Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABL839_RS23240 Genome accession   NZ_CP157634
Coordinates   4994084..4995817 (+) Length   577 a.a.
NCBI ID   WP_019656541.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 110B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4989084..5000817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL839_RS23210 (ABL839_23235) proB 4989681..4990820 (-) 1140 WP_019656546.1 glutamate 5-kinase -
  ABL839_RS23215 (ABL839_23240) cgtA 4990910..4991986 (-) 1077 WP_019656545.1 Obg family GTPase CgtA -
  ABL839_RS23220 (ABL839_23245) rpmA 4992058..4992318 (-) 261 WP_019656544.1 50S ribosomal protein L27 -
  ABL839_RS23225 (ABL839_23250) rplU 4992340..4992651 (-) 312 WP_019656543.1 50S ribosomal protein L21 -
  ABL839_RS23230 (ABL839_23255) - 4992847..4993839 (+) 993 WP_019656542.1 polyprenyl synthetase family protein -
  ABL839_RS23240 (ABL839_23265) pilB 4994084..4995817 (+) 1734 WP_019656541.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABL839_RS23245 (ABL839_23270) pilC 4995844..4997073 (+) 1230 WP_019656540.1 type II secretion system F family protein Machinery gene
  ABL839_RS23250 (ABL839_23275) - 4997073..4998038 (+) 966 WP_019656539.1 prepilin peptidase -
  ABL839_RS23255 (ABL839_23280) coaE 4998046..4998657 (+) 612 WP_019656538.1 dephospho-CoA kinase -
  ABL839_RS23260 (ABL839_23285) - 4998727..5000220 (+) 1494 WP_019656537.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62558.79 Da        Isoelectric Point: 5.3302

>NTDB_id=1007893 ABL839_RS23240 WP_019656541.1 4994084..4995817(+) (pilB) [Variovorax paradoxus strain 110B]
MAAAELPVKEPSQIALPGLARALVSAGKLPAKAAEDIYQKSLTARTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDPQRLPKDLVDPKLCQAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDEVSAETANESNDQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHNYRVRFRIDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKLPADIPR
ETLLDAGFKEEELDGSWKPYRPVGCSACNSGYKGRVGIYQVMPISEAIQEIILRDGSALDIALQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1007893 ABL839_RS23240 WP_019656541.1 4994084..4995817(+) (pilB) [Variovorax paradoxus strain 110B]
ATGGCTGCAGCCGAACTTCCTGTTAAAGAACCTTCGCAAATAGCCCTGCCAGGGCTTGCGCGCGCCCTGGTTTCGGCCGG
CAAGCTTCCCGCCAAGGCTGCCGAGGACATCTATCAGAAGTCTCTTACTGCTCGCACAAGCTTCATCGCCGAGCTGACCG
GCTCCGGCGCCGTATCGGCAGCCGACCTCGCCCATACGCTTTCCTCCGCCTTCGGCGCCCCGCTGCTCGATCTGGACGCC
ATCGATCCGCAGCGCCTGCCGAAGGACCTGGTCGACCCGAAGCTGTGCCAGGCGTATCGCATCGTCGTCCTCAGCAAGCG
CAACAATCGCCTGATCGTCGCGACGGCCGATCCTTCAGACCAACAGGCGGCCGAGAAGATCAAGTTCGCCTCGCAGATGG
GCGTCGACTGGGTCATCGCCGAGTACGACAAGCTGTCGCGGATGATCGAGGCCGCCGCGGTCAGCGCGGCGGAGACCATC
AACAGCATCGTCGGCAGCGAGTTCGAGTTCGACGAGGTTTCCGCCGAGACTGCGAACGAGAGCAACGACCAGGCCATCGC
CGAAGTCGAAGACGCACCGGTCGTGCGTTTCCTGCACAAGATGCTGCTGGACGCCGTCAGCATGCGGGCATCGGACATCC
ACTTCGAACCGTACGAACACAACTACCGCGTGCGTTTCCGCATCGACGGCGAACTGCGCGAGGTGGCCAGCCCGCCGACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCCCGGCTCGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGATTTCCGCGTGAGCACGCTGCCCACGCTCTTCGGCGAGAAGA
TCGTCGTGCGTATTCTCGACCCGAGCAGCGCACGGCTGGGCATCGACGCCCTGGGCTACGACGCGGACGAGAAGGCGCGA
CTGTTGCATGCGATCGGCCGTCCCTATGGCATGGTGCTCGTCACCGGCCCCACGGGCTCGGGCAAGACGGTGTCGCTCTA
TACCTGCCTGAACCTGCTGAACCAGCCGGGCGTGAACATCGCGACGGCCGAAGACCCGTCCGAAATCAACCTGCCCGGCG
TCAACCAGGTGAACGTCAACGAGCGCGCGGGCCTGACCTTCGCGGCCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCTGCGCAGACCGGCCACCTCGTGCT
ATCCACGCTGCACACCAACGACGCGCCCACCACGCTCACGCGGATGCGCAACATGGGCATCGCACCGTTCAACATCGCGT
CGAGCGTGATCCTGATCACCGCCCAGCGCCTCGCGCGCCGCCTGTGCACGGTCTGCAAGCTCCCGGCCGACATTCCGCGT
GAAACGCTGCTGGACGCCGGCTTCAAGGAAGAGGAGCTGGACGGATCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCCGC
CTGCAACAGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGCGCGCTCGACATCGCCCTGCAGTCCGAGGCCGAGGGCGTGCGCTCCCTGCGGCAGTCGGGCCTGCGCAAG
GTCATGCAAGGTCTCACTTCGCTGGAAGAAGTGGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.982

97.4

0.536
  pilB Acinetobacter baylyi ADP1

54.448

97.4

0.53
  pilF Neisseria gonorrhoeae MS11

52.398

97.574

0.511
  pilB Legionella pneumophila strain ERS1305867

49.464

97.054

0.48
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio parahaemolyticus RIMD 2210633

45.683

96.36

0.44
  pilB Vibrio cholerae strain A1552

48.362

89.948

0.435
  pilF Thermus thermophilus HB27

38.354

98.96

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.223

93.068

0.374

Multiple sequence alignment