Detailed information of component protein


Summary

Component ID T6CP015739
Component protein type TssI
T6SS ID (Type) T6SS01261 experimental (Type i5)
Strain Rhizobium etli bv. mimosae str. Mim1
Replicon plasmid pRetMIM1f
Sequence Protein sequence (755 a.a.); Nucleotide sequence (2265 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) REMIM1_RS31825
Coordinate (Strand) 513253..515517 (+)
NCBI ID WP_020919514.1
RefSeq NC_021911
Uniprot ID S5S8B9_RHIET
KEGG ID rel:REMIM1_PF00480
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.1e-76 37..347
Phage_base_V PF04717.14 2.5e-12 400..467
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-754 MDDLQSLAADFIQANRILKIVSPLGEDQLLPERVTIEEGISSLFEIKISARAKKPSVKPEELIGRLMDVSVEVQQGDGEGALRRPFNGLVTELNEGPPITRGLRSYSMVLRPQMWLLSRRSDCRIWMDKTSVDIVETLFSEHGIPAPDTSGVITPPPPQHYSVQWNETDLAFLTRRFEEDGLFYWFSHEDGKHKLHVADSAQSWLGPSPSAQGEGRVRLAQGSADRNHITEWSRRYSYVPGQRAGADWNFETPRMVPGTMTPSLVQLPEASKRELYEYPARISSVAEAERAEKLRMQASEADHDRVFGGSTSRILEAGRRFTPYEVAHPDHVYEEHVVVKATHTVVDRSYETNSNEPEYTNTFEAVPSRVPMTPHRETKRPRIEGTQVAIVAGPSGEEIHPDQYGRIKLWFPWDRKAKKDGSDTCWVRVSQVWGGGTWGAQVIPRIGMEVMVAFVDGDPDRPLVTGVVPNPSNTVPYDLPANKTRMVMRSNTHKGTGFNEMTFEDEAGQENMFFHAQKDQTTRVLNDRIKRVDRHEVSAIGGNRAVEVSGNQKHEIGGSVNTVVGGTGPMAMALMGAVSGLSGHTAGLLAQAGQIAGGGGAGLAAFAGTVASSALGFLGAGGLGSREGVVSGPSPRADAGTALAGSGTGVGDAASGLFPMPGIMNTIVGAFKSDTIGIARAEQIGMSKVTNVGQTSLESVGKFKKIAVGEEFVIECGDSKFIMKANGEVIILGKTFNFVATEHFQMRGKPIDLN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9925 - -


  Protein sequence: 755 a.a.    

>T6CP015739 NC_021911:513253-515517 [Rhizobium etli bv. mimosae str. Mim1] [TssI]
MDDLQSLAADFIQANRILKIVSPLGEDQLLPERVTIEEGISSLFEIKISARAKKPSVKPEELIGRLMDVSVEVQQGDGEG
ALRRPFNGLVTELNEGPPITRGLRSYSMVLRPQMWLLSRRSDCRIWMDKTSVDIVETLFSEHGIPAPDTSGVITPPPPQH
YSVQWNETDLAFLTRRFEEDGLFYWFSHEDGKHKLHVADSAQSWLGPSPSAQGEGRVRLAQGSADRNHITEWSRRYSYVP
GQRAGADWNFETPRMVPGTMTPSLVQLPEASKRELYEYPARISSVAEAERAEKLRMQASEADHDRVFGGSTSRILEAGRR
FTPYEVAHPDHVYEEHVVVKATHTVVDRSYETNSNEPEYTNTFEAVPSRVPMTPHRETKRPRIEGTQVAIVAGPSGEEIH
PDQYGRIKLWFPWDRKAKKDGSDTCWVRVSQVWGGGTWGAQVIPRIGMEVMVAFVDGDPDRPLVTGVVPNPSNTVPYDLP
ANKTRMVMRSNTHKGTGFNEMTFEDEAGQENMFFHAQKDQTTRVLNDRIKRVDRHEVSAIGGNRAVEVSGNQKHEIGGSV
NTVVGGTGPMAMALMGAVSGLSGHTAGLLAQAGQIAGGGGAGLAAFAGTVASSALGFLGAGGLGSREGVVSGPSPRADAG
TALAGSGTGVGDAASGLFPMPGIMNTIVGAFKSDTIGIARAEQIGMSKVTNVGQTSLESVGKFKKIAVGEEFVIECGDSK
FIMKANGEVIILGKTFNFVATEHFQMRGKPIDLN*

