Detailed information NTG1007274

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ABLU27_RS02855
• Genome accession: NZ_CP157500
• Coordinates: 472031..472477 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis strain 2B-9
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 467031..477477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLU27_RS02820 (ABLU27_02820)	-	467148..467525 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
ABLU27_RS02825 (ABLU27_02825)	-	467730..468596 (+)	867	WP_058213299.1	RluA family pseudouridine synthase	-
ABLU27_RS02830 (ABLU27_02830)	-	468635..469444 (-)	810	WP_014570791.1	metal ABC transporter permease	-
ABLU27_RS02835 (ABLU27_02835)	-	469437..470174 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
ABLU27_RS02840 (ABLU27_02840)	-	470351..471193 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
ABLU27_RS02845 (ABLU27_02845)	-	471190..471627 (-)	438	WP_058213216.1	zinc-dependent MarR family transcriptional regulator	-
ABLU27_RS02850 (ABLU27_02850)	comGG	471708..471992 (-)	285	WP_406834849.1	competence type IV pilus minor pilin ComGG	Machinery gene
ABLU27_RS02855 (ABLU27_02855)	comGF	472031..472477 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
ABLU27_RS02860 (ABLU27_02860)	comGE	472440..472736 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
ABLU27_RS02865 (ABLU27_02865)	comGD	472708..473106 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
ABLU27_RS02870 (ABLU27_02870)	comGC	473099..473482 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
ABLU27_RS02875 (ABLU27_02875)	comGB	473496..474569 (-)	1074	WP_406835453.1	competence type IV pilus assembly protein ComGB	Machinery gene
ABLU27_RS02880 (ABLU27_02880)	comGA	474463..475401 (-)	939	WP_058212859.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=1007274 ABLU27_RS02855 WP_029344525.1 472031..472477(-) (comGF) [Lactococcus lactis strain 2B-9]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=1007274 ABLU27_RS02855 WP_029344525.1 472031..472477(-) (comGF) [Lactococcus lactis strain 2B-9]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCAATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385