Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU04_RS05255 Genome accession   NZ_CP155965
Coordinates   1259024..1260757 (+) Length   577 a.a.
NCBI ID   WP_228957556.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1254024..1265757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU04_RS05235 (ABFU04_05230) coaE 1255346..1255969 (-) 624 WP_228876559.1 dephospho-CoA kinase -
  ABFU04_RS05240 (ABFU04_05235) - 1255983..1256846 (-) 864 WP_228876553.1 A24 family peptidase -
  ABFU04_RS05245 (ABFU04_05240) pilC 1256853..1258106 (-) 1254 WP_225042505.1 type II secretion system F family protein Machinery gene
  ABFU04_RS05250 (ABFU04_05245) pilA2 1258418..1258867 (+) 450 WP_225042506.1 pilin Machinery gene
  ABFU04_RS05255 (ABFU04_05250) pilB 1259024..1260757 (+) 1734 WP_228957556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU04_RS05260 (ABFU04_05255) pilR 1261174..1262568 (-) 1395 WP_228957555.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU04_RS05265 (ABFU04_05260) - 1262777..1264387 (-) 1611 WP_228957554.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62865.09 Da        Isoelectric Point: 5.6058

>NTDB_id=1000389 ABFU04_RS05255 WP_228957556.1 1259024..1260757(+) (pilB) [Xanthomonas campestris pv. raphani strain BR11]
MNSLLTANLAGITGIARRLVQDGALEESVARTAMDQAGQAKVPLPQWFAEKKIVSAAQLASANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000389 ABFU04_RS05255 WP_228957556.1 1259024..1260757(+) (pilB) [Xanthomonas campestris pv. raphani strain BR11]
ATGAATTCGTTGTTGACCGCTAATCTGGCTGGCATAACGGGAATCGCTCGCCGCTTAGTTCAGGACGGCGCATTGGAGGA
GTCCGTTGCACGCACCGCTATGGACCAAGCTGGTCAAGCAAAAGTCCCGCTGCCGCAATGGTTTGCGGAGAAGAAGATTG
TTTCAGCAGCTCAATTAGCCTCGGCCAATGCAGTCGAGTTCGGCATGCCGCTGATGGATGTATCGGTGTTCGACGCCAGC
CAGAATGCCATGAAGCTGGTCAGTGAGGAGTTGCTCCAGAAATACCAGGTGCTGCCTCTGTTCAAACGCGGCAACCGCTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTTCATACCAACCTTGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACGTTGGAGCAGTGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTTGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCCACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAAATTGCGATAAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAAGGCCGCACCGGTATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.613

100

0.558
  pilB Acinetobacter baylyi ADP1

55.634

98.44

0.548
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilB Vibrio cholerae strain A1552

48.87

99.653

0.487
  pilF Neisseria gonorrhoeae MS11

48.415

98.44

0.477
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment