Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Xre-RelE |
| Location | 1777554..1778140 | Replicon | chromosome |
| Accession | AE015451 | ||
| Organism | Pseudomonas putida KT2440 | ||
Toxin (Protein)
| Gene name | graT | Uniprot ID | - |
| Locus tag | PP_1586 | Protein ID | AAN67207.1 |
| Coordinates | 1777866..1778144 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | graA | Uniprot ID | - |
| Locus tag | PP_1585 | Protein ID | AAN67206.1 |
| Coordinates | 1777558..1777857 (-) | Length | 100 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PP_1581 | 1772818..1774809 | + | 1992 | AAN67202.1 | BNR domain protein | - |
| PP_1582 | 1774821..1775897 | + | 1077 | AAN67203.1 | conserved protein of unknown function | - |
| PP_1583 | 1775925..1776401 | + | 477 | AAN67204.1 | putative Structural protein P5 | - |
| PP_1584 | 1776398..1776898 | + | 501 | AAN67205.1 | conserved protein of unknown function | - |
| PP_5476 | 1776964..1777266 | + | 303 | AMM02811.1 | conserved protein of unknown function | - |
| PP_1585 | 1777558..1777857 | - | 300 | AAN67206.1 | putative Antidote protein | Antitoxin |
| PP_1586 | 1777866..1778144 | - | 279 | AAN67207.1 | putative Killer protein | Toxin |
| PP_1587 | 1778341..1780041 | + | 1701 | AAN67208.1 | NhaP-type Na+/H+ and K+/H+ antiporters | - |
| PP_1588 | 1780147..1781343 | - | 1197 | AAN67209.1 | N-succinyl-L,L-diaminopimelate aminotransferase alternative | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10446.72 Da Isoelectric Point: 7.2450
>T6083 AAN67207.1 AE015451:c1778144-1777866 [Pseudomonas putida KT2440]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH
Download Length: 279 bp
>T6083 AE015451:c1778144-1777866 [Pseudomonas putida KT2440]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA
Antitoxin
Download Length: 100 a.a. Molecular weight: 10905.55 Da Isoelectric Point: 5.6793
>AT6083 AAN67206.1 AE015451:c1777857-1777558 [Pseudomonas putida KT2440]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA
Download Length: 300 bp
>AT6083 AE015451:c1777857-1777558 [Pseudomonas putida KT2440]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10032 | Pseudomonas aeruginosa PAO1 |
69.565 |
100 |
0.696 |
| T10161 | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE |
69.565 |
100 |
0.696 |
| T171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
41.111 |
97.826 |
0.402 |
| T6124 | Haemophilus influenzae 86-028NP |
36.957 |
100 |
0.37 |
| T6146 | Proteus vulgaris |
30.851 |
100 |
0.315 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10161 | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE |
55.556 |
100 |
0.556 |
| AT10032 | Pseudomonas aeruginosa PAO1 |
55.556 |
100 |
0.556 |
| AT171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
43.333 |
90.909 |
0.394 |
| AT6124 | Haemophilus influenzae 86-028NP |
42.308 |
78.788 |
0.333 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Hedvig Tamman et al. (2014) A moderate toxin, GraT, modulates growth rate and stress tolerance of Pseudomonas putida. Journal of Bacteriology 196(1):157-69. [PubMed:24163334]
(2) Sirli Rosendahl et al. (2021) The Disordered C-Terminus of the Chaperone DnaK Increases the Competitive Fitness of Pseudomonas putida and Facilitates the Toxicity of GraT. Microorganisms 9(2):375. [PubMed:33668424]
(3) Ariel Talavera et al. (2019) A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nature Communications 10(1):972. [PubMed:30814507]
(4) Andres Ainelo et al. (2016) The toxin GraT inhibits ribosome biogenesis. Molecular Microbiology 100(4):719-34. [PubMed:26833678]
(5) Andres Ainelo et al. (2019) Pseudomonas putida Responds to the Toxin GraT by Inducing Ribosome Biogenesis Factors and Repressing TCA Cycle Enzymes. Toxins 11(2):103. [PubMed:30744127]