Detailed information NTG0100315

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: AACH30_RS05055
• Genome accession: NZ_AP027243
• Coordinates: 1024513..1025382 (-)
• Length: 289 a.a.
• NCBI ID: WP_265148857.1
• Uniprot ID: A0AA43PGF9
• Organism: Lactococcus garvieae strain L21-68
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1019513..1030382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACH30_RS05040 (LG21E68_09940)	-	1020491..1020712 (+)	222	WP_074749946.1	hypothetical protein	-
AACH30_RS05045 (LG21E68_09950)	trmFO	1020785..1022128 (-)	1344	WP_265148855.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
AACH30_RS05050 (LG21E68_09960)	topA	1022174..1024324 (-)	2151	WP_023888984.1	type I DNA topoisomerase	-
AACH30_RS05055 (LG21E68_09970)	dprA	1024513..1025382 (-)	870	WP_265148857.1	DNA-processing protein DprA	Machinery gene
AACH30_RS05060 (LG21E68_09980)	budA	1025469..1026167 (-)	699	WP_265148859.1	acetolactate decarboxylase	-
AACH30_RS05065 (LG21E68_09990)	-	1026309..1028033 (-)	1725	WP_004257235.1	ABC transporter permease/substrate binding protein	-
AACH30_RS05070 (LG21E68_10000)	-	1028026..1029228 (-)	1203	WP_014024780.1	glycine betaine/L-proline ABC transporter ATP-binding protein	-
AACH30_RS05075 (LG21E68_10010)	-	1029432..1030052 (-)	621	WP_004257227.1	GntR family transcriptional regulator	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32860.03 Da
• Isoelectric Point: 9.5626

>NTDB_id=100315 AACH30_RS05055 WP_265148857.1 1024513..1025382(-) (dprA) [Lactococcus garvieae strain L21-68]
MIRNFEILRLKKAGMSNLGILKLIDYQERHEAKLTLRQLARIAEVKAVPNFIESYKSQDVKRLREQYKTFPSFSILDDIY
PEWLKEMYNPPTLLFYQGNLKLLNFPKLGFVGSREMSKEAPKITYKLIEELKQSFVIVSGLARGVDTSSHVAAIKQQTPT
IAVIGNGLDISYPKENRKLQEYLATHELVLSEYLVGEPPLKFHFPERNRIIAGLSRGIVVVEAKQRSGSLITSRYALEGN
REVFAVPGDILNRNASGCNQLIQQGAAKLITHGQDILDEFYLYEGNFLP

Nucleotide

• Length: 870 bp

>NTDB_id=100315 AACH30_RS05055 WP_265148857.1 1024513..1025382(-) (dprA) [Lactococcus garvieae strain L21-68]
ATGATTAGAAATTTTGAAATATTGCGTTTGAAAAAAGCAGGAATGTCGAATCTAGGGATTTTGAAACTGATTGACTATCA
AGAGAGGCATGAAGCAAAACTTACTTTACGACAGTTAGCGAGGATTGCAGAAGTCAAAGCTGTTCCTAATTTTATTGAGA
GTTACAAGAGTCAGGATGTAAAACGACTTCGAGAGCAATATAAGACCTTCCCCTCCTTTTCCATCTTAGACGACATTTAT
CCTGAATGGCTGAAAGAAATGTATAATCCCCCGACCCTCCTTTTTTATCAAGGAAATCTTAAGTTGCTTAACTTCCCTAA
ACTCGGATTTGTCGGGAGCCGTGAAATGTCTAAGGAAGCACCTAAAATTACATACAAACTTATTGAGGAATTAAAACAAA
GCTTTGTTATAGTGAGCGGGTTAGCACGAGGTGTTGACACTTCTAGTCATGTAGCTGCTATAAAGCAACAAACACCAACC
ATTGCTGTCATTGGTAACGGTTTAGATATTAGTTATCCAAAAGAAAATCGTAAATTACAAGAGTATTTAGCAACTCATGA
ATTGGTTTTATCAGAATACCTTGTAGGAGAGCCGCCCCTTAAGTTTCATTTTCCAGAGAGAAATAGAATCATTGCGGGTC
TTTCTCGTGGAATTGTCGTTGTTGAAGCAAAGCAACGTAGCGGTAGCCTCATCACAAGTCGTTATGCCTTAGAGGGAAAT
AGAGAAGTATTTGCTGTACCTGGAGATATTTTGAACAGAAATGCCTCTGGCTGTAATCAATTAATCCAGCAAGGTGCAGC
CAAGCTTATTACTCATGGTCAAGATATTTTGGATGAATTTTATTTATATGAAGGTAATTTCCTTCCTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (74-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	63.958	97.924	0.626
dprA	Streptococcus mutans UA159	56.318	95.848	0.54
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	54.122	96.54	0.522
dprA/cilB/dalA	Streptococcus pneumoniae D39	54.122	96.54	0.522
dprA/cilB/dalA	Streptococcus pneumoniae R6	54.122	96.54	0.522
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	54.122	96.54	0.522
dprA/cilB/dalA	Streptococcus mitis SK321	54.122	96.54	0.522
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.405	96.54	0.516
dprA	Haemophilus influenzae Rd KW20	37.329	100	0.377