86709

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3638952..3639493 Replicon chromosome
Accession NZ_CP024090
Organism Escherichia coli strain WG5

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CSR56_RS18590 Protein ID WP_000615979.1
Coordinates 3639215..3639493 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CSR56_RS18585 Protein ID WP_000729704.1
Coordinates 3638952..3639212 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSR56_RS18565 3635022..3635807 - 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -
CSR56_RS18570 3635779..3637501 + 1723 Protein_3399 flagellar biosynthesis protein FlhA -
CSR56_RS18575 3637580..3637936 + 357 WP_047402977.1 type II toxin-antitoxin system HicB family antitoxin -
CSR56_RS18580 3637993..3638766 - 774 WP_122990173.1 C40 family peptidase -
CSR56_RS18585 3638952..3639212 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CSR56_RS18590 3639215..3639493 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CSR56_RS18595 3639649..3640389 + 741 WP_001225679.1 murein L,D-transpeptidase -
CSR56_RS18600 3640360..3641127 - 768 WP_000333380.1 class II glutamine amidotransferase -
CSR56_RS18605 3641336..3641914 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / tssA / hcp1/tssD1 3637559..3662031 24472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T86709 WP_000615979.1 NZ_CP024090:3639215-3639493 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T86709 NZ_CP024090:3639215-3639493 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT86709 WP_000729704.1 NZ_CP024090:3638952-3639212 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT86709 NZ_CP024090:3638952-3639212 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References