Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGD   Type   Machinery gene
Locus tag   AACH63_RS00795 Genome accession   NZ_AP027240
Coordinates   156064..156480 (+) Length   138 a.a.
NCBI ID   WP_096368799.1    Uniprot ID   -
Organism   Lactococcus formosensis strain L21-20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 151064..161480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH63_RS00770 (LG21E20_01480) - 151711..153372 (+) 1662 WP_096369379.1 S-layer homology domain-containing protein -
  AACH63_RS00775 (LG21E20_01490) rpsU 153501..153677 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH63_RS00780 (LG21E20_01500) comGA 153875..154807 (+) 933 WP_286210749.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH63_RS00785 (LG21E20_01510) comGB 154746..155765 (+) 1020 WP_269457350.1 competence type IV pilus assembly protein ComGB Machinery gene
  AACH63_RS00790 (LG21E20_01520) comYC 155784..156089 (+) 306 WP_096368800.1 competence type IV pilus major pilin ComGC Machinery gene
  AACH63_RS00795 comGD 156064..156480 (+) 417 WP_096368799.1 competence type IV pilus minor pilin ComGD Machinery gene
  AACH63_RS00800 comGE 156512..156745 (+) 234 WP_269457349.1 competence type IV pilus minor pilin ComGE -
  AACH63_RS00805 (LG21E20_01540) comGF 156726..157166 (+) 441 WP_096368797.1 competence type IV pilus minor pilin ComGF -
  AACH63_RS00810 (LG21E20_01550) - 157401..158246 (+) 846 WP_096368796.1 zinc ABC transporter substrate-binding protein -
  AACH63_RS00815 (LG21E20_01560) - 158333..159202 (-) 870 WP_096368795.1 RluA family pseudouridine synthase -

Sequence


Protein


Download         Length: 138 a.a.        Molecular weight: 15899.56 Da        Isoelectric Point: 10.3023

>NTDB_id=100244 AACH63_RS00795 WP_096368799.1 156064..156480(+) (comGD) [Lactococcus formosensis strain L21-20]
MQNVISRIKALTLLESLLVLFLCSFVLFLFSSSVKQSVRIARSEIFILQFERLYKDTQRMAGLKGQRQTLSARNGTLYSQ
EEMLKVPEEAEINDFSITFDQKSGNSSLQKITIFLPYQKKTISYQLEIGSGKYKKRIT

Nucleotide


Download         Length: 417 bp        

>NTDB_id=100244 AACH63_RS00795 WP_096368799.1 156064..156480(+) (comGD) [Lactococcus formosensis strain L21-20]
ATGCAAAACGTAATATCCCGAATTAAAGCTTTAACTTTACTGGAGAGTTTACTCGTTCTTTTCCTATGCTCTTTTGTTCT
TTTTCTATTTTCAAGTTCGGTGAAACAAAGTGTGAGGATAGCACGTTCAGAGATTTTTATTTTACAATTTGAACGTCTGT
ATAAGGATACCCAGCGCATGGCTGGACTAAAGGGGCAGCGACAGACATTGAGTGCGCGTAATGGCACACTATATAGCCAA
GAAGAAATGTTGAAAGTCCCAGAGGAGGCTGAAATAAATGATTTCTCTATTACTTTTGATCAAAAATCAGGCAATTCGAG
CCTACAAAAAATCACAATATTTCTTCCCTATCAGAAAAAAACGATCTCTTATCAATTGGAAATTGGAAGTGGAAAATATA
AGAAGAGAATCACTTGA

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Lactococcus lactis subsp. cremoris KW2

50

100

0.5

  comYD Streptococcus mutans UA140

40.909

95.652

0.391

  comYD Streptococcus mutans UA159

40.909

95.652

0.391

  comGD/cglD Streptococcus mitis SK321

40.31

93.478

0.377

  comGD/cglD Streptococcus pneumoniae TIGR4

39.535

93.478

0.37

  comGD/cglD Streptococcus mitis NCTC 12261

38.462

94.203

0.362


Multiple sequence alignment