SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015736
Component protein type	TssA
T6SS ID (Type)	T6SS01261 (Type i5)
Strain	Rhizobium etli bv. mimosae str. Mim1
Replicon	plasmid pRetMIM1f
Sequence	Protein sequence (381 a.a.); Nucleotide sequence (1143 bp)
Reference	[1] Salinero-Lanzarote A, Pacheco-Moreno A, Domingo-Serrano L, et al. The Type VI secretion system of Rhizobium etli Mim1 has a positive effect in symbiosis. FEMS Microbiol Ecol. 2019 May 1;95(5). pii: fiz054. doi: 10.1093/femsec/fiz054. PMID: 30977796

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	REMIM1_RS31800
Coordinate (Strand)	506486..507628 (-)
NCBI ID	WP_080655899.1
RefSeq	NC_021911
Uniprot ID	UPI0009B7C1DE
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	6.2e-26	34..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-380	MVDKSIQAAQNARRPQYVLIFEVFSSVNVREIVDPLAQNYPCGENVRNNTSLREIYYRIKDARNAARTAERSISPGDVITLAPAWHDVSNLGLQILSSRSKDIEVLAWLAEAQLRLRGFSGLRDVYVATASLLDKHFDALHSIGDSDFEERFAPFAGLNGVGGEGTLIQAIRLTSLIPDGKFAQFSLWDFQLSQRPNEAERRKELQQAAAEAGVARMSAHLNVVTECIAAFDRMIEILDKHCGDQAPPSSNTRNALQEAASAIRMLAGIEAPSVISETPASQGLEPLPAADIGESETVRMRPVSAEMIRSREEAFELLAAVGRYFRRTEPHSPISMSIETLVRRGRMDFSELLAELLPEQHARNAVLTAAGIQPVTDKGG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9781	-	-

Protein sequence: 381 a.a.

						>T6CP015736 NC_021911:c507628-506486 [Rhizobium etli bv. mimosae str. Mim1] [TssA]
MVDKSIQAAQNARRPQYVLIFEVFSSVNVREIVDPLAQNYPCGENVRNNTSLREIYYRIKDARNAARTAERSISPGDVIT
LAPAWHDVSNLGLQILSSRSKDIEVLAWLAEAQLRLRGFSGLRDVYVATASLLDKHFDALHSIGDSDFEERFAPFAGLNG
VGGEGTLIQAIRLTSLIPDGKFAQFSLWDFQLSQRPNEAERRKELQQAAAEAGVARMSAHLNVVTECIAAFDRMIEILDK
HCGDQAPPSSNTRNALQEAASAIRMLAGIEAPSVISETPASQGLEPLPAADIGESETVRMRPVSAEMIRSREEAFELLAA
VGRYFRRTEPHSPISMSIETLVRRGRMDFSELLAELLPEQHARNAVLTAAGIQPVTDKGG*					

Nucleotide sequence: 1143 bp

						>T6CP015736 NC_021911:c507628-506486 [Rhizobium etli bv. mimosae str. Mim1] [TssA]
ATGGTTGACAAGTCAATACAAGCCGCACAAAATGCTCGACGGCCGCAGTATGTTTTGATTTTTGAGGTATTTAGCAGCGT
GAATGTCCGCGAAATCGTAGATCCACTTGCACAAAATTATCCGTGCGGAGAAAACGTCCGGAATAACACTTCGTTGCGCG
AAATATACTATAGAATTAAGGACGCCAGAAACGCCGCACGTACAGCCGAACGTAGTATTTCACCGGGTGACGTTATTACG
CTTGCACCCGCTTGGCACGATGTTAGCAATCTTGGATTGCAAATTCTTTCTTCCAGGAGCAAGGACATAGAAGTATTGGC
CTGGCTTGCCGAAGCGCAGCTGCGGCTGCGCGGCTTCTCAGGATTGCGCGACGTCTATGTTGCAACCGCGTCGCTTCTAG
ACAAGCATTTCGACGCTTTGCACTCGATCGGAGACAGCGATTTCGAGGAACGGTTCGCTCCATTTGCAGGTCTTAACGGC
GTGGGCGGCGAAGGAACGCTTATCCAGGCTATTCGTTTGACATCGTTGATCCCGGACGGAAAATTCGCACAGTTTTCGCT
CTGGGATTTCCAGCTTTCGCAGCGGCCGAACGAAGCCGAGCGGCGCAAGGAATTGCAGCAGGCGGCGGCAGAAGCCGGTG
TCGCACGGATGTCGGCCCATCTCAACGTGGTGACAGAGTGCATCGCCGCCTTCGACCGCATGATCGAAATACTTGACAAA
CACTGTGGGGATCAGGCGCCACCTAGTTCGAATACACGCAATGCTTTGCAGGAAGCGGCCTCTGCAATCCGGATGCTCGC
CGGCATCGAGGCTCCCTCGGTCATATCAGAAACCCCTGCTTCGCAAGGCTTAGAGCCGCTGCCGGCAGCCGATATCGGCG
AGTCCGAGACCGTCCGCATGCGGCCCGTCAGCGCCGAGATGATTCGGTCACGCGAAGAGGCTTTCGAACTGCTCGCTGCC
GTTGGGCGCTATTTCCGCCGCACCGAACCCCATTCACCGATTTCTATGTCGATCGAGACCCTGGTGCGCCGCGGCCGCAT
GGATTTTTCCGAGCTTCTTGCCGAGCTCCTGCCTGAGCAGCACGCGCGCAATGCGGTGCTGACGGCGGCCGGCATACAGC
CCGTAACCGACAAGGGAGGCTAG					