Detailed information NTG0999906

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABFU26_RS15990
• Genome accession: NZ_CP155935
• Coordinates: 3643140..3644873 (-)
• Length: 577 a.a.
• NCBI ID: WP_024939398.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. raphani strain Xcr-TH1B
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3635331..3648970	3643140..3644873	within	0

Gene organization within MGE regions

Location: 3635331..3648970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU26_RS15950 (ABFU26_15905)	-	3635331..3635633 (-)	303	WP_323475834.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABFU26_RS15955 (ABFU26_15910)	-	3635637..3636047 (-)	411	WP_029217117.1	CopG family ribbon-helix-helix protein	-
ABFU26_RS15960 (ABFU26_15915)	sucD	3636151..3637026 (-)	876	WP_047124757.1	succinate--CoA ligase subunit alpha	-
ABFU26_RS15965 (ABFU26_15920)	sucC	3637051..3638220 (-)	1170	WP_407460381.1	ADP-forming succinate--CoA ligase subunit beta	-
ABFU26_RS15970 (ABFU26_15925)	-	3638454..3640064 (+)	1611	WP_407460383.1	sensor histidine kinase	-
ABFU26_RS15975 (ABFU26_15930)	pilR	3640273..3641667 (+)	1395	WP_043921909.1	sigma-54 dependent transcriptional regulator	Regulator
ABFU26_RS15980 (ABFU26_15935)	-	3641865..3642221 (+)	357	WP_139328384.1	hypothetical protein	-
ABFU26_RS15985 (ABFU26_15940)	-	3642166..3642957 (+)	792	WP_202796265.1	toxin	-
ABFU26_RS15990 (ABFU26_15945)	pilB	3643140..3644873 (-)	1734	WP_024939398.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABFU26_RS15995 (ABFU26_15950)	-	3644915..3645328 (-)	414	WP_080689434.1	pilin	-
ABFU26_RS16000 (ABFU26_15955)	comP	3645425..3645856 (-)	432	WP_024939397.1	pilin	Machinery gene
ABFU26_RS16005 (ABFU26_15960)	pilC	3646204..3647463 (+)	1260	WP_024939396.1	type II secretion system F family protein	Machinery gene
ABFU26_RS16010 (ABFU26_15965)	-	3647470..3648333 (+)	864	WP_223646361.1	A24 family peptidase	-
ABFU26_RS16015 (ABFU26_15970)	coaE	3648347..3648970 (+)	624	WP_323486787.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62810.93 Da
• Isoelectric Point: 5.7377

>NTDB_id=999906 ABFU26_RS15990 WP_024939398.1 3643140..3644873(-) (pilB) [Xanthomonas campestris pv. raphani strain Xcr-TH1B]
MNAAISSNLVGITGIARRLVQDGALDEANARAAMDHAAQAKVPLPQWFAEKKLVTAAQLASANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=999906 ABFU26_RS15990 WP_024939398.1 3643140..3644873(-) (pilB) [Xanthomonas campestris pv. raphani strain Xcr-TH1B]
ATGAACGCTGCAATCAGCTCAAATCTAGTTGGAATTACCGGCATCGCGCGGCGTCTTGTTCAGGATGGTGCGTTAGATGA
AGCGAACGCACGTGCGGCAATGGATCATGCGGCCCAGGCTAAAGTTCCGTTGCCGCAATGGTTCGCCGAAAAGAAGCTCG
TAACAGCAGCGCAGCTCGCCTCTGCCAATGCAGTTGAGTTCGGTATGCCGCTGATGGACGTGTCGGTGTTCGACGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGCATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCACACCAATTTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCCTCGCTCGGC
GACGACGATGATGAAATGGGGGATCTGGACGTCTCTACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCACCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATTTCAACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCCTCGGTGACCCTGGTCATCGCGCAGCGTTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.986	98.44	0.551
pilB	Acinetobacter baylyi ADP1	55.458	98.44	0.546
pilB	Legionella pneumophila strain ERS1305867	51.389	99.827	0.513
pilB	Vibrio cholerae strain A1552	48	99.653	0.478
pilF	Neisseria gonorrhoeae MS11	48.239	98.44	0.475
pilB	Vibrio campbellii strain DS40M4	45.518	98.614	0.449
pilB	Vibrio parahaemolyticus RIMD 2210633	45.471	97.574	0.444