Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 5242254..5242849 Replicon chromosome
Accession NC_002516
Organism Pseudomonas aeruginosa PAO1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag - Protein ID -
Coordinates 5242571..5242849 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q9HVC1
Locus tag PA4674 Protein ID NP_253363.1
Coordinates 5242254..5242559 (-) Length 102 a.a.

Genomic Context

PA4669PA4670PA4671PA4672PA4673PA4675PA4674-PA4676PA4677
Location: 5237393..5238241 (849 bp)
Type: Others
Protein ID: NP_253358.1
Location: 5238408..5239349 (942 bp)
Type: Others
Protein ID: NP_253359.1
Location: 5239466..5240080 (615 bp)
Type: Others
Protein ID: NP_253360.1
Location: 5240122..5240706 (585 bp)
Type: Others
Protein ID: NP_253361.1
Location: 5240747..5241847 (1101 bp)
Type: Others
Protein ID: NP_253362.1
Location: 5243178..5245406 (2229 bp)
Type: Others
Protein ID: NP_253364.1
Location: 5242254..5242559 (306 bp)
Type: Antitoxin
Protein ID: NP_253363.1
Location: 5242571..5242849 (279 bp)
Type: Toxin
Protein ID: -
Location: 5245476..5246123 (648 bp)
Type: Others
Protein ID: NP_253365.1
Location: 5246185..5247423 (1239 bp)
Type: Others
Protein ID: NP_253366.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
PA46695237393..5238241+849NP_253358.14-diphosphocytidyl-2C-methyl-D-erythritol kinase-
PA46705238408..5239349+942NP_253359.1ribose-phosphate pyrophosphokinase-
PA46715239466..5240080+615NP_253360.150S ribosomal protein L25/general stress protein Ctc-
PA46725240122..5240706+585NP_253361.1peptidyl-tRNA hydrolase-
PA46735240747..5241847+1101NP_253362.1GTP-dependent nucleic acid-binding protein EngD-
PA46745242254..5242559-306NP_253363.1hypothetical proteinAntitoxin
-5242571..5242849-279--Toxin
PA46755243178..5245406+2229NP_253364.1TonB-dependent receptor-
PA46765245476..5246123-648NP_253365.1carbonic anhydrase-
PA46775246185..5247423-1239NP_253366.1hypothetical protein-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
No matching records found


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T10032 - NC_002516:c5242849-5242571 [Pseudomonas aeruginosa PAO1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T10032 NC_002516:c5242849-5242571 [Pseudomonas aeruginosa PAO1]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT10032 NP_253363.1 NC_002516:c5242559-5242254 [Pseudomonas aeruginosa PAO1]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT10032 NC_002516:c5242559-5242254 [Pseudomonas aeruginosa PAO1]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T10161Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE

100

100

1

T6083Pseudomonas putida KT2440

69.565

100

0.696

T171Vibrio cholerae O1 biovar El Tor str. N16961

40.964

90.217

0.37

T6124Haemophilus influenzae 86-028NP

37.179

84.783

0.315
Showing 1 to 4 of 4 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT10161Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE

98.02

100

0.98

AT6083Pseudomonas putida KT2440

55.556

100

0.556
Showing 1 to 2 of 2 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
No matching records found

Antitoxin

Loading, please wait
Source
ID
Structure
PDB6JPI
PDB6LB3
PDB7CSY
PDB7CSV
PDB7CSW
AlphaFold DBQ9HVC1

References

(1) Yingjie Song et al. (2023) Comparative analysis of five type II TA systems identified in Pseudomonas aeruginosa reveals their contributions to persistence and intracellular survival. Frontiers in Cellular And Infection Microbiology 13:1127786. [PubMed:36844395] experimental

(2) Inga Songailiene et al. (2020) HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Molecular Cell 80(6):955-970.e7. [PubMed:33290744] experimental

(3) Yingjie Song et al. (2021) Pseudomonas aeruginosa antitoxin HigA functions as a diverse regulatory factor by recognizing specific pseudopalindromic DNA motifs. Environmental Microbiology 23(3):1541-1558. [PubMed:33346387] experimental

(4) Ying Liu et al. (2019) Structural Insights Into the Transcriptional Regulation of HigBA Toxin-Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa. Frontiers in Microbiology 10:3158. [PubMed:32038588] 3D_structure

(5) Yunxue Guo et al. (2019) Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa. Environmental Microbiology 21(8):2707-2723. [PubMed:30882983] experimental


experimental experimental literature
3D_structure protein structure literature