Detailed information of component protein


Summary

Component ID T6CP015727
Component protein type TssM
T6SS ID (Type) T6SS01261 experimental (Type i5)
Strain Rhizobium etli bv. mimosae str. Mim1
Replicon plasmid pRetMIM1f
Sequence Protein sequence (1159 a.a.); Nucleotide sequence (3477 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) REMIM1_RS31745
Coordinate (Strand) 491148..494624 (-)
NCBI ID WP_020919499.1
RefSeq NC_021911
Uniprot ID S5SXC6_RHIET
KEGG ID rel:REMIM1_PF00464
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.2e-74 207..444
IcmF-related PF06761.14 5.1e-56 498..794
IcmF_C PF06744.14 1.4e-21 1037..1139
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MNPISYFYTLRSYVEAYAG
Transmembrane helix 20-42 LLGRRFISIIWVAAICVVIWFYG
Outside 43-56 YLAAYGDFKPLASA
Transmembrane helix 57-79 TARLTLIGVVIAAWLAYLLFTVI
Inside 80-434 RDGRRDKQLVEGIERDAEAEAAASQQAEVGEIHSRLKEALQLLRRITKRRFGYIYELPWYVIFGAPGSGKTTALTNSGLKFPLGDALGSNSVKGVGGTRNCNWWFTDEAILIDTAGRYTTQDDLNGTAKAGWEGFLGLLRKYRRSQPINGALVTLSIGDLLTRDPEAQREEIRAIRQRLSELDELLHARVPVYLLLTKADLLTGFVEFFDGFNKSDREQVWGTTFGLDESYKAANLPERFTEEFTLLQQRVDAMLIERLQQEPNPELRGRIFRFPAELASLKERLHEVTTELCSGSKLVEAPLLRGIYFASGTQREETAATPRMRRSYFLSRLFKEVIFGEASLVARDKRLSGRQ
Transmembrane helix 435-457 LLFRRAAYASAVLLLAMVLTSWT
Outside 458-1158 ATYFQNTAALAGAERRIDAYEQLVRGVPVRDVSDADFLRILPALDNLAAVTTDFSRTRVWPVSLGLDQEGKVASRQREAYQRALNTLLLPRMLVQLQKEMTETTDVNRTFDALKLYGMLGGLGPVNVDFASLEAEDMFTRLYPDEGRAAARKALIAHAGAMARGALPPIELDKTLIAKAREIIRSQNIASRAYEILAEHRASRALPGWSPAGALGPLGEQAFERTSKAPLGESIPGLFSATGYRTVVLPEISEAAREALDEQWVRGLPNPAGVTVDTVAQATLQLYFDKFDQRWSTLLTDIRVKPSQTISDAAETTRILAGKPGPVETIANSIAAATDLRGPSAEIASAAGDSLTDATTALSSAANAPDPFGPLREALKAPAGQSPLPDQSKQTPRSGIGQLEPILQKVQEQLSRATTSTAEVAKVFDVDSQLTDANQDLLQQARKLPAPVDTWMAGLAADVGSLAVKSARSRISDAWAAEGAGFCSNVVAGRYPFDRKSARDVAMSDFVRLFGPQGLFKTFFKEKLEAFVDTSASPWGWKGTFGAASIPSAAIAQFENADKINRAFFPAGSKDPLITINVKPVSLTDAASAVMLEIEGERVVYFHGPIQSKSISWPSAEASNTSRIAFQPGGWQQAMTENGDWSPFRLFDDANVTIQGDDLFRAKFEQDGRAAEFDVQFGSVLNPFRLEALGAFSCPAQF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9936 - -


  Protein sequence: 1159 a.a.    

