Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU78_RS05350 Genome accession   NZ_CP156011
Coordinates   1265215..1266948 (+) Length   577 a.a.
NCBI ID   WP_227971327.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain CFBP 6943     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1260215..1271948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU78_RS05325 (ABFU78_05325) coaE 1261125..1261748 (-) 624 WP_274316878.1 dephospho-CoA kinase -
  ABFU78_RS05330 (ABFU78_05330) - 1261762..1262625 (-) 864 WP_040940797.1 A24 family peptidase -
  ABFU78_RS05335 (ABFU78_05335) pilC 1262632..1263891 (-) 1260 WP_407363956.1 type II secretion system F family protein Machinery gene
  ABFU78_RS05340 (ABFU78_05340) - 1264260..1264676 (+) 417 WP_227971328.1 pilin -
  ABFU78_RS05345 (ABFU78_05345) - 1264765..1265175 (+) 411 WP_274316877.1 pilin -
  ABFU78_RS05350 (ABFU78_05350) pilB 1265215..1266948 (+) 1734 WP_227971327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU78_RS05355 (ABFU78_05355) pilR 1267366..1268760 (-) 1395 WP_042594919.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU78_RS05360 (ABFU78_05360) - 1268970..1270580 (-) 1611 WP_040940804.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62805.97 Da        Isoelectric Point: 5.7640

>NTDB_id=1001166 ABFU78_RS05350 WP_227971327.1 1265215..1266948(+) (pilB) [Xanthomonas campestris pv. campestris strain CFBP 6943]
MNVANSPNLVGITGIARRLVQDGALEEAAARTAMDQATAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTQEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1001166 ABFU78_RS05350 WP_227971327.1 1265215..1266948(+) (pilB) [Xanthomonas campestris pv. campestris strain CFBP 6943]
ATGAACGTGGCTAATTCTCCCAATCTAGTTGGAATTACTGGAATTGCTCGTCGTTTAGTCCAAGACGGAGCGTTGGAAGA
GGCTGCCGCTCGCACCGCAATGGACCAGGCTACGGCAGCCAAAATTCCCTTGCCGCAATGGTTCTCTGAGAAGAAGTTGG
TCACCGCATCCCAGCTCGCTGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGATGGATGTGTCGGTGTTCGATGCCAGT
CAGAATGCAGTCAAGCTGGTGAGTGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGA
TCCTCGTCGACGAGGATCAGATCCGGCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGG
GACGACGACGAGGGGATGGGAGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCACTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGTTGTTGAAGAACGTAGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAACTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGTTACGAGGCAGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCACTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCTTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGTAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCAGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

55.106

98.44

0.542
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.126

99.133

0.487
  pilF Neisseria gonorrhoeae MS11

48.592

98.44

0.478
  pilB Vibrio campbellii strain DS40M4

46.221

98.614

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.869

96.88

0.454

Multiple sequence alignment