Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QUD69_RS03115 Genome accession   NZ_AP027273
Coordinates   691836..693971 (-) Length   711 a.a.
NCBI ID   WP_269688125.1    Uniprot ID   -
Organism   Halopseudomonas aestusnigri strain MFKK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 686836..698971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD69_RS03100 (MFKK_06080) - 688586..690157 (-) 1572 WP_269688123.1 AAA family ATPase -
  QUD69_RS03105 (MFKK_06090) aroB 690154..691233 (-) 1080 WP_269688124.1 3-dehydroquinate synthase -
  QUD69_RS03110 (MFKK_06100) aroK 691321..691830 (-) 510 WP_228245559.1 shikimate kinase AroK -
  QUD69_RS03115 (MFKK_06110) pilQ 691836..693971 (-) 2136 WP_269688125.1 type IV pilus secretin PilQ Machinery gene
  QUD69_RS03120 (MFKK_06120) pilP 694028..694540 (-) 513 WP_228245571.1 type 4a pilus biogenesis lipoprotein PilP -
  QUD69_RS03125 (MFKK_06130) pilO 694555..695178 (-) 624 WP_088277117.1 type 4a pilus biogenesis protein PilO -
  QUD69_RS03130 (MFKK_06140) - 695175..695753 (-) 579 WP_233048949.1 PilN domain-containing protein -
  QUD69_RS03135 (MFKK_06150) pilM 695753..696817 (-) 1065 WP_228245562.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 711 a.a.        Molecular weight: 76449.34 Da        Isoelectric Point: 4.4330

>NTDB_id=100528 QUD69_RS03115 WP_269688125.1 691836..693971(-) (pilQ) [Halopseudomonas aestusnigri strain MFKK]
MSLTRIFPLAAVMSLLSPLALAADLQRMDVASLPGDRVELKLSFDEPVTGSPKGYTIDQPARIALDLPGVKNKLGERSRE
LGLGNARSVTVVEAGDRTRLIINLTSLAPYTTRAEGNDLYVMVGATAAAAPATTAAATPVATNNNWRTDNEPAVSSPVAS
GRAITSLDFQRGSDGSGNVVIDLSDPTIRVDVEEQGGRVKLNFPNTRLPDELRVQLDVKDFATPVNFVNASGSGDNASIV
IEPTGYYEHLVYQADNRLTVSFKPLTRAEVEQRRADAFAYTGEKLSLNFQDIEVRSVLQLIADFTDLNLVASDTVQGSIT
LRLQNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFASV
TAADADSQRGSLTVDERTNSIIALETQQKLDELRRIVTQLDIPVRQVMIEARIVEANVGFDKALGVRWGGAFNLGGNFSA
FGKDGQTDIVDAPDFLPPDTTSGGYYGVPERPANSPFVDMGVVGSTSGIGIGFITDNTILDLQLSAMESTGNGEVISQPK
VVTSDKETAKILKGSEIPYQEASSSGATTTSFADAVLSLEVTPQITPDNQVIMEVIVTKDEPDFSNQVNGVPTIRKNEVN
ANILVADGETIVIGGVFQSETSSAQEKVPFLGDLPLLGRMFRRDVTAESKTELLIFLTPRIINNGAITLAR

