Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/- |
| Location | 5517562..5518157 | Replicon | chromosome |
| Accession | NZ_CP127126 | ||
| Organism | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | V6ALY3 |
| Locus tag | P4A98_RS25575 | Protein ID | WP_003113526.1 |
| Coordinates | 5517879..5518157 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | P4A98_RS25570 | Protein ID | WP_003133769.1 |
| Coordinates | 5517562..5517867 (-) | Length | 102 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| P4A98_RS25535 (P4A98_25535) | 5512703..5513551 | + | 849 | WP_003095007.1 | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | - |
| P4A98_RS25545 (P4A98_25545) | 5513718..5514659 | + | 942 | WP_003099281.1 | ribose-phosphate pyrophosphokinase | - |
| P4A98_RS25550 (P4A98_25550) | 5514776..5515390 | + | 615 | WP_003095013.1 | 50S ribosomal protein L25/general stress protein Ctc | - |
| P4A98_RS25555 (P4A98_25555) | 5515432..5516016 | + | 585 | WP_003099278.1 | aminoacyl-tRNA hydrolase | - |
| P4A98_RS25560 (P4A98_25560) | 5516057..5517157 | + | 1101 | WP_003099270.1 | redox-regulated ATPase YchF | - |
| P4A98_RS25570 (P4A98_25570) | 5517562..5517867 | - | 306 | WP_003133769.1 | HigA family addiction module antitoxin | Antitoxin |
| P4A98_RS25575 (P4A98_25575) | 5517879..5518157 | - | 279 | WP_003113526.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| P4A98_RS25580 (P4A98_25580) | 5518210..5518338 | - | 129 | Protein_5053 | integrase | - |
| P4A98_RS25585 (P4A98_25585) | 5518486..5520714 | + | 2229 | WP_003141628.1 | TonB-dependent receptor | - |
| P4A98_RS25590 (P4A98_25590) | 5520784..5521431 | - | 648 | WP_003095021.1 | carbonate dehydratase | - |
| P4A98_RS25595 (P4A98_25595) | 5521493..5522731 | - | 1239 | WP_003141631.1 | C69 family dipeptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10646.19 Da Isoelectric Point: 7.8937
>T10161 WP_003113526.1 NZ_CP127126:c5518157-5517879 [Pseudomonas aeruginosa PA14]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH
Download Length: 279 bp
>T10161 NZ_CP127126:c5518157-5517879 [Pseudomonas aeruginosa PA14]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA
Antitoxin
Download Length: 102 a.a. Molecular weight: 11214.73 Da Isoelectric Point: 4.6081
>AT10161 WP_003133769.1 NZ_CP127126:c5517867-5517562 [Pseudomonas aeruginosa PA14]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG
Download Length: 306 bp
>AT10161 NZ_CP127126:c5517867-5517562 [Pseudomonas aeruginosa PA14]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10032 | Pseudomonas aeruginosa PAO1 |
100 |
100 |
1 |
| T6083 | Pseudomonas putida KT2440 |
69.565 |
100 |
0.696 |
| T171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
40.964 |
90.217 |
0.37 |
| T6124 | Haemophilus influenzae 86-028NP |
37.179 |
84.783 |
0.315 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10032 | Pseudomonas aeruginosa PAO1 |
98.02 |
100 |
0.98 |
| AT6083 | Pseudomonas putida KT2440 |
55.556 |
100 |
0.556 |
Multiple sequence alignment
References
(1) Yueying Zhang et al. (2018) HigB Reciprocally Controls Biofilm Formation and the Expression of Type III Secretion System Genes through Influencing the Intracellular c-di-GMP Level in Pseudomonas aeruginosa. Toxins 10(11):424. [PubMed:30355991]