6146

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Xre-RelE
Location 194764..195356 Replicon plasmid Rts1
Accession NC_003905
Organism Proteus vulgaris

Toxin (Protein)


Gene name higB Uniprot ID Q7A225
Locus tag HXF74_RS01175 Protein ID WP_011039853.1
Coordinates 194764..195042 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID L7Z874
Locus tag HXF74_RS01180 Protein ID WP_011039854.1
Coordinates 195042..195356 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXF74_RS01150 (Rts1_253) 190571..191479 + 909 WP_011039846.1 hypothetical protein -
HXF74_RS01155 (Rts1_255) 191765..192094 + 330 WP_011039848.1 hypothetical protein -
HXF74_RS01160 (Rts1_256) 192091..192543 + 453 WP_109910770.1 hypothetical protein -
HXF74_RS01165 (Rts1_258) 193584..193826 + 243 WP_011039851.1 hypothetical protein -
HXF74_RS01170 (Rts1_259) 194270..194593 + 324 WP_109910769.1 hypothetical protein -
HXF74_RS01175 (Rts1_260) 194764..195042 + 279 WP_011039853.1 type II toxin-antitoxin system toxin endoribonuclease HigB Toxin
HXF74_RS01180 (Rts1_261) 195042..195356 + 315 WP_011039854.1 type II toxin antitoxin system addiction module antidote protein HigA Antitoxin
HXF74_RS01185 (Rts1_262) 195594..195983 + 390 WP_172691635.1 hypothetical protein -
HXF74_RS01190 196014..196184 - 171 WP_176455176.1 hypothetical protein -
HXF74_RS01195 (Rts1_264) 196183..197103 + 921 WP_011039857.1 hypothetical protein -
HXF74_RS01200 (Rts1_265) 197135..197311 + 177 WP_011039858.1 hypothetical protein -
HXF74_RS01205 (Rts1_266) 197337..197774 + 438 WP_109910766.1 plasmid protein -
HXF74_RS01210 (Rts1_267) 197792..198514 + 723 WP_109910765.1 hypothetical protein -
HXF74_RS01215 (Rts1_268) 198794..199309 + 516 WP_109910764.1 hypothetical protein -
HXF74_RS01220 199617..199931 + 315 WP_109910763.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia - 1..217182 217182


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-67)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10742.22 Da        Isoelectric Point: 8.4392

>T6146 WP_011039853.1 NC_003905:194764-195042 [Proteus vulgaris]
MIKSFKHKGLKLLFEKGVTSGVPAQDVDRINDRLQAIDTATEIGELNRQIYKLHPLKGDREGYWSITVRANWRITFQFIN
GDAYILNYEDYH

Download         Length: 279 bp

>T6146 NC_003905:194764-195042 [Proteus vulgaris]
ATGATAAAATCTTTCAAGCACAAAGGATTGAAACTGCTTTTTGAAAAGGGTGTTACTTCTGGTGTGCCTGCGCAAGATGT
AGACAGAATCAATGACCGTTTGCAGGCCATCGATACAGCGACAGAGATTGGTGAACTAAACCGCCAGATTTACAAATTAC
ATCCATTAAAGGGGGATCGGGAAGGTTACTGGTCTATCACTGTCCGGGCGAATTGGCGCATTACTTTTCAGTTCATTAAC
GGTGATGCTTACATTTTAAATTATGAGGATTATCACTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11536.99 Da        Isoelectric Point: 6.7241

>AT6146 WP_011039854.1 NC_003905:195042-195356 [Proteus vulgaris]
MRQFKVSHPGEMIARDLEDMGVSGRRFAHNIGVTPATVSRLLAGKTALTPSLSIRIAAALGSTPEFWLRLQSNYDLRQLE
NQIDTSGIVLYGESNEQQQNAQEH

Download         Length: 315 bp

>AT6146 NC_003905:195042-195356 [Proteus vulgaris]
ATGAGACAATTCAAGGTTTCACATCCGGGTGAGATGATAGCCCGCGATTTAGAGGATATGGGAGTGAGTGGTCGTCGTTT
TGCTCACAATATTGGTGTTACACCAGCAACCGTATCCCGTCTACTTGCGGGGAAAACTGCGTTGACCCCTTCTCTTTCGA
TTCGTATTGCTGCGGCACTGGGGAGTACGCCTGAGTTTTGGTTACGGCTACAGAGCAATTATGATCTGCGCCAGTTAGAA
AACCAAATCGATACATCCGGGATCGTCTTGTACGGTGAGTCGAACGAACAGCAGCAGAACGCGCAAGAGCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6083 Pseudomonas putida KT2440

30.851

100

0.315

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT171 Vibrio cholerae O1 biovar El Tor str. N16961

44.595

71.154

0.317

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 4MCT
PDB 4YPB
PDB 4MCX
PDB 4YY3
PDB 4PX8
PDB 4ZSN
PDB 4W4G
PDB 5IWH
PDB 5IXL
PDB 4YZV

Antitoxin

Source ID Structure
AlphaFold DB A0A379FY56

References

(1) Jennifer M Hurley et al. (2009) Bacterial toxin HigB associates with ribosomes and mediates translation-dependent mRNA cleavage at A-rich sites. The Journal of Biological Chemistry 284(28):18605-13. [PubMed:19423702] experimental

(2) Marc A Schureck et al. (2019) Structural basis of transcriptional regulation by the HigA antitoxin. Molecular Microbiology 111(6):1449-1462. [PubMed:30793388] experimental


experimental experimental literature