>T6CP015727 NC_021911:c494624-491148 [Rhizobium etli bv. mimosae str. Mim1] [TssM]
MNPISYFYTLRSYVEAYAGLLGRRFISIIWVAAICVVIWFYGYLAAYGDFKPLASATARLTLIGVVIAAWLAYLLFTVIR
DGRRDKQLVEGIERDAEAEAAASQQAEVGEIHSRLKEALQLLRRITKRRFGYIYELPWYVIFGAPGSGKTTALTNSGLKF
PLGDALGSNSVKGVGGTRNCNWWFTDEAILIDTAGRYTTQDDLNGTAKAGWEGFLGLLRKYRRSQPINGALVTLSIGDLL
TRDPEAQREEIRAIRQRLSELDELLHARVPVYLLLTKADLLTGFVEFFDGFNKSDREQVWGTTFGLDESYKAANLPERFT
EEFTLLQQRVDAMLIERLQQEPNPELRGRIFRFPAELASLKERLHEVTTELCSGSKLVEAPLLRGIYFASGTQREETAAT
PRMRRSYFLSRLFKEVIFGEASLVARDKRLSGRQLLFRRAAYASAVLLLAMVLTSWTATYFQNTAALAGAERRIDAYEQL
VRGVPVRDVSDADFLRILPALDNLAAVTTDFSRTRVWPVSLGLDQEGKVASRQREAYQRALNTLLLPRMLVQLQKEMTET
TDVNRTFDALKLYGMLGGLGPVNVDFASLEAEDMFTRLYPDEGRAAARKALIAHAGAMARGALPPIELDKTLIAKAREII
RSQNIASRAYEILAEHRASRALPGWSPAGALGPLGEQAFERTSKAPLGESIPGLFSATGYRTVVLPEISEAAREALDEQW
VRGLPNPAGVTVDTVAQATLQLYFDKFDQRWSTLLTDIRVKPSQTISDAAETTRILAGKPGPVETIANSIAAATDLRGPS
AEIASAAGDSLTDATTALSSAANAPDPFGPLREALKAPAGQSPLPDQSKQTPRSGIGQLEPILQKVQEQLSRATTSTAEV
AKVFDVDSQLTDANQDLLQQARKLPAPVDTWMAGLAADVGSLAVKSARSRISDAWAAEGAGFCSNVVAGRYPFDRKSARD
VAMSDFVRLFGPQGLFKTFFKEKLEAFVDTSASPWGWKGTFGAASIPSAAIAQFENADKINRAFFPAGSKDPLITINVKP
VSLTDAASAVMLEIEGERVVYFHGPIQSKSISWPSAEASNTSRIAFQPGGWQQAMTENGDWSPFRLFDDANVTIQGDDLF
RAKFEQDGRAAEFDVQFGSVLNPFRLEALGAFSCPAQF*

