Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFV80_RS14680 Genome accession   NZ_CP156659
Coordinates   3282085..3283815 (+) Length   576 a.a.
NCBI ID   WP_347485046.1    Uniprot ID   -
Organism   Vandammella animalimorsus strain SK20231019-0001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3277085..3288815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV80_RS14660 (ABFV80_002932) corA 3278083..3279066 (-) 984 WP_095550010.1 magnesium/cobalt transporter CorA -
  ABFV80_RS14665 (ABFV80_002933) - 3279326..3279808 (-) 483 WP_343292824.1 uracil-DNA glycosylase family protein -
  ABFV80_RS14670 (ABFV80_002934) rimI 3280353..3280877 (-) 525 WP_238944884.1 ribosomal protein S18-alanine N-acetyltransferase -
  ABFV80_RS14675 (ABFV80_002935) - 3280877..3281788 (-) 912 WP_347485045.1 M23 family metallopeptidase -
  ABFV80_RS14680 (ABFV80_002936) pilB 3282085..3283815 (+) 1731 WP_347485046.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFV80_RS14685 (ABFV80_002937) pilC 3283846..3285087 (+) 1242 WP_347485047.1 type II secretion system F family protein Machinery gene
  ABFV80_RS14690 (ABFV80_002938) - 3285087..3285965 (+) 879 WP_095542925.1 A24 family peptidase -
  ABFV80_RS14695 (ABFV80_002939) - 3286186..3287175 (-) 990 WP_347485048.1 tripartite tricarboxylate transporter substrate binding protein -
  ABFV80_RS14700 (ABFV80_002940) - 3287285..3288508 (-) 1224 WP_347485050.1 CaiB/BaiF CoA-transferase family protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62906.78 Da        Isoelectric Point: 4.7661

>NTDB_id=1001899 ABFV80_RS14680 WP_347485046.1 3282085..3283815(+) (pilB) [Vandammella animalimorsus strain SK20231019-0001]
MATEDLTKKATAELVVPSVARAFLVAKQLTQEQVDATLQRARNEGRDFLSALVEGNVLSATQAAKTISDVFAVPYIDLGA
VDAMRLPADLLDLKLCLSYKVVVLGKRQSRLIVASADPSNRDTAEKLKFATQASIEWLVAAHDKISALLDELAKANALDG
QSSDFQFDELDGTVEEVSDDDAGDASSDADDAPVVRFLQKMIIDAYRLGASDLHFEPYEFNYRVRFRIDGELREYAAPPV
SIKERLASRIKVISRLDIAEKRIPQDGRTKMRIGGKSIDFRVSTLPTLFGEKIVIRILDSSNAKIGIDALGYEPIEKERL
LTAIERPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLRNMGIAPFNIASSVILITAQRLARRLCSQCREETDIPVD
ALIDAGFNYDDLDGSWVPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILNDGSAIEIQEQAEKEGVRTLRQSGLQKV
KLGVTSLEEVLAVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1001899 ABFV80_RS14680 WP_347485046.1 3282085..3283815(+) (pilB) [Vandammella animalimorsus strain SK20231019-0001]
ATGGCCACTGAAGACCTTACCAAAAAAGCCACTGCCGAACTGGTTGTGCCCAGCGTTGCCAGGGCATTTCTGGTGGCCAA
GCAGCTGACGCAGGAGCAGGTGGATGCCACGCTGCAGCGCGCCCGCAACGAAGGGCGGGACTTTCTCAGTGCGCTGGTCG
AAGGCAACGTTCTGTCGGCCACCCAGGCGGCAAAAACCATTTCGGATGTTTTTGCTGTGCCTTACATCGACCTGGGCGCC
GTCGATGCCATGCGCCTGCCGGCCGATCTGCTGGATTTGAAGCTGTGCCTGTCCTACAAAGTGGTTGTGCTGGGCAAGCG
CCAGAGCCGGCTGATCGTCGCCAGCGCCGACCCGAGCAACCGAGATACGGCGGAGAAGCTGAAATTTGCCACCCAGGCCT
CCATCGAGTGGCTGGTGGCGGCGCACGACAAGATCTCAGCCTTGCTCGATGAGCTGGCCAAGGCCAATGCCCTCGATGGG
CAGTCTTCGGATTTCCAGTTCGATGAGCTCGATGGCACCGTAGAGGAGGTGTCGGATGATGACGCTGGCGATGCCTCCAG
CGATGCCGATGATGCGCCGGTGGTGCGGTTTTTGCAGAAAATGATCATTGATGCCTACCGTCTGGGGGCTTCAGACTTGC
ATTTCGAGCCGTATGAATTCAATTACCGTGTGCGTTTTCGCATTGACGGTGAGTTGCGTGAATATGCTGCACCGCCTGTT
TCCATCAAGGAGCGCCTGGCATCTCGAATTAAAGTGATCTCCCGATTGGATATCGCAGAGAAACGTATCCCGCAGGATGG
TCGCACCAAAATGCGCATTGGCGGAAAGTCCATCGATTTTCGTGTCAGTACCTTGCCTACACTGTTTGGCGAGAAAATCG
TCATTCGTATTCTGGATTCCAGCAACGCCAAAATCGGCATTGATGCGCTGGGCTACGAGCCGATCGAAAAGGAGCGCCTG
CTCACGGCCATTGAGCGCCCCTATGGCATGGTGCTGGTGACTGGGCCAACGGGATCGGGTAAAACGGTGTCCTTGTACAC
CTGTCTGAACCTGTTGAACAAACCAGGGGTGAATATTTCCACGGCTGAGGACCCCTCGGAAATCAACCTGCCGGGCGTGA
ATCAGGTCAACGTCAATGAAAAGGCTGGCTTGACGTTTGCTGCAGCCCTGAAATCGTTTTTGCGCCAGGATCCCGACATC
ATCATGGTGGGTGAGATCCGTGACCTGGAAACGGCGGACATCGCCATCAAGGCCGCCCAAACTGGGCATTTGGTTTTGTC
CACGCTGCACACCAACGATGCCCCGACCACATTGACTCGCTTGCGCAACATGGGGATTGCTCCTTTCAATATTGCTTCCA
GCGTGATTCTGATCACTGCGCAGCGCCTGGCGCGTCGTCTTTGTAGTCAGTGCAGGGAAGAGACCGATATCCCAGTGGAT
GCTTTGATCGATGCGGGCTTTAATTACGATGATCTGGATGGCTCCTGGGTCCCTTATAAACCAGTGGGTTGCTCGGCGTG
CAACAACGGCTACAAAGGTCGAGTGGGTATTTATCAGGTGATGCCCATCAGTGAGGAAATCCAGCGCATTATCCTCAATG
ATGGTTCCGCAATCGAAATCCAGGAGCAGGCTGAAAAAGAAGGCGTGCGCACACTTCGCCAGTCAGGGCTGCAAAAAGTC
AAGCTGGGCGTGACTTCTTTGGAAGAAGTGCTGGCTGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.929

97.222

0.524
  pilF Neisseria gonorrhoeae MS11

54.122

96.875

0.524
  pilB Acinetobacter baylyi ADP1

53.539

95.66

0.512
  pilB Legionella pneumophila strain ERS1305867

50.96

99.479

0.507
  pilB Vibrio cholerae strain A1552

47.81

95.139

0.455
  pilB Vibrio campbellii strain DS40M4

45.971

94.792

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

45.604

94.792

0.432
  pilF Thermus thermophilus HB27

39.388

96.528

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.768

95.833

0.372

Multiple sequence alignment