Detailed information NTG1001394

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABFV68_RS12120
• Genome accession: NZ_CP156029
• Coordinates: 2509704..2510318 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CH1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2504704..2515318

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFV68_RS12090 (ABFV68_12090)	rpsT	2505007..2505273 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABFV68_RS12095 (ABFV68_12095)	holA	2505290..2506331 (-)	1042	Protein_2334	DNA polymerase III subunit delta	-
ABFV68_RS12100 (ABFV68_12100)	-	2506389..2506522 (-)	134	Protein_2335	hypothetical protein	-
ABFV68_RS12105 (ABFV68_12105)	-	2506563..2506697 (+)	135	WP_003152870.1	YqzM family protein	-
ABFV68_RS12110 (ABFV68_12110)	comEC	2506716..2509067 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABFV68_RS12115 (ABFV68_12115)	-	2509068..2509637 (-)	570	WP_003152868.1	ComE operon protein 2	-
ABFV68_RS12120 (ABFV68_12120)	comEA	2509704..2510318 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABFV68_RS12125 (ABFV68_12125)	comER	2510377..2511198 (+)	822	WP_053103971.1	late competence protein ComER	-
ABFV68_RS12130 (ABFV68_12130)	-	2511267..2512004 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
ABFV68_RS12135 (ABFV68_12135)	rsfS	2512001..2512357 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABFV68_RS12140 (ABFV68_12140)	yqeK	2512375..2512935 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABFV68_RS12145 (ABFV68_12145)	-	2512925..2513494 (-)	570	WP_042635364.1	nicotinate-nucleotide adenylyltransferase	-
ABFV68_RS12150 (ABFV68_12150)	yhbY	2513505..2513795 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ABFV68_RS12155 (ABFV68_12155)	aroE	2513789..2514631 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=1001394 ABFV68_RS12120 WP_032866693.1 2509704..2510318(-) (comEA) [Bacillus velezensis strain CH1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1001394 ABFV68_RS12120 WP_032866693.1 2509704..2510318(-) (comEA) [Bacillus velezensis strain CH1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363