  Nucleotide sequence: 3477 bp    

>T6CP015727 NC_021911:c494624-491148 [Rhizobium etli bv. mimosae str. Mim1] [TssM]
ATGAATCCCATAAGTTATTTCTATACGCTGCGTTCTTATGTGGAGGCCTATGCCGGCCTTCTCGGCCGCCGCTTCATCTC
GATCATATGGGTGGCGGCGATCTGCGTCGTCATCTGGTTCTACGGCTATCTCGCCGCCTATGGCGACTTTAAGCCGCTGG
CGAGCGCCACGGCGCGGCTGACGCTGATCGGCGTCGTCATCGCCGCCTGGCTCGCCTACCTCCTCTTCACCGTGATCCGC
GACGGCCGCCGCGACAAGCAGCTCGTCGAAGGGATCGAGCGTGACGCCGAGGCCGAAGCGGCGGCCAGCCAGCAGGCTGA
GGTCGGCGAGATCCACAGCCGGCTGAAGGAGGCGTTGCAGCTCCTGCGCCGCATCACCAAGCGACGCTTCGGCTATATTT
ACGAGCTGCCCTGGTACGTGATCTTCGGCGCGCCGGGCTCCGGCAAGACGACGGCGCTCACCAATTCCGGCCTCAAATTC
CCGCTCGGCGACGCACTCGGCAGCAATTCGGTGAAGGGCGTCGGCGGCACGCGCAACTGCAATTGGTGGTTCACCGACGA
GGCCATACTCATTGATACCGCCGGCCGCTACACCACCCAGGACGATCTGAACGGCACCGCCAAGGCCGGCTGGGAGGGCT
TTCTCGGCCTCCTCAGAAAATACCGCCGGTCGCAGCCGATCAACGGCGCGCTGGTGACGCTATCGATCGGCGACCTGCTG
ACACGCGATCCCGAAGCTCAGCGCGAGGAGATCAGAGCGATCCGCCAGAGGCTGTCCGAGCTCGACGAACTCCTGCATGC
ACGCGTGCCGGTCTATCTGCTCCTGACCAAGGCCGATCTCCTGACCGGCTTCGTCGAATTCTTCGACGGCTTCAACAAGA
GCGACCGCGAACAGGTCTGGGGCACGACCTTCGGGCTGGACGAAAGCTATAAGGCGGCCAACCTGCCGGAACGCTTCACA
GAGGAATTCACCCTGCTGCAGCAACGCGTCGACGCCATGCTGATCGAGCGCCTGCAGCAGGAGCCCAATCCGGAGCTGCG
CGGCCGCATCTTCCGCTTCCCCGCCGAGCTCGCCTCGCTGAAGGAGCGCCTGCATGAGGTGACGACGGAGCTTTGCTCGG
GCTCGAAACTGGTGGAGGCGCCGCTCCTGCGCGGCATCTATTTCGCCTCCGGCACCCAGAGGGAGGAGACCGCCGCCACG
CCGCGCATGCGGCGCAGCTATTTCCTCTCGCGCCTCTTCAAGGAGGTGATCTTCGGCGAGGCCTCGCTGGTTGCCCGCGA
CAAGCGGCTTTCCGGCCGACAGCTGCTCTTCCGCCGCGCGGCTTACGCCAGCGCGGTCCTGCTGCTGGCCATGGTGCTGA
CCAGCTGGACGGCCACCTATTTCCAGAACACCGCGGCGCTTGCGGGTGCGGAAAGACGCATCGACGCCTATGAGCAGCTG
GTGCGCGGCGTGCCGGTGCGCGACGTCTCAGATGCGGATTTCCTGCGCATTCTGCCGGCGCTCGATAATCTTGCTGCCGT
CACTACCGATTTTTCGAGGACGCGCGTCTGGCCGGTGAGCCTCGGCCTCGATCAGGAAGGCAAGGTCGCGAGCCGCCAGC
GCGAAGCCTATCAGCGGGCGCTGAACACGCTGCTCCTGCCCCGCATGCTGGTGCAGCTCCAGAAGGAGATGACCGAGACG
ACCGACGTCAACAGAACCTTCGACGCGCTGAAGCTCTACGGCATGCTCGGCGGGCTCGGCCCTGTGAATGTGGATTTCGC
CTCTTTGGAAGCGGAAGACATGTTCACCAGGCTCTATCCCGACGAAGGCCGGGCCGCTGCGCGCAAGGCGCTGATCGCCC
ATGCGGGAGCCATGGCGCGCGGCGCGCTGCCGCCCATCGAGCTCGACAAGACGCTGATCGCCAAGGCACGCGAGATTATC
CGCTCGCAGAACATCGCCAGCCGCGCCTATGAGATCCTGGCGGAACACCGCGCTTCCCGCGCCCTTCCCGGCTGGAGTCC
GGCCGGTGCGCTCGGTCCGCTCGGCGAACAGGCATTCGAGCGGACCTCCAAGGCGCCGCTTGGCGAGAGCATCCCCGGCC
TCTTTTCCGCGACCGGCTACCGGACCGTCGTTCTGCCGGAGATCAGCGAGGCCGCGCGCGAGGCACTCGACGAGCAGTGG
GTGCGCGGCTTACCCAATCCCGCCGGTGTCACGGTCGACACCGTCGCCCAGGCCACACTTCAGCTTTACTTCGACAAGTT
CGATCAACGCTGGTCGACCCTGTTGACCGATATCAGGGTCAAACCTTCGCAGACGATCAGCGACGCGGCTGAGACCACTC
GCATCCTCGCCGGCAAGCCCGGTCCCGTCGAGACGATCGCGAATTCGATCGCCGCGGCCACCGATCTGCGCGGCCCCAGC
GCCGAGATCGCCTCGGCTGCCGGAGACAGTTTGACCGACGCAACCACGGCGCTGAGCAGTGCCGCCAATGCGCCCGATCC
CTTCGGCCCGCTGCGAGAGGCGTTGAAGGCGCCGGCCGGCCAGTCGCCGCTCCCCGACCAATCGAAGCAGACGCCCCGCT
CGGGGATCGGACAGCTGGAACCGATCCTGCAGAAAGTACAGGAGCAGCTTTCGCGGGCGACGACCTCTACCGCTGAAGTG
GCAAAAGTCTTCGACGTCGATAGCCAGTTGACGGACGCCAACCAGGACTTGCTGCAGCAAGCCCGGAAGTTGCCGGCGCC
AGTCGATACATGGATGGCGGGGCTCGCCGCCGATGTCGGTTCGCTGGCGGTCAAATCCGCTCGTTCGCGCATCAGCGATG
CCTGGGCCGCGGAGGGTGCCGGCTTCTGCTCGAATGTCGTGGCCGGCCGTTATCCCTTCGATCGAAAATCGGCGCGCGAC
GTGGCGATGAGCGACTTTGTCAGGCTGTTCGGACCCCAGGGGCTGTTCAAGACCTTCTTCAAGGAAAAGCTTGAGGCCTT
CGTCGATACCAGCGCTTCGCCATGGGGCTGGAAGGGCACCTTCGGGGCGGCGAGCATTCCGAGCGCGGCGATCGCCCAGT
TCGAGAATGCCGACAAGATCAACCGCGCCTTCTTCCCAGCCGGCAGCAAGGACCCGTTGATCACCATCAACGTCAAGCCG
GTGTCGCTGACCGATGCCGCCAGCGCCGTGATGCTGGAGATCGAGGGCGAACGCGTCGTTTATTTCCATGGACCGATTCA
GTCGAAATCGATCAGCTGGCCATCGGCGGAAGCCAGCAACACCTCGCGGATCGCCTTCCAGCCGGGCGGCTGGCAGCAGG
CCATGACCGAAAACGGCGACTGGTCGCCCTTCCGCCTCTTCGACGATGCCAATGTCACGATCCAAGGCGACGATCTTTTC
CGGGCGAAATTTGAGCAGGATGGCCGGGCGGCGGAGTTCGACGTGCAGTTCGGTTCGGTGCTCAATCCATTCCGGCTGGA
GGCGCTCGGCGCCTTCTCATGCCCGGCGCAATTCTGA