Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEF83_RS13895 Genome accession   NZ_CP156768
Coordinates   2830633..2832342 (+) Length   569 a.a.
NCBI ID   WP_347454517.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7974     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825633..2837342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF83_RS13850 (ABEF83_13850) nusA 2826221..2827705 (-) 1485 WP_347453476.1 transcription termination factor NusA -
  ABEF83_RS13855 (ABEF83_13855) rimP 2827741..2828265 (-) 525 WP_034587514.1 ribosome maturation factor RimP -
  ABEF83_RS13885 (ABEF83_13885) secG 2829216..2829545 (-) 330 WP_034587516.1 preprotein translocase subunit SecG -
  ABEF83_RS13890 (ABEF83_13890) tpiA 2829559..2830350 (-) 792 WP_347454516.1 triose-phosphate isomerase -
  ABEF83_RS13895 (ABEF83_13895) pilB 2830633..2832342 (+) 1710 WP_347454517.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEF83_RS13900 (ABEF83_13900) pilC 2832374..2833606 (+) 1233 WP_347454518.1 type II secretion system F family protein Machinery gene
  ABEF83_RS13905 (ABEF83_13905) pilD 2833621..2834478 (+) 858 WP_034587523.1 A24 family peptidase Machinery gene
  ABEF83_RS13910 (ABEF83_13910) coaE 2834483..2835082 (+) 600 WP_034587525.1 dephospho-CoA kinase -
  ABEF83_RS13915 (ABEF83_13915) - 2835107..2836027 (-) 921 WP_034587527.1 DMT family transporter -
  ABEF83_RS13920 (ABEF83_13920) rlmB 2836061..2836810 (-) 750 WP_347454519.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEF83_RS13925 (ABEF83_13925) - 2836963..2837286 (-) 324 WP_034587531.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63347.64 Da        Isoelectric Point: 4.8664

>NTDB_id=1002567 ABEF83_RS13895 WP_347454517.1 2830633..2832342(+) (pilB) [Acinetobacter thermotolerans strain ANC 7974]
MTAIQGSLRFTGFIRRLVDERVISAPDMQSALAGAKQEKIDIVPYLIERHNISPTKIAETISIEFGEPLFDISAYDPALI
VREDFDEKIISKHRILPIFKSANILFVATSNPTNVEAIDAIRFATKLNIETIIVEHNKLEKLIEQNFAEESTFDFDDDFD
LDVGVDASDSNTEEEDTNTGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRIDGVLRQVATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKLADIPKQSLLEMGFT
EEDLQNPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1002567 ABEF83_RS13895 WP_347454517.1 2830633..2832342(+) (pilB) [Acinetobacter thermotolerans strain ANC 7974]
ATGACAGCTATTCAGGGATCTTTACGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAAGGGTTATCTCTGCACCAGA
TATGCAGAGTGCTTTAGCTGGTGCAAAGCAAGAAAAAATCGACATCGTTCCATATCTGATTGAGCGCCATAATATTTCAC
CGACTAAAATTGCTGAAACCATTTCAATTGAGTTTGGCGAACCGTTGTTTGATATTAGTGCTTATGATCCTGCCCTGATT
GTTCGTGAAGACTTTGATGAAAAGATTATTAGTAAACATCGTATTCTACCGATCTTTAAAAGTGCCAATATCCTGTTTGT
TGCGACCAGCAACCCGACCAATGTCGAAGCAATTGATGCGATCCGTTTTGCCACCAAGCTCAATATTGAAACCATCATTG
TCGAGCATAATAAACTCGAAAAATTGATCGAACAGAATTTTGCTGAAGAAAGTACATTCGATTTTGATGATGATTTCGAC
CTTGATGTAGGTGTGGACGCGTCTGATTCAAATACTGAAGAGGAAGACACCAATACTGGTGATGAAGCACCAATTGTTAA
GTACATTAACAAGCTGTTAATTGATGCAATTCGGATGGGTGCATCAGACTTACACTTTGAACCGTATGAAAAAATATACC
GGGTACGTTACCGTATTGATGGTGTATTAAGACAAGTAGCAACACCACCGCTTCAGCTTGCAACCCGTCTATCTTCACGT
CTCAAAGTCATGTCTCAGATGGACATTTCGGAAAAACGTGTTCCTCAGGATGGCCGTATCAAGTTAAAACTGTCCAAAAA
CAAGGCCATTGATTTCCGTGTTAACTCATTACCCACGTTGTTTGGTGAAAAAATTGTATTGCGTATCCTGGATCCGTCCA
GTGCCATGTTAGGTATTGATGCATTAGGTTATGAACCCGATCAAAAAGCCCTGTTTATGGAAGCCCTGGACAAACCACAG
GGTATGCTGTTGATCACGGGTCCGACTGGTTCTGGTAAAACCGTCTCCTTATATACCGGCCTGAATATTCTGAATCGTGA
AGATACCAATATTTCTACCGCCGAAGACCCAGTGGAAATCAACTTACAGGGTATTAACCAGGTCAACGTAAACCCTAAAG
TCGGTTTAACCTTTGCTGCAGCACTGAAATCATTCCTGCGTCAAGATCCGGATATCGTCATGGTCGGTGAGATCCGAGAC
TTGGAAACAGCTGAAATTGCGGTTAAAGCAGCGCAGACTGGTCACATGGTGATGTCGACACTACACACCAACAGTGCTCC
AGAAACACTGACCCGTTTACGCAATATGGGTGTACCATCATTTAACATTGCCACCTCAGTCAATCTGGTGATTGCCCAGC
GTCTGGCGCGTCGTCTGTGTTCACAATGCAAAAAACTGGCTGATATTCCAAAGCAGAGTCTGCTGGAAATGGGCTTTACC
GAAGAAGATTTACAGAACCCAGAATTCCAGATTTATGAACCTGTAGGGTGCAATGAATGCCGTGAAGGTTATAAAGGCCG
TGTCGGTATTTATGAAGTCATGAAGGTGACACCGGAAATTTCACGCATTATTATGGAAGATGGCAACGCCATTCAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.316

100

0.765
  pilB Legionella pneumophila strain ERS1305867

51.499

99.649

0.513
  pilB Vibrio cholerae strain A1552

49.381

99.297

0.49
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.909

96.485

0.482
  pilB Vibrio campbellii strain DS40M4

48.44

95.782

0.464
  pilF Thermus thermophilus HB27

37.341

96.485

0.36

Multiple sequence alignment