Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/- |
| Location | 5242254..5242849 | Replicon | chromosome |
| Accession | NC_002516 | ||
| Organism | Pseudomonas aeruginosa PAO1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | 5242571..5242849 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | Q9HVC1 |
| Locus tag | PA4674 | Protein ID | NP_253363.1 |
| Coordinates | 5242254..5242559 (-) | Length | 102 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PA4669 | 5237393..5238241 | + | 849 | NP_253358.1 | 4-diphosphocytidyl-2C-methyl-D-erythritol kinase | - |
| PA4670 | 5238408..5239349 | + | 942 | NP_253359.1 | ribose-phosphate pyrophosphokinase | - |
| PA4671 | 5239466..5240080 | + | 615 | NP_253360.1 | 50S ribosomal protein L25/general stress protein Ctc | - |
| PA4672 | 5240122..5240706 | + | 585 | NP_253361.1 | peptidyl-tRNA hydrolase | - |
| PA4673 | 5240747..5241847 | + | 1101 | NP_253362.1 | GTP-dependent nucleic acid-binding protein EngD | - |
| PA4674 | 5242254..5242559 | - | 306 | NP_253363.1 | hypothetical protein | Antitoxin |
| - | 5242571..5242849 | - | 279 | - | - | Toxin |
| PA4675 | 5243178..5245406 | + | 2229 | NP_253364.1 | TonB-dependent receptor | - |
| PA4676 | 5245476..5246123 | - | 648 | NP_253365.1 | carbonic anhydrase | - |
| PA4677 | 5246185..5247423 | - | 1239 | NP_253366.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10646.19 Da Isoelectric Point: 7.8937
>T10032 - NC_002516:c5242849-5242571 [Pseudomonas aeruginosa PAO1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH
Download Length: 279 bp
>T10032 NC_002516:c5242849-5242571 [Pseudomonas aeruginosa PAO1]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA
Antitoxin
Download Length: 102 a.a. Molecular weight: 11247.81 Da Isoelectric Point: 5.0747
>AT10032 NP_253363.1 NC_002516:c5242559-5242254 [Pseudomonas aeruginosa PAO1]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG
Download Length: 306 bp
>AT10032 NC_002516:c5242559-5242254 [Pseudomonas aeruginosa PAO1]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10161 | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE |
100 |
100 |
1 |
| T6083 | Pseudomonas putida KT2440 |
69.565 |
100 |
0.696 |
| T171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
40.964 |
90.217 |
0.37 |
| T6124 | Haemophilus influenzae 86-028NP |
37.179 |
84.783 |
0.315 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10161 | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE |
98.02 |
100 |
0.98 |
| AT6083 | Pseudomonas putida KT2440 |
55.556 |
100 |
0.556 |
Multiple sequence alignment
References
(1) Yingjie Song et al. (2023) Comparative analysis of five type II TA systems identified in Pseudomonas aeruginosa reveals their contributions to persistence and intracellular survival. Frontiers in Cellular And Infection Microbiology 13:1127786. [PubMed:36844395]
(2) Inga Songailiene et al. (2020) HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. Molecular Cell 80(6):955-970.e7. [PubMed:33290744]
(3) Yingjie Song et al. (2021) Pseudomonas aeruginosa antitoxin HigA functions as a diverse regulatory factor by recognizing specific pseudopalindromic DNA motifs. Environmental Microbiology 23(3):1541-1558. [PubMed:33346387]
(4) Ying Liu et al. (2019) Structural Insights Into the Transcriptional Regulation of HigBA Toxin-Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa. Frontiers in Microbiology 10:3158. [PubMed:32038588]
(5) Yunxue Guo et al. (2019) Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa. Environmental Microbiology 21(8):2707-2723. [PubMed:30882983]