Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3659287..3659828 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag B6N50_RS18760 Protein ID WP_000615979.1
Coordinates 3659550..3659828 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag B6N50_RS18755 Protein ID WP_000729704.1
Coordinates 3659287..3659547 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS18735 (B6N50_18735) 3655357..3656142 - 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -
B6N50_RS18740 (B6N50_18740) 3656114..3657836 + 1723 Protein_3419 flagellar biosynthesis protein FlhA -
B6N50_RS18745 (B6N50_18745) 3657915..3658271 + 357 WP_047402977.1 type II toxin-antitoxin system HicB family antitoxin -
B6N50_RS18750 (B6N50_18750) 3658328..3659101 - 774 WP_122990173.1 C40 family peptidase -
B6N50_RS18755 (B6N50_18755) 3659287..3659547 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B6N50_RS18760 (B6N50_18760) 3659550..3659828 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B6N50_RS18765 (B6N50_18765) 3659984..3660724 + 741 WP_001225679.1 murein L,D-transpeptidase -
B6N50_RS18770 (B6N50_18770) 3660695..3661462 - 768 WP_000333380.1 class II glutamine amidotransferase -
B6N50_RS18775 (B6N50_18775) 3661671..3662249 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / tssA / hcp1/tssD1 3657894..3682366 24472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T75077 WP_000615979.1 NZ_CP020543:3659550-3659828 [Escherichia coli C]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T75077 NZ_CP020543:3659550-3659828 [Escherichia coli C]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT75077 WP_000729704.1 NZ_CP020543:3659287-3659547 [Escherichia coli C]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT75077 NZ_CP020543:3659287-3659547 [Escherichia coli C]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References