Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3658362..3658903 Replicon chromosome
Accession NZ_LT906474
Organism Escherichia coli strain NCTC122

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CKV98_RS18660 Protein ID WP_000615979.1
Coordinates 3658625..3658903 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CKV98_RS18655 Protein ID WP_000729704.1
Coordinates 3658362..3658622 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKV98_RS18635 3654432..3655217 - 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -
CKV98_RS18640 3655189..3656911 + 1723 Protein_3418 flagellar biosynthesis protein FlhA -
CKV98_RS18645 3656990..3657346 + 357 WP_047402977.1 type II toxin-antitoxin system HicB family antitoxin -
CKV98_RS18650 3657403..3658176 - 774 WP_122990173.1 C40 family peptidase -
CKV98_RS18655 3658362..3658622 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CKV98_RS18660 3658625..3658903 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CKV98_RS18665 3659059..3659799 + 741 WP_001225679.1 murein L,D-transpeptidase -
CKV98_RS18670 3659770..3660537 - 768 WP_000333380.1 class II glutamine amidotransferase -
CKV98_RS18675 3660746..3661324 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / tssA / hcp1/tssD1 3656969..3681441 24472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T293759 WP_000615979.1 NZ_LT906474:3658625-3658903 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT293759 WP_000729704.1 NZ_LT906474:3658362-3658622 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References