SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000431
Component protein type	TssJ
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (189 a.a.); Nucleotide sequence (567 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01780
Coordinate (Strand)	393634..394200 (-)
NCBI ID	WP_011144755.1
RefSeq	NC_005126
Uniprot ID	Q7N9H0_PHOLL
KEGG ID	plu:plu0366
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.1e-32	59..179

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-9	MKRSGGNTS
Transmembrane helix	10-32	WFGSNTVWFVLLAALMLSGCSSA
Inside	33-188	WQATKKVGQVVWDPSTPVGQPANLPSTVAITLLAEPDINPNKNGEATPVEMHLLYLSEDSRLLAADYDQLASDKLDKALGKNYIDHQDYTLLPGQYKPLPVIPLEKNSRYIGVIVRYADINQSEWKKVIRVKDTGQQYHILVHIRANEVELRKEEQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9925	pos: 28-29. LSG-CS.	MKRSGGNTSWFGSNTVWFVLLAALMLSG

Protein sequence: 189 a.a.

						>T6CP000431 NC_005126:c394200-393634 [Photorhabdus laumondii subsp. laumondii TTO1] [TssJ]
MKRSGGNTSWFGSNTVWFVLLAALMLSGCSSAWQATKKVGQVVWDPSTPVGQPANLPSTVAITLLAEPDINPNKNGEATP
VEMHLLYLSEDSRLLAADYDQLASDKLDKALGKNYIDHQDYTLLPGQYKPLPVIPLEKNSRYIGVIVRYADINQSEWKKV
IRVKDTGQQYHILVHIRANEVELRKEEQ*					

Nucleotide sequence: 567 bp

						>T6CP000431 NC_005126:c394200-393634 [Photorhabdus laumondii subsp. laumondii TTO1] [TssJ]
ATGAAGCGCTCCGGCGGTAATACCTCATGGTTCGGTAGTAATACCGTATGGTTTGTATTGCTGGCAGCGCTGATGTTAAG
CGGTTGTAGCAGTGCATGGCAGGCAACGAAGAAAGTCGGACAGGTAGTCTGGGACCCATCAACTCCAGTTGGTCAACCTG
CGAACTTACCTTCCACCGTCGCGATCACTTTGCTGGCAGAACCTGATATTAACCCCAATAAAAATGGAGAAGCCACTCCG
GTTGAAATGCATCTGCTCTATCTCAGTGAAGATTCCCGTCTACTTGCCGCTGATTATGACCAACTGGCGAGTGACAAGCT
GGATAAAGCACTGGGGAAAAATTATATCGACCATCAGGATTACACCTTATTGCCGGGGCAGTACAAACCACTGCCAGTCA
TCCCGCTGGAAAAAAATAGCCGTTACATCGGCGTTATCGTTCGCTATGCCGATATTAATCAATCCGAATGGAAAAAGGTC
ATCCGTGTGAAAGACACAGGACAGCAATACCACATTCTGGTGCATATCAGAGCGAACGAAGTTGAACTTAGAAAAGAGGA
GCAATAA					