Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Xre-RelE |
| Location | 1756100..1756739 | Replicon | chromosome |
| Accession | NC_007146 | ||
| Organism | Haemophilus influenzae 86-028NP | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | Q4QJX7 |
| Locus tag | NTHI_RS08835 | Protein ID | WP_005650215.1 |
| Coordinates | 1756434..1756739 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | NTHI_RS08830 | Protein ID | WP_005650217.1 |
| Coordinates | 1756100..1756423 (-) | Length | 108 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NTHI_RS09945 (NTHI1907) | 1751719..1751892 | - | 174 | WP_011272700.1 | DUF5363 domain-containing protein | - |
| NTHI_RS08815 (NTHI1908) | 1751889..1753859 | - | 1971 | WP_011272701.1 | tRNA(Met) cytidine acetyltransferase TmcA | - |
| NTHI_RS08820 (NTHI1910) | 1753864..1754205 | - | 342 | WP_005652996.1 | SirB2 family protein | - |
| NTHI_RS08825 (NTHI1911) | 1754267..1755937 | - | 1671 | WP_005650218.1 | energy-dependent translational throttle protein EttA | - |
| NTHI_RS08830 (NTHI1912) | 1756100..1756423 | - | 324 | WP_005650217.1 | HigA family addiction module antidote protein | Antitoxin |
| NTHI_RS08835 (NTHI1913) | 1756434..1756739 | - | 306 | WP_005650215.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| NTHI_RS08840 (NTHI1914) | 1757064..1757684 | + | 621 | WP_005694310.1 | zinc transporter binding subunit ZevA | - |
| NTHI_RS08845 (NTHI1915) | 1757687..1758655 | + | 969 | WP_011272702.1 | zinc transporter permease subunit ZevB | - |
| NTHI_RS08855 (NTHI1917) | 1759269..1761308 | + | 2040 | WP_011272703.1 | excinuclease ABC subunit B | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 12301.93 Da Isoelectric Point: 9.0853
>T6124 WP_005650215.1 NC_007146:c1756739-1756434 [Haemophilus influenzae 86-028NP]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL
Download Length: 306 bp
>T6124 NC_007146:c1756739-1756434 [Haemophilus influenzae 86-028NP]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA
Antitoxin
Download Length: 108 a.a. Molecular weight: 12347.17 Da Isoelectric Point: 6.8804
>AT6124 WP_005650217.1 NC_007146:c1756423-1756100 [Haemophilus influenzae 86-028NP]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM
Download Length: 324 bp
>AT6124 NC_007146:c1756423-1756100 [Haemophilus influenzae 86-028NP]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
46.154 |
95.789 |
0.442 |
| T6083 | Pseudomonas putida KT2440 |
36.957 |
100 |
0.37 |
| T10032 | Pseudomonas aeruginosa PAO1 |
37.179 |
84.783 |
0.315 |
| T10161 | Pseudomonas aeruginosa PA14 strain MA3 isolate DTU_MIE |
37.179 |
84.783 |
0.315 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT171 | Vibrio cholerae O1 biovar El Tor str. N16961 |
47.826 |
87.619 |
0.419 |
| AT6083 | Pseudomonas putida KT2440 |
42.308 |
78.788 |
0.333 |
Multiple sequence alignment
References
(1) Dabin Ren et al. (2014) The ToxAvapA toxin-antitoxin locus contributes to the survival of nontypeable Haemophilus influenzae during infection. PloS One 9(3):e91523. [PubMed:24621787]