Detailed information of component protein


Summary

Component ID T6CP002760
Component protein type TssF
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (630 a.a.); Nucleotide sequence (1890 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30545
Coordinate (Strand) 671060..672949 (+)
NCBI ID WP_012405335.1
RefSeq NC_010625
Uniprot ID B2JW49_PARP8
KEGG ID bph:Bphy_6119
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 3.8e-218 1..626
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-629 MDPRLLEYYNHELIYMRELASEFGRHHPKIGARLGMQAGEIGDPYVERLLESFCFMAARMQIKIDAEFPKFTGRLLEVIYPNYVAPTPSISVARIYPSPKEGNLNRGFQLPRGTMFKARKPAGEKTLCQFRSGLDVTLYPLGISEAKLTAIPPDVVGLDRYIPPDRQVRGALRIRFRTTNGSRISDLQGLNHLPIYLAGDEQVASHLFELLHAANVALVIGEPGQFGSGDKPLSVVTSNPVAHEGIGIGEGLLPLTWSKFHGHNLLHEYFACPSRFYFFALTGLAQGFSRINGPEVEVIVLLDQPTERLANLVDASRFALFCTPVVNLFEKRTDQIEIPQGESEVRLVPSKLEPLDYEVFAIQKLCGQKTSNSAELEFRPLFQTLNNDEGNYGRYFSSRRERRLASDNSRRYGTRTPYIGTEMFVTLVDQTEAPYNGDIRYLSADAWVTNRDLPLLVPRNGVDDLDGNDEDSIQSIGLIRPPSPPRPPYAEREMAWRLIRQLNFNYLPFSDMDHREGGQGLRDLLRLFLASDDTERQRQVEALIGVKTEPVNDMLPGNGPLVFGRGIECVLTVDETGFSGVSPYLLGLILEHYMARHVSINSFTQTELHSMQRGLVARWPVRMGTRGVA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9964 - -


  Protein sequence: 630 a.a.    

>T6CP002760 NC_010625:671060-672949 [Paraburkholderia phymatum STM815] [TssF]
MDPRLLEYYNHELIYMRELASEFGRHHPKIGARLGMQAGEIGDPYVERLLESFCFMAARMQIKIDAEFPKFTGRLLEVIY
PNYVAPTPSISVARIYPSPKEGNLNRGFQLPRGTMFKARKPAGEKTLCQFRSGLDVTLYPLGISEAKLTAIPPDVVGLDR
YIPPDRQVRGALRIRFRTTNGSRISDLQGLNHLPIYLAGDEQVASHLFELLHAANVALVIGEPGQFGSGDKPLSVVTSNP
VAHEGIGIGEGLLPLTWSKFHGHNLLHEYFACPSRFYFFALTGLAQGFSRINGPEVEVIVLLDQPTERLANLVDASRFAL
FCTPVVNLFEKRTDQIEIPQGESEVRLVPSKLEPLDYEVFAIQKLCGQKTSNSAELEFRPLFQTLNNDEGNYGRYFSSRR
ERRLASDNSRRYGTRTPYIGTEMFVTLVDQTEAPYNGDIRYLSADAWVTNRDLPLLVPRNGVDDLDGNDEDSIQSIGLIR
PPSPPRPPYAEREMAWRLIRQLNFNYLPFSDMDHREGGQGLRDLLRLFLASDDTERQRQVEALIGVKTEPVNDMLPGNGP
LVFGRGIECVLTVDETGFSGVSPYLLGLILEHYMARHVSINSFTQTELHSMQRGLVARWPVRMGTRGVA*

