Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU19_RS16220 Genome accession   NZ_CP155928
Coordinates   3753818..3755551 (-) Length   577 a.a.
NCBI ID   WP_042594920.1    Uniprot ID   -
Organism   Xanthomonas campestris strain CFBP 13427     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3748818..3760551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU19_RS16210 (ABFU19_16180) - 3750177..3751787 (+) 1611 WP_115006667.1 PAS domain-containing sensor histidine kinase -
  ABFU19_RS16215 (ABFU19_16185) pilR 3751994..3753388 (+) 1395 WP_042594919.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU19_RS16220 (ABFU19_16190) pilB 3753818..3755551 (-) 1734 WP_042594920.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU19_RS16225 (ABFU19_16195) - 3755732..3756631 (+) 900 WP_080869819.1 glycosyltransferase family 2 protein -
  ABFU19_RS16230 (ABFU19_16200) - 3756624..3757790 (+) 1167 WP_119959176.1 glycosyltransferase -
  ABFU19_RS16235 (ABFU19_16205) - 3757782..3758699 (-) 918 WP_119959175.1 lysylphosphatidylglycerol synthase domain-containing protein -
  ABFU19_RS16240 (ABFU19_16210) - 3758696..3759532 (-) 837 WP_183110986.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62722.88 Da        Isoelectric Point: 5.7626

>NTDB_id=999779 ABFU19_RS16220 WP_042594920.1 3753818..3755551(-) (pilB) [Xanthomonas campestris strain CFBP 13427]
MTASLTTNLVGITGIARRLVQDGALDEAGARTAMDQAGAARVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=999779 ABFU19_RS16220 WP_042594920.1 3753818..3755551(-) (pilB) [Xanthomonas campestris strain CFBP 13427]
ATGACCGCATCGCTAACTACAAACTTGGTTGGTATTACGGGCATCGCTCGTCGATTGGTTCAGGACGGTGCGCTGGATGA
AGCCGGCGCGCGTACCGCCATGGATCAAGCCGGGGCGGCGAGGGTTCCGTTGCCACAATGGTTTGCAGAGAAGAAGCTGG
TGACTGCATCCCAGCTCGCCGCTGCAAATGCGGTTGAGTTCGGTATGCCGCTGATGGACGTGTCTGTCTTCGATGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTTCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTTGATGAGGACCAGATCCGTCGCACACTGGAGCAGTGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTGGACGTCTCCACTGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCTCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATTGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAACTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACGTCAAGCGGGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCCTCGGTGACCCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCA
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

98.44

0.555
  pilB Acinetobacter baylyi ADP1

55.106

98.44

0.542
  pilB Legionella pneumophila strain ERS1305867

51.562

99.827

0.515
  pilB Vibrio cholerae strain A1552

48.532

100

0.487
  pilF Neisseria gonorrhoeae MS11

48.239

98.44

0.475
  pilB Vibrio campbellii strain DS40M4

45.87

98.614

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.574

0.449

Multiple sequence alignment