Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF86_RS13990 Genome accession   NZ_CP156780
Coordinates   2840981..2842690 (+) Length   569 a.a.
NCBI ID   WP_347454517.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7933     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2835981..2847690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF86_RS13945 (ABEF86_13945) nusA 2836569..2838053 (-) 1485 WP_347453476.1 transcription termination factor NusA -
  ABEF86_RS13950 (ABEF86_13950) rimP 2838089..2838613 (-) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF86_RS13980 (ABEF86_13980) secG 2839564..2839893 (-) 330 WP_034587516.1 preprotein translocase subunit SecG -
  ABEF86_RS13985 (ABEF86_13985) tpiA 2839907..2840698 (-) 792 WP_347454516.1 triose-phosphate isomerase -
  ABEF86_RS13990 (ABEF86_13990) pilB 2840981..2842690 (+) 1710 WP_347454517.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF86_RS13995 (ABEF86_13995) pilC 2842722..2843954 (+) 1233 WP_347454518.1 type II secretion system F family protein Machinery gene
  ABEF86_RS14000 (ABEF86_14000) pilD 2843969..2844826 (+) 858 WP_034587523.1 A24 family peptidase Machinery gene
  ABEF86_RS14005 (ABEF86_14005) coaE 2844831..2845430 (+) 600 WP_034587525.1 dephospho-CoA kinase -
  ABEF86_RS14010 (ABEF86_14010) - 2845455..2846375 (-) 921 WP_034587527.1 DMT family transporter -
  ABEF86_RS14015 (ABEF86_14015) rlmB 2846409..2847158 (-) 750 WP_347454519.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF86_RS14020 (ABEF86_14020) - 2847311..2847634 (-) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63347.64 Da        Isoelectric Point: 4.8664

>NTDB_id=1002732 ABEF86_RS13990 WP_347454517.1 2840981..2842690(+) (pilB) [Acinetobacter thermotolerans strain ANC 7933]
MTAIQGSLRFTGFIRRLVDERVISAPDMQSALAGAKQEKIDIVPYLIERHNISPTKIAETISIEFGEPLFDISAYDPALI
VREDFDEKIISKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEHNKLEKLIEQNFAEESTFDFDDDFD
LDVGVDASDSNTEEEDTNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKLADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002732 ABEF86_RS13990 WP_347454517.1 2840981..2842690(+) (pilB) [Acinetobacter thermotolerans strain ANC 7933]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAAGGGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTAGCTGGTGCAAAGCAAGAAAAAATCGACATCGTTCCATATCTGATTGAGCGCCATAATATTTCAC
CGACTAAAATTGCTGAAACCATTTCAATTGAGTTTGGCGAACCGTTGTTTGATATTAGTGCTTATGATCCTGCCCTGATT
GTTCGTGAAGACTTTGATGAAAAGATTATTAGTAAACATCGTATTCTACCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCGACCAGCAACCCGACCAATGTCGAAGCAATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACCATCATTG
TCGAGCATAATAAACTCGAAAAATTGATCGAACAGAATTTTGCTGAAGAAAGTACATTCGATTTTGATGATGATTTCGAC
CTTGATGTAGGTGTGGACGCGTCTGATTCAAATACTGAAGAGGAAGACACCAATACTGGTGATGAAGCACCAATTGTTAA
GTACATTAACAAGCTGTTAATTGATGCAATTCGGATGGGTGCATCAGACTTACACTTTGAACCGTATGAAAAAATATACC
GGGTACGTTACCGTATTGATGGTGTATTAAGACAAGTAGCAACACCACCGCTTCAGCTTGCAACCCGTCTATCTTCACGT
CTCAAAGTCATGTCTCAGATGGACATTTCGGAAAAACGTGTTCCTCAGGATGGCCGTATCAAGTTAAAACTGTCCAAAAA
CAAGGCCATTGATTTCCGTGTTAACTCATTACCCACGTTGTTTGGTGAAAAAATTGTATTGCGTATCCTGGATCCGTCCA
GTGCCATGTTAGGTATTGATGCATTAGGTTATGAACCCGATCAAAAAGCCCTGTTTATGGAAGCCCTGGACAAACCACAG
GGTATGCTGTTGATCACGGGTCCGACTGGTTCTGGTAAAACCGTCTCCTTATATACCGGCCTGAATATTCTGAATCGTGA
AGATACCAATATTTCTACCGCCGAAGACCCAGTGGAAATCAACTTACAGGGTATTAACCAGGTCAACGTAAACCCTAAAG
TCGGTTTAACCTTTGCTGCAGCACTGAAATCATTCCTGCGTCAAGATCCGGATATCGTCATGGTCGGTGAGATCCGAGAC
TTGGAAACAGCTGAAATTGCGGTTAAAGCAGCGCAGACTGGTCACATGGTGATGTCGACACTACACACCAACAGTGCTCC
AGAAACACTGACCCGTTTACGCAATATGGGTGTACCATCATTTAACATTGCCACCTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCGCGTCGTCTGTGTTCACAATGCAAAAAACTGGCTGATATTCCAAAGCAGAGTCTGCTGGAAATGGGCTTTACC
GAAGAAGATTTACAGAACCCAGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAAGGCCG
TGTCGGTATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.316

100

0.765
  pilB Legionella pneumophila strain ERS1305867

51.499

99.649

0.513
  pilB Vibrio cholerae strain A1552

49.381

99.297

0.49
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.909

96.485

0.482
  pilB Vibrio campbellii strain DS40M4

48.44

95.782

0.464
  pilF Thermus thermophilus HB27

37.341

96.485

0.36

Multiple sequence alignment