Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABNQ21_RS23995 Genome accession   NZ_CP158050
Coordinates   5214735..5216435 (+) Length   566 a.a.
NCBI ID   WP_226285871.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Y010     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5209735..5221435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNQ21_RS23970 (ABNQ21_23940) ampD 5209747..5210313 (-) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABNQ21_RS23975 (ABNQ21_23945) - 5210464..5212719 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  ABNQ21_RS23980 (ABNQ21_23950) nadC 5213005..5213853 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABNQ21_RS23990 (ABNQ21_23960) pilA 5214051..5214503 (-) 453 WP_023518088.1 pilin Machinery gene
  ABNQ21_RS23995 (ABNQ21_23965) pilB 5214735..5216435 (+) 1701 WP_226285871.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNQ21_RS24000 (ABNQ21_23970) pilC 5216439..5217659 (+) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  ABNQ21_RS24005 (ABNQ21_23975) pilD 5217663..5218535 (+) 873 WP_009875878.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABNQ21_RS24010 (ABNQ21_23980) coaE 5218532..5219143 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ABNQ21_RS24015 (ABNQ21_23985) yacG 5219140..5219340 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ABNQ21_RS24020 (ABNQ21_23990) - 5219377..5219586 (-) 210 WP_003094660.1 hypothetical protein -
  ABNQ21_RS24025 (ABNQ21_23995) - 5219692..5220381 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ABNQ21_RS24030 (ABNQ21_24000) - 5220378..5220848 (-) 471 WP_003103866.1 hypothetical protein -
  ABNQ21_RS24035 (ABNQ21_24005) - 5220845..5221270 (-) 426 WP_105248386.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62381.30 Da        Isoelectric Point: 5.7041

>NTDB_id=1009669 ABNQ21_RS23995 WP_226285871.1 5214735..5216435(+) (pilB) [Pseudomonas aeruginosa strain Y010]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPILWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1009669 ABNQ21_RS23995 WP_226285871.1 5214735..5216435(+) (pilB) [Pseudomonas aeruginosa strain Y010]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCAATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGATCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

49.823

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

40.072

97.88

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

93.11

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment