Detailed information NTG0100225

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: AACH29_RS05310
• Genome accession: NZ_AP027239
• Coordinates: 1077603..1078472 (+)
• Length: 289 a.a.
• NCBI ID: WP_014024779.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain L21-12
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1072603..1083472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACH29_RS05290 (LG21E12_10480)	-	1072932..1073552 (+)	621	WP_014024781.1	GntR family transcriptional regulator	-
AACH29_RS05295 (LG21E12_10490)	-	1073757..1074959 (+)	1203	WP_014024780.1	glycine betaine/L-proline ABC transporter ATP-binding protein	-
AACH29_RS05300 (LG21E12_10500)	-	1074952..1076676 (+)	1725	WP_004257235.1	ABC transporter permease/substrate binding protein	-
AACH29_RS05305 (LG21E12_10510)	budA	1076818..1077516 (+)	699	WP_004257238.1	acetolactate decarboxylase	-
AACH29_RS05310 (LG21E12_10520)	dprA	1077603..1078472 (+)	870	WP_014024779.1	DNA-processing protein DprA	Machinery gene
AACH29_RS05315 (LG21E12_10530)	topA	1078661..1080811 (+)	2151	WP_014024778.1	type I DNA topoisomerase	-
AACH29_RS05320 (LG21E12_10540)	trmFO	1080856..1082199 (+)	1344	WP_014024777.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
AACH29_RS05325 (LG21E12_10550)	-	1082272..1082493 (-)	222	WP_014024776.1	hypothetical protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32873.03 Da
• Isoelectric Point: 9.5626

>NTDB_id=100225 AACH29_RS05310 WP_014024779.1 1077603..1078472(+) (dprA) [Lactococcus garvieae strain L21-12]
MIRNFEILRLKKAGMSNLGILKLIDYQERHEAKLTLRQLARIAEVKAVPNFIESYKSQDVKRLREQYKTFPSFSILDDIY
PEWLKEMYNPPTLLFYQGNLKLLNFPKLGFVGSREMSKEAPKITYKLIEELKQNFVIVSGLARGVDTSSHVAAIKQQTPT
IAVIGNGLDISYPKENRKLQEYLATHELVLSEYLVGEPPLKFHFPERNRIIAGLSRGIVVVEAKQRSGSLISSRYALEGN
REVFAVPGDILNRNASGCNQLIQQGAAKLITHGQDILDEFYLYEGNFLP

Nucleotide

• Length: 870 bp

>NTDB_id=100225 AACH29_RS05310 WP_014024779.1 1077603..1078472(+) (dprA) [Lactococcus garvieae strain L21-12]
ATGATTAGAAATTTTGAAATATTGCGTTTGAAAAAAGCAGGAATGTCGAATCTAGGGATTTTGAAACTGATTGACTATCA
AGAGAGGCATGAAGCAAAACTTACTTTACGACAGTTAGCGAGGATCGCAGAAGTCAAAGCTGTCCCTAATTTTATTGAGA
GTTACAAGAGTCAGGATGTGAAACGACTTCGAGAGCAATATAAGACCTTCCCCTCCTTTTCCATCTTAGACGACATTTAT
CCTGAATGGCTGAAAGAAATGTATAATCCCCCGACTCTCCTTTTTTATCAAGGAAATCTTAAGTTGCTTAATTTCCCTAA
ACTCGGATTTGTCGGGAGCCGTGAAATGTCTAAGGAAGCACCTAAAATTACATACAAACTTATTGAGGAATTAAAACAAA
ACTTTGTTATAGTGAGCGGGTTAGCACGCGGTGTTGACACTTCTAGTCATGTAGCTGCTATAAAGCAACAAACACCAACC
ATTGCTGTCATTGGTAACGGTTTAGATATTAGTTATCCAAAAGAAAATCGTAAATTACAAGAGTATTTAGCCACTCATGA
ATTGGTTTTATCAGAATACCTTGTAGGAGAGCCTCCCCTTAAGTTTCATTTTCCAGAGAGAAATAGAATCATTGCGGGTC
TTTCTCGTGGAATTGTCGTTGTTGAAGCAAAGCAACGTAGCGGTAGCCTCATCTCAAGTCGTTATGCCTTAGAGGGAAAT
AGAGAAGTATTTGCTGTACCTGGAGATATTTTGAACAGAAATGCCTCTGGCTGTAATCAATTAATCCAGCAAGGTGCAGC
CAAGCTTATCACTCATGGTCAAGATATTTTGGATGAATTTTATTTATATGAGGGTAATTTCCTTCCTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (74-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	63.251	97.924	0.619
dprA	Streptococcus mutans UA159	55.957	95.848	0.536
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.763	96.54	0.519
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.763	96.54	0.519
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.763	96.54	0.519
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.763	96.54	0.519
dprA/cilB/dalA	Streptococcus mitis SK321	53.763	96.54	0.519
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.047	96.54	0.512
dprA	Haemophilus influenzae Rd KW20	36.986	100	0.374