Detailed information NTG1002734

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ABEF86_RS14000
• Genome accession: NZ_CP156780
• Coordinates: 2843969..2844826 (+)
• Length: 285 a.a.
• NCBI ID: WP_034587523.1
• Uniprot ID: A0A0A3W448
• Organism: Acinetobacter thermotolerans strain ANC 7933
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2838969..2849826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEF86_RS13980 (ABEF86_13980)	secG	2839564..2839893 (-)	330	WP_034587516.1	preprotein translocase subunit SecG	-
ABEF86_RS13985 (ABEF86_13985)	tpiA	2839907..2840698 (-)	792	WP_347454516.1	triose-phosphate isomerase	-
ABEF86_RS13990 (ABEF86_13990)	pilB	2840981..2842690 (+)	1710	WP_347454517.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABEF86_RS13995 (ABEF86_13995)	pilC	2842722..2843954 (+)	1233	WP_347454518.1	type II secretion system F family protein	Machinery gene
ABEF86_RS14000 (ABEF86_14000)	pilD	2843969..2844826 (+)	858	WP_034587523.1	A24 family peptidase	Machinery gene
ABEF86_RS14005 (ABEF86_14005)	coaE	2844831..2845430 (+)	600	WP_034587525.1	dephospho-CoA kinase	-
ABEF86_RS14010 (ABEF86_14010)	-	2845455..2846375 (-)	921	WP_034587527.1	DMT family transporter	-
ABEF86_RS14015 (ABEF86_14015)	rlmB	2846409..2847158 (-)	750	WP_347454519.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
ABEF86_RS14020 (ABEF86_14020)	-	2847311..2847634 (-)	324	WP_034587531.1	pyrimidine/purine nucleoside phosphorylase	-
ABEF86_RS14025 (ABEF86_14025)	recN	2848048..2849709 (+)	1662	WP_347453466.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 285 a.a.
• Molecular weight: 31766.05 Da
• Isoelectric Point: 8.0605

>NTDB_id=1002734 ABEF86_RS14000 WP_034587523.1 2843969..2844826(+) (pilD) [Acinetobacter thermotolerans strain ANC 7933]
MNSLIDFLIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQLFLHPDQPIIDESKITLSKPASTCPKCQTP
IRWYQNIPVISWLALRGKCGSCHTQISARYPFIEILTAVCALIILAVFGPTVQMLFGLLLTYVLIALTFIDFDTQLLPDR
FTLPLAALGLGINSYSIYTSPSSAIWGYIIGFLCLWLVYYLFKIITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKAYLG

Nucleotide

• Length: 858 bp

>NTDB_id=1002734 ABEF86_RS14000 WP_034587523.1 2843969..2844826(+) (pilD) [Acinetobacter thermotolerans strain ANC 7933]
TTGAATAGTTTAATTGATTTTTTAATTCAAAACCCGACTGCGCTCTATATCGCAGTTGGGCTTTTTAGCCTCTGTATCGG
CAGTTTTCTGAATGTAGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGAACCGATTGCCAGCTGTTTTTAC
ATCCAGATCAGCCGATCATTGATGAAAGCAAAATCACTCTAAGCAAACCAGCATCCACTTGTCCGAAATGCCAGACACCT
ATCCGCTGGTACCAGAATATTCCTGTGATCAGCTGGCTAGCTTTACGCGGAAAATGTGGCTCCTGCCATACTCAGATAAG
TGCCCGTTATCCGTTTATTGAGATTCTGACAGCAGTATGTGCACTCATCATTTTGGCAGTCTTTGGCCCAACCGTACAGA
TGCTGTTTGGACTACTACTGACTTATGTGTTGATTGCATTAACCTTTATCGACTTCGATACACAGCTCCTACCAGATCGC
TTTACCCTGCCACTCGCTGCACTGGGTCTAGGGATTAATAGCTACAGTATTTATACCTCACCCTCTTCAGCGATCTGGGG
GTATATTATTGGTTTTCTGTGCCTTTGGCTCGTGTATTACCTGTTCAAGATCATTACAGGGAAAGAAGGTATGGGCTATG
GTGACTTTAAGCTCTTGGCTGCGCTAGGTGCTTGGATGGGTCCACTGATGCTGCCGCTGATCGTATTGCTATCCTCACTG
GTCGGTGCCATTATCGGCATCATCCTATTAAAAGTCCGCAAGGAAAATCAGCCTTTTGCTTTTGGTCCCTATATCGCCAT
TGCCGGCTGGATTGCTTTCCTTTGGGGTGAACAGATCATGAAAGCATATTTGGGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

• Type IV leader peptidase family (Peptidase_A24) (139-247)

3D structure

• AlphaFold DB: A0A0A3W448

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	80	100	0.8
pilD	Acinetobacter nosocomialis M2	79.649	100	0.796
pilD	Vibrio cholerae strain A1552	56.18	93.684	0.526
pilD	Vibrio campbellii strain DS40M4	53.184	93.684	0.498
pilD	Neisseria gonorrhoeae MS11	46.953	97.895	0.46