Detailed information NTG0994626

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: AAFU62_RS00675
• Genome accession: NZ_CP154891
• Coordinates: 104888..105322 (+)
• Length: 144 a.a.
• NCBI ID: WP_129321637.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain 318231
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99888..110322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFU62_RS00645 (AAFU62_00645)	-	101557..101922 (+)	366	WP_002986560.1	DUF1033 family protein	-
AAFU62_RS00650 (AAFU62_00650)	comYA	102015..102953 (+)	939	WP_063812149.1	competence type IV pilus ATPase ComGA	Machinery gene
AAFU62_RS00655 (AAFU62_00655)	comYB	102889..103923 (+)	1035	WP_233436572.1	competence type IV pilus assembly protein ComGB	Machinery gene
AAFU62_RS00660 (AAFU62_00660)	comYC	103925..104251 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
AAFU62_RS00665 (AAFU62_00665)	comGD	104226..104654 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
AAFU62_RS00670 (AAFU62_00670)	comGE	104611..104895 (+)	285	WP_032465969.1	competence type IV pilus minor pilin ComGE	-
AAFU62_RS00675 (AAFU62_00675)	comYF	104888..105322 (+)	435	WP_129321637.1	competence type IV pilus minor pilin ComGF	Machinery gene
AAFU62_RS00680 (AAFU62_00680)	comGG	105306..105632 (+)	327	WP_129321638.1	competence type IV pilus minor pilin ComGG	-
AAFU62_RS00685 (AAFU62_00685)	comYH	105730..106683 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
AAFU62_RS00690 (AAFU62_00690)	-	106742..107938 (+)	1197	WP_345866088.1	acetate kinase	-
AAFU62_RS00695 (AAFU62_00695)	-	108125..108433 (+)	309	Protein_88	hypothetical protein	-
AAFU62_RS00700 (AAFU62_00700)	proC	108516..109286 (-)	771	WP_136018957.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16587.23 Da
• Isoelectric Point: 10.3584

>NTDB_id=994626 AAFU62_RS00675 WP_129321637.1 104888..105322(+) (comYF) [Streptococcus pyogenes strain 318231]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQG

Nucleotide

• Length: 435 bp

>NTDB_id=994626 AAFU62_RS00675 WP_129321637.1 104888..105322(+) (comYF) [Streptococcus pyogenes strain 318231]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGGCTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.825	95.139	0.493
comYF	Streptococcus mutans UA159	51.095	95.139	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.667	93.75	0.438
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396