Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   AACH63_RS00785 Genome accession   NZ_AP027240
Coordinates   154746..155765 (+) Length   339 a.a.
NCBI ID   WP_269457350.1    Uniprot ID   -
Organism   Lactococcus formosensis strain L21-20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 149746..160765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH63_RS00765 (LG21E20_01470) guaB 149874..151355 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  AACH63_RS00770 (LG21E20_01480) - 151711..153372 (+) 1662 WP_096369379.1 S-layer homology domain-containing protein -
  AACH63_RS00775 (LG21E20_01490) rpsU 153501..153677 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  AACH63_RS00780 (LG21E20_01500) comGA 153875..154807 (+) 933 WP_286210749.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH63_RS00785 (LG21E20_01510) comGB 154746..155765 (+) 1020 WP_269457350.1 competence type IV pilus assembly protein ComGB Machinery gene
  AACH63_RS00790 (LG21E20_01520) comYC 155784..156089 (+) 306 WP_096368800.1 competence type IV pilus major pilin ComGC Machinery gene
  AACH63_RS00795 comGD 156064..156480 (+) 417 WP_096368799.1 competence type IV pilus minor pilin ComGD Machinery gene
  AACH63_RS00800 comGE 156512..156745 (+) 234 WP_269457349.1 competence type IV pilus minor pilin ComGE -
  AACH63_RS00805 (LG21E20_01540) comGF 156726..157166 (+) 441 WP_096368797.1 competence type IV pilus minor pilin ComGF -
  AACH63_RS00810 (LG21E20_01550) - 157401..158246 (+) 846 WP_096368796.1 zinc ABC transporter substrate-binding protein -
  AACH63_RS00815 (LG21E20_01560) - 158333..159202 (-) 870 WP_096368795.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39406.06 Da        Isoelectric Point: 9.2554

>NTDB_id=100242 AACH63_RS00785 WP_269457350.1 154746..155765(+) (comGB) [Lactococcus formosensis strain L21-20]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFYEDI
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGVEFLQILDSGQKFEDALRFYPIFTKELALIVEYGELKAKLGQELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=100242 AACH63_RS00785 WP_269457350.1 154746..155765(+) (comGB) [Lactococcus formosensis strain L21-20]
ATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATTCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGATTAACGGAGGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTTATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTGGGGCTGATTGAAGAAAAACTGATACGCGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GCTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGCTATACAAAATCTTCCGACCATC
TTTTTAGGGTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTTAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGACTTACGTCAGATTTGTTTCATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCCCTTCGATTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGCTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.254

99.705

0.531
  comGB/cglB Streptococcus mitis NCTC 12261

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae D39

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae R6

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae TIGR4

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae Rx1

51.94

98.82

0.513
  comYB Streptococcus gordonii str. Challis substr. CH1

51.497

98.525

0.507
  comYB Streptococcus mutans UA140

50.794

92.92

0.472
  comYB Streptococcus mutans UA159

50.476

92.92

0.469

Multiple sequence alignment