Detailed information of component protein


Summary

Component ID T6CP015728
Component protein type TssL
T6SS ID (Type) T6SS01261 experimental (Type i5)
Strain Rhizobium etli bv. mimosae str. Mim1
Replicon plasmid pRetMIM1f
Sequence Protein sequence (513 a.a.); Nucleotide sequence (1539 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) REMIM1_RS31750
Coordinate (Strand) 494624..496162 (-)
NCBI ID WP_020919500.1
RefSeq NC_021911
Uniprot ID S5S8A9_RHIET
KEGG ID rel:REMIM1_PF00465
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2e-52 79..281
OmpA PF00691.22 4.5e-11 374..471
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-257 MKNEPASTWQSLPTIVELTEDGVKKKQSARKIAEMLDDVLDFPEGEKGTGPAAAPAKGGASVETLVKDFRFGAEDVPTLVRSAAPLLNLAHGLRYTEEQPADIDELRTVAIAAVGRYERDLASARISPERARAAHYVVCATVDDVVLSKAWGVRAGWARSGLVSTFHMDVTGGERVFDLLDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSARGALELARIRDSLYKTLLGQYGVFERELSPHWKGVSARHQPLRT
Transmembrane helix 258-280 AAALWTLLSVLMLILALGYLFFT
Outside 281-512 LSLNRASDGTFERLANLPPRETPSVLVKVPEPPREPQPVVVAQTPAPPPPPPQVKPPSRLDNLLAFLQPEVEKKLVTLSDSNGRLLVRISNSGLFATGSAEVSEKFHDLLQRIGGALAAEKFRAVVVGYTDNVPIRTVQFPSNWHLSEARAKAVGEILASFTGPGAILTEGRADSDPIAGNDTQEGREMNRRTEILVLTDPSERLSDAGLKTPPIEAETPNAPAATAGGAPR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9861 - -


  Protein sequence: 513 a.a.    

>T6CP015728 NC_021911:c496162-494624 [Rhizobium etli bv. mimosae str. Mim1] [TssL]
MKNEPASTWQSLPTIVELTEDGVKKKQSARKIAEMLDDVLDFPEGEKGTGPAAAPAKGGASVETLVKDFRFGAEDVPTLV
RSAAPLLNLAHGLRYTEEQPADIDELRTVAIAAVGRYERDLASARISPERARAAHYVVCATVDDVVLSKAWGVRAGWARS
GLVSTFHMDVTGGERVFDLLDHFHQSPGANKDLLLLIYLCLSLAFEGRTRVSARGALELARIRDSLYKTLLGQYGVFERE
LSPHWKGVSARHQPLRTAAALWTLLSVLMLILALGYLFFTLSLNRASDGTFERLANLPPRETPSVLVKVPEPPREPQPVV
VAQTPAPPPPPPQVKPPSRLDNLLAFLQPEVEKKLVTLSDSNGRLLVRISNSGLFATGSAEVSEKFHDLLQRIGGALAAE
KFRAVVVGYTDNVPIRTVQFPSNWHLSEARAKAVGEILASFTGPGAILTEGRADSDPIAGNDTQEGREMNRRTEILVLTD
PSERLSDAGLKTPPIEAETPNAPAATAGGAPR*

  Nucleotide sequence: 1539 bp    

>T6CP015728 NC_021911:c496162-494624 [Rhizobium etli bv. mimosae str. Mim1] [TssL]
ATGAAGAACGAACCCGCTTCCACCTGGCAAAGCCTGCCGACCATCGTCGAACTCACCGAGGATGGCGTGAAGAAAAAGCA
ATCCGCCCGGAAGATTGCGGAGATGCTGGACGACGTTCTCGATTTTCCCGAAGGCGAGAAGGGAACGGGACCAGCGGCAG
CGCCTGCGAAGGGCGGAGCCTCCGTCGAAACACTCGTCAAGGATTTCCGCTTCGGGGCCGAGGACGTGCCGACCCTGGTG
CGCTCGGCCGCGCCGCTGCTCAACCTTGCCCATGGCCTGCGCTACACCGAAGAGCAGCCCGCTGACATCGATGAACTCAG
GACCGTGGCGATCGCAGCCGTCGGCCGCTACGAGCGGGATCTCGCGAGCGCGCGCATTAGCCCCGAGCGGGCGCGTGCGG
CCCATTATGTCGTCTGCGCGACGGTCGACGACGTCGTGCTCAGCAAGGCTTGGGGCGTGCGCGCCGGATGGGCGCGTTCG
GGCCTCGTGTCCACCTTCCATATGGACGTGACCGGCGGCGAACGGGTGTTCGACCTGCTCGACCATTTCCACCAGAGCCC
CGGCGCCAACAAGGACCTGCTGCTCCTCATCTATCTCTGCCTGTCGCTCGCCTTCGAAGGCCGCACCCGCGTTTCCGCGC
GCGGCGCGCTGGAGCTCGCCCGCATCCGCGACAGTCTCTACAAGACGCTGCTCGGCCAGTACGGGGTCTTCGAACGGGAG
CTTTCCCCGCATTGGAAGGGCGTTTCCGCCCGGCACCAGCCGCTGCGCACCGCCGCCGCCCTTTGGACGCTGCTTTCCGT
CCTGATGCTGATCCTGGCTTTAGGCTACCTGTTCTTCACGCTCTCGCTGAACCGCGCCTCCGACGGCACGTTCGAGCGCC
TTGCCAACCTGCCGCCACGCGAGACGCCAAGCGTGCTGGTCAAGGTTCCAGAGCCGCCGCGCGAACCTCAGCCTGTGGTC
GTCGCGCAAACGCCGGCTCCTCCTCCGCCACCGCCGCAGGTCAAGCCGCCGAGCCGGCTCGATAATCTGCTCGCCTTCCT
CCAGCCGGAGGTCGAGAAGAAGCTCGTCACGCTGTCGGATTCGAACGGCAGGCTGCTCGTGCGCATCAGCAATTCCGGCC
TGTTTGCCACCGGCAGCGCCGAGGTCAGCGAGAAGTTCCACGATCTCCTGCAGCGGATCGGCGGCGCGCTAGCCGCGGAA
AAATTCCGCGCCGTCGTCGTTGGCTATACGGACAATGTGCCGATCCGAACCGTTCAATTCCCATCTAACTGGCACCTGTC
AGAAGCCCGCGCCAAGGCGGTCGGCGAAATCCTGGCATCCTTCACCGGGCCGGGGGCGATCCTGACCGAGGGACGGGCCG
ATAGCGATCCGATCGCCGGCAACGACACGCAGGAGGGCCGGGAGATGAACCGCCGCACCGAGATCCTGGTGCTGACCGAT
CCGAGCGAACGTCTGAGTGACGCCGGCTTGAAGACGCCGCCGATCGAGGCCGAAACACCGAATGCACCGGCTGCAACCGC
GGGAGGCGCTCCGCGATGA