  Nucleotide sequence: 1890 bp    

>T6CP002760 NC_010625:671060-672949 [Paraburkholderia phymatum STM815] [TssF]
ATGGATCCACGACTGCTCGAATATTACAACCACGAATTGATCTATATGCGGGAGCTTGCCAGCGAATTCGGCCGGCACCA
CCCCAAGATCGGGGCGCGTCTGGGCATGCAGGCGGGTGAGATCGGCGACCCCTATGTCGAACGGCTCCTCGAGTCGTTCT
GCTTCATGGCGGCCCGTATGCAGATCAAGATCGACGCGGAGTTCCCGAAGTTTACCGGGCGGCTCCTTGAAGTCATCTAC
CCGAACTATGTCGCGCCGACTCCATCCATATCGGTCGCCCGCATCTATCCCAGTCCGAAGGAAGGGAACCTGAACCGCGG
TTTCCAGCTGCCACGTGGCACGATGTTCAAGGCACGCAAGCCAGCGGGCGAGAAAACCCTGTGCCAGTTCCGCAGCGGGC
TGGACGTCACGCTGTATCCGCTCGGCATTTCCGAAGCGAAACTCACGGCCATTCCACCCGATGTTGTCGGACTCGACCGG
TATATTCCGCCGGATCGCCAGGTCCGGGGCGCCTTGCGCATCCGGTTCCGTACCACGAACGGTTCGCGAATTTCGGATCT
GCAGGGCCTCAACCATTTGCCAATCTACCTTGCAGGTGACGAGCAGGTCGCTTCGCACCTGTTCGAACTGCTGCACGCGG
CCAATGTGGCTCTGGTCATTGGTGAACCAGGGCAGTTCGGTTCGGGCGACAAGCCGCTCTCGGTCGTCACGTCGAATCCT
GTTGCCCATGAGGGTATTGGTATTGGTGAAGGGCTGCTGCCGCTCACCTGGTCGAAATTCCATGGCCACAATCTTCTGCA
CGAGTACTTTGCGTGCCCGAGCCGGTTCTATTTCTTTGCGCTGACGGGTCTCGCGCAAGGATTTAGCCGGATCAACGGGC
CCGAAGTCGAGGTGATTGTGCTGCTGGATCAACCCACCGAACGGCTCGCGAACCTGGTGGATGCGTCGCGTTTTGCACTT
TTTTGCACTCCGGTCGTCAATCTGTTCGAGAAGCGGACCGACCAGATCGAAATCCCGCAGGGCGAGTCCGAGGTCCGGCT
CGTTCCATCGAAGCTGGAGCCGCTTGACTATGAGGTCTTCGCGATCCAGAAGCTGTGCGGCCAGAAGACGTCGAATTCGG
CTGAACTGGAGTTCCGGCCGCTGTTCCAGACGCTGAACAACGACGAGGGCAATTACGGGCGGTACTTTTCGAGCCGCCGC
GAGCGCCGCCTGGCGTCGGACAACAGTCGGCGATACGGCACGCGCACGCCCTACATCGGTACTGAGATGTTCGTCACGCT
GGTCGACCAGACCGAAGCGCCCTACAACGGGGACATCCGCTATCTGTCGGCCGATGCCTGGGTGACCAACCGCGACCTGC
CACTGCTCGTGCCTCGCAACGGCGTGGATGACCTCGACGGCAACGACGAGGACTCGATCCAGTCGATCGGTCTGATCCGG
CCACCCAGCCCTCCGCGGCCGCCTTACGCGGAACGCGAGATGGCTTGGCGCCTGATCCGGCAACTGAACTTCAACTACCT
GCCGTTCAGTGACATGGATCACCGCGAGGGTGGGCAGGGCCTGCGCGATCTGCTCAGACTGTTCCTTGCCAGCGATGATA
CCGAACGCCAGCGTCAGGTCGAAGCTCTCATTGGCGTGAAGACGGAACCCGTCAACGACATGCTGCCGGGCAACGGTCCA
CTCGTGTTCGGGCGTGGTATCGAGTGCGTGCTCACGGTCGATGAGACGGGCTTCTCGGGCGTCAGCCCCTACCTGCTCGG
CCTGATTCTCGAACACTACATGGCCCGGCACGTGTCGATCAATTCGTTCACGCAAACCGAGCTGCATTCAATGCAACGGG
GGTTGGTCGCCCGTTGGCCGGTACGCATGGGTACGCGTGGAGTGGCCTGA