Nucleotide


Download         Length: 2136 bp        

>NTDB_id=100528 QUD69_RS03115 WP_269688125.1 691836..693971(-) (pilQ) [Halopseudomonas aestusnigri strain MFKK]
ATGTCTCTGACCAGAATTTTTCCGCTCGCAGCAGTAATGTCACTGCTGAGTCCGTTGGCCCTTGCGGCTGATCTGCAACG
TATGGATGTCGCTTCTCTGCCGGGGGATCGGGTAGAGCTCAAACTGAGCTTCGACGAGCCTGTCACCGGCTCGCCGAAGG
GTTACACCATCGACCAGCCGGCGCGGATCGCGCTGGACCTGCCAGGCGTCAAGAACAAGCTTGGTGAGCGCAGCCGCGAA
CTCGGTCTGGGTAATGCCCGGAGCGTGACCGTCGTTGAGGCCGGTGACCGTACCCGCCTGATCATCAATTTGACCAGCCT
GGCGCCCTACACCACCCGCGCCGAAGGCAATGACCTGTACGTGATGGTTGGTGCAACGGCAGCGGCAGCGCCGGCAACCA
CGGCCGCCGCCACTCCGGTGGCCACCAACAATAACTGGCGCACGGACAACGAGCCGGCGGTATCCAGTCCGGTCGCCAGT
GGTCGCGCTATCACCAGCCTGGACTTTCAGCGTGGCAGTGACGGCTCCGGTAATGTCGTCATTGACTTGAGTGATCCGAC
TATCCGGGTCGACGTGGAGGAGCAGGGCGGTCGCGTCAAGCTCAACTTCCCAAATACCCGCCTGCCTGACGAGCTGCGTG
TTCAGCTGGACGTCAAGGACTTCGCCACGCCGGTGAACTTCGTCAATGCCAGTGGCAGCGGCGACAATGCGTCTATCGTG
ATTGAGCCGACCGGCTATTACGAGCATCTGGTGTATCAGGCTGACAATCGCCTGACCGTCAGCTTCAAGCCGCTGACCCG
TGCCGAGGTGGAGCAGCGTCGTGCTGACGCGTTCGCCTATACCGGTGAGAAGCTGTCGCTGAACTTCCAGGATATCGAAG
TGCGCTCGGTGCTGCAGTTGATTGCCGACTTCACCGACCTGAACCTGGTAGCCAGCGATACCGTTCAGGGCAGCATCACG
CTGCGCCTGCAGAACGTGCCATGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGTCTGGATAAGCGTCAGGTGGG
TAATGTGTTGCTGGTAGCGCCGGCAGACGAGATCGCTGCACGCGAGCGTCAGGAGCTGGAGGCGCAGAAGCAGATTGCCG
AGTTGGCGCCGCTGCGTCGCGAGCTGATTCAGGTCAATTACGCCAAGGCGGCGGATATTGCCAAGCTGTTTGCCTCAGTG
ACGGCAGCTGACGCCGACTCTCAAAGGGGTTCGCTGACGGTTGATGAGCGTACCAACAGCATTATCGCGCTGGAAACCCA
GCAAAAGCTGGACGAGCTGCGCCGCATCGTGACTCAGCTGGACATTCCGGTTCGTCAGGTCATGATCGAAGCCCGGATTG
TTGAGGCCAACGTCGGCTTCGACAAGGCGCTGGGCGTGCGCTGGGGTGGCGCGTTCAATTTGGGCGGGAACTTCTCCGCT
TTCGGTAAGGACGGCCAGACCGACATCGTTGACGCGCCGGACTTTCTGCCCCCGGATACTACCTCTGGTGGCTATTACGG
TGTACCGGAGCGCCCGGCAAACTCTCCGTTCGTCGATATGGGCGTAGTGGGCTCCACCTCGGGGATCGGCATCGGCTTTA
TTACCGACAACACCATTCTGGATCTGCAACTGTCCGCGATGGAGAGCACCGGTAACGGTGAGGTGATTTCCCAGCCGAAA
GTGGTGACCTCCGACAAGGAAACGGCAAAGATCCTGAAAGGTTCGGAAATTCCGTATCAGGAAGCCAGCTCCAGCGGTGC
AACCACGACGTCCTTTGCCGATGCAGTGCTGTCGCTGGAAGTGACCCCCCAGATCACTCCGGACAACCAGGTGATCATGG
AAGTTATCGTGACCAAGGATGAGCCGGACTTCTCCAACCAGGTGAATGGGGTGCCGACCATTCGCAAGAATGAGGTCAAT
GCCAATATCCTGGTTGCCGACGGTGAAACTATCGTGATCGGTGGCGTATTCCAGAGCGAGACCTCCAGTGCTCAGGAAAA
GGTACCTTTCCTTGGTGATCTGCCGTTGCTCGGTCGGATGTTCCGTCGTGACGTAACGGCAGAGAGCAAGACCGAGCTGC
TGATTTTCCTGACGCCGCGGATCATCAACAACGGTGCCATCACGCTGGCCAGGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(308-354)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-446)

AMIN domain (AMIN)

(42-120)

Bacterial type II and III secretion system protein (Secretin)

(546-702)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.958

99.719

0.698
  comQ Acinetobacter baylyi ADP1

42.593

98.734

0.421
  pilQ Acinetobacter baumannii strain A118

39.84

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.84

100

0.419
  pilQ Legionella pneumophila strain ERS1305867

38.171

96.906

0.37
  pilQ Legionella pneumophila strain Lp02

38.171

96.906

0.37

Multiple sequence alignment