  Nucleotide sequence: 2265 bp    

>T6CP015739 NC_021911:513253-515517 [Rhizobium etli bv. mimosae str. Mim1] [TssI]
ATGGACGATTTGCAATCCCTTGCCGCCGACTTCATCCAGGCGAACCGTATTCTGAAGATCGTCTCGCCGCTTGGCGAGGA
CCAGCTTCTGCCGGAGCGGGTGACGATCGAGGAAGGCATTTCTTCGCTCTTCGAAATCAAGATTTCGGCGCGTGCCAAGA
AGCCGTCGGTTAAGCCGGAGGAACTGATCGGCCGGCTAATGGATGTCTCCGTGGAGGTGCAGCAGGGCGATGGCGAAGGC
GCCCTGCGCCGGCCGTTCAACGGCTTGGTGACCGAACTCAACGAGGGGCCGCCGATCACCCGCGGGCTGCGCTCCTACTC
CATGGTGCTGCGGCCGCAGATGTGGCTCTTATCGCGCCGCTCCGACTGCCGTATCTGGATGGACAAGACCTCGGTCGACA
TCGTCGAAACGCTGTTTTCCGAACATGGAATCCCGGCACCCGATACGTCGGGCGTCATCACGCCACCGCCGCCGCAGCAT
TATTCGGTGCAGTGGAACGAAACCGATCTTGCCTTCCTCACCCGCCGTTTCGAAGAGGACGGCCTGTTCTACTGGTTTTC
GCATGAGGACGGCAAACACAAGCTGCATGTGGCCGACAGCGCCCAATCCTGGCTCGGCCCCTCGCCATCGGCGCAGGGGG
AGGGTCGGGTTCGCCTGGCGCAGGGCTCGGCCGACCGCAATCACATCACCGAGTGGAGCCGGCGTTATTCCTATGTGCCG
GGCCAGCGCGCCGGCGCCGACTGGAACTTCGAAACCCCACGCATGGTGCCCGGCACGATGACCCCGTCTCTGGTGCAGCT
GCCGGAAGCGAGCAAGCGCGAGCTTTACGAATATCCGGCGCGCATCTCTTCCGTCGCCGAGGCGGAGCGGGCGGAGAAAC
TGAGGATGCAGGCCTCCGAGGCCGATCACGACCGGGTCTTCGGCGGCTCGACATCCCGCATCCTGGAGGCCGGACGCCGC
TTCACACCTTATGAGGTCGCTCATCCCGACCATGTCTATGAGGAGCATGTGGTGGTCAAGGCGACCCATACGGTCGTCGA
CCGCTCCTATGAGACCAACAGCAACGAGCCGGAATATACCAATACCTTCGAGGCGGTGCCGTCGCGGGTGCCGATGACGC
CGCACCGCGAGACGAAGCGGCCGCGCATCGAGGGCACGCAGGTGGCGATCGTCGCCGGCCCTTCCGGCGAGGAGATCCAT
CCCGACCAATACGGCCGCATCAAGCTGTGGTTCCCCTGGGACCGCAAGGCCAAGAAGGACGGCTCCGACACTTGCTGGGT
GCGGGTCAGCCAGGTCTGGGGCGGCGGCACCTGGGGCGCGCAGGTTATCCCGCGCATCGGCATGGAGGTGATGGTCGCCT
TCGTCGACGGCGACCCCGACCGGCCGCTGGTAACAGGCGTGGTGCCCAATCCCAGCAACACGGTTCCCTACGATCTGCCT
GCCAACAAGACCCGCATGGTCATGCGCTCCAACACCCACAAGGGCACCGGCTTCAACGAAATGACCTTCGAGGACGAGGC
CGGCCAGGAGAACATGTTCTTCCATGCGCAGAAGGACCAGACGACGCGCGTGCTGAATGACCGCATCAAACGCGTCGACC
GGCATGAGGTCTCGGCCATCGGTGGCAACCGCGCGGTGGAGGTGAGTGGCAATCAGAAGCACGAGATCGGCGGTTCGGTG
AACACGGTCGTCGGCGGCACCGGGCCGATGGCGATGGCGCTGATGGGGGCGGTCAGTGGTCTCTCCGGACATACCGCCGG
GCTCCTGGCCCAGGCCGGGCAAATTGCAGGCGGCGGCGGGGCAGGGCTTGCGGCCTTCGCCGGCACGGTTGCGTCTTCGG
CGCTCGGCTTTCTCGGCGCTGGCGGGCTGGGCAGCCGTGAGGGCGTGGTGTCGGGACCGTCGCCAAGAGCGGATGCGGGC
ACGGCGCTTGCCGGTTCCGGCACGGGTGTTGGCGATGCGGCTTCGGGCCTTTTCCCAATGCCCGGCATCATGAACACAAT
CGTCGGCGCCTTCAAATCCGACACGATCGGCATTGCCCGCGCCGAGCAGATCGGCATGAGCAAGGTCACCAATGTCGGTC
AGACCTCGCTGGAGAGCGTCGGCAAGTTCAAGAAGATCGCGGTCGGCGAGGAATTTGTCATCGAATGCGGCGATTCCAAA
TTCATCATGAAAGCCAACGGCGAAGTGATCATCCTCGGCAAGACGTTCAACTTCGTTGCGACCGAACACTTCCAGATGCG
CGGCAAGCCGATCGACCTGAACTGA