLPQDGAIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPSAAQL
GIEQLGFTDDQRHFYERALNQPQGMILVSGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLD
FASALRAFLRQDPDVVMVGEIRDLETADIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVTSFNIASAVSLIIAQRLTR
KLCPRCKEPVVIPHEALRKEGFSDEEIQQATIYKPVGCKHCTHGYKGRVGIYEVVPITEAMRQLIMSNANAMDIDQQARR
EGYPSLHQSGLLKVMRGITSLEEINRVSKE

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=1011503 V8F66_RS10135 WP_281662052.1 2181028..2182800(-) (pilB) [Vreelandella sp. SM1641]
ATGAGCCAAGCGACTACCGAATCAACATTAAGTTATGAGTCAACCCTAAGCCACGCCGCCGCACGCGGCGGGCAGCGCGG
TATTGCCCAGCGTTTAATCAAGCACGGCTTGCTAACCGAAACCCAGGCGGCGGCGGCCGCTAGCGCCGCCGTGGAAAATG
ATATTTCACTGTTAGAACACGTGGTTGAAAGCGGGCTTGTGCCCCCCACCGCCGCGGCGATTGCCGCAGGGTACGAGTAC
GGGCTAGCGGTTATCAATTTAGAAGCCGTTCGTATTAGCTCGCTGCCCCCTGTTGCTGACTACCCAGTGAGGATTCTGCA
AAGCTTAAATGTGGTGCCTTTGGCCCGTCATGGGCACCGTTTAACGGTGGCCGTGCCTTACCCCGCCACGCTTACCCAGC
TTGATGAGTTACAGTTTGCCACTGGGCTTAGCATTGAAGGGATATTAGCCCCGATGGACCAGCTAGCCACCACGCTGAGT
AGCTATTTAGCATTAACGCAAAGCGAACGTGGCATGCTGGACGAGTTTAATGACCTCGATGATGAGCTATCGACGCTGAG
CGTTGCCGAAGACAGCGAAGTGCCTTTGGAAGAGGCCCCCCAAAACAGTGATGATGGCCCCATCGTTAAGTTTGTTAATA
AGATTTTATTAGATGCGATTCGTCGTGGTGCCTCCGACATTCATTTCGAACCTTACGAAACGCAGTATCGTATCCGTTTT
CGTATTGACGGCATCTTAATTGAGGTCGCACGCCCTCCCTTAGGGATGCGTAACCGTGTTGCCGCCCGCCTGAAAATTAT
GGCGCGGCTGGATATTTCTGAGCGGCGCCTGCCCCAAGACGGAGCCATAAAACTTAAATTATCGCGCACGCGCTCGCTCG
ATTTTCGGGTCAACTCACTGCCCACGGTGTATGGCGAAAAATTGGTATTGCGTATTCTCGACCCCTCCGCGGCCCAGCTC
GGCATCGAGCAATTAGGCTTTACTGATGACCAACGTCACTTTTATGAACGTGCCCTCAACCAGCCCCAGGGAATGATACT
GGTGTCGGGCCCCACTGGCAGTGGCAAAACCGTGTCGCTGTATACCGGCATTAATATTCTCAATAAAGTGGAGCGCAATA
TTTGCACGGCGGAAGATCCGGTTGAGATTAAAGTAGCCGGTATTAATCAGGTCAACGTGTTACCTAAAATTGGGCTTGAT
TTTGCCAGTGCGCTACGGGCATTTCTGCGCCAAGACCCGGATGTGGTCATGGTGGGTGAAATACGGGATTTAGAAACCGC
TGACATTGCCGTTAAAGCCTCCCAGACAGGCCACTTGGTGCTTTCTACCGTGCACACTAACTCAGCCGCCGAAACGCTCA
CGCGGCTGGCGAACATGGGGGTAACGTCGTTTAACATCGCCAGTGCCGTTAGCCTGATTATTGCCCAACGCTTAACGCGT
AAACTCTGCCCACGCTGCAAGGAGCCGGTTGTTATCCCCCACGAGGCACTGCGCAAAGAGGGCTTTAGCGATGAAGAAAT
TCAACAGGCCACTATTTACAAGCCGGTAGGTTGTAAGCACTGCACGCATGGCTATAAAGGTCGCGTGGGTATTTATGAGG
TCGTTCCCATTACGGAAGCCATGCGCCAATTAATTATGAGCAACGCCAACGCGATGGATATCGATCAACAGGCGCGCCGC
GAAGGCTACCCCAGTTTGCACCAGAGCGGTTTATTAAAAGTCATGCGGGGGATTACCAGCCTTGAAGAAATTAACCGCGT
CAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-164)

Type II/IV secretion system protein (T2SSE)

(214-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.504

95.763

0.493
  pilB Acinetobacter baumannii D1279779

49.735

96.102

0.478
  pilB Vibrio campbellii strain DS40M4

50.36

94.237

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

49.556

95.424

0.473
  pilB Acinetobacter baylyi ADP1

49.293

95.932

0.473
  pilB Vibrio cholerae strain A1552

53.958

81.356

0.439
  pilF Neisseria gonorrhoeae MS11

50.417

81.356

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.813

90.678

0.361

Multiple sequence alignment