Detailed information NTG0996285

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLNCDO184_RS11595
• Genome accession: NZ_CP155124
• Coordinates: 2244118..2244564 (-)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: A0AAC9R3D6
• Organism: Lactococcus lactis subsp. lactis strain NCDO184
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2239118..2249564

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO184_RS11560 (LLNCDO184_11550)	-	2239235..2239612 (-)	378	WP_063283703.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLNCDO184_RS11565 (LLNCDO184_11555)	-	2239817..2240683 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
LLNCDO184_RS11570 (LLNCDO184_11560)	-	2240722..2241531 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LLNCDO184_RS11575 (LLNCDO184_11565)	-	2241524..2242261 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
LLNCDO184_RS11580 (LLNCDO184_11570)	-	2242438..2243280 (-)	843	WP_063283655.1	metal ABC transporter substrate-binding protein	-
LLNCDO184_RS11585 (LLNCDO184_11575)	-	2243277..2243714 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LLNCDO184_RS11590 (LLNCDO184_11580)	comGG	2243795..2244079 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLNCDO184_RS11595 (LLNCDO184_11585)	comGF	2244118..2244564 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLNCDO184_RS11600 (LLNCDO184_11590)	comGE	2244527..2244823 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLNCDO184_RS11605 (LLNCDO184_11595)	comGD	2244795..2245193 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLNCDO184_RS11610 (LLNCDO184_11600)	comGC	2245186..2245569 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
LLNCDO184_RS11615 (LLNCDO184_11605)	comGB	2245583..2246656 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLNCDO184_RS11620 (LLNCDO184_11610)	comGA	2246550..2247488 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=996285 LLNCDO184_RS11595 WP_031296844.1 2244118..2244564(-) (comGF) [Lactococcus lactis subsp. lactis strain NCDO184]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=996285 LLNCDO184_RS11595 WP_031296844.1 2244118..2244564(-) (comGF) [Lactococcus lactis subsp. lactis strain NCDO184]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385