Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   AAFU62_RS00685 Genome accession   NZ_CP154891
Coordinates   105730..106683 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain 318231     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100730..111683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFU62_RS00645 (AAFU62_00645) - 101557..101922 (+) 366 WP_002986560.1 DUF1033 family protein -
  AAFU62_RS00650 (AAFU62_00650) comYA 102015..102953 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  AAFU62_RS00655 (AAFU62_00655) comYB 102889..103923 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAFU62_RS00660 (AAFU62_00660) comYC 103925..104251 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  AAFU62_RS00665 (AAFU62_00665) comGD 104226..104654 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  AAFU62_RS00670 (AAFU62_00670) comGE 104611..104895 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE -
  AAFU62_RS00675 (AAFU62_00675) comYF 104888..105322 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAFU62_RS00680 (AAFU62_00680) comGG 105306..105632 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  AAFU62_RS00685 (AAFU62_00685) comYH 105730..106683 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  AAFU62_RS00690 (AAFU62_00690) - 106742..107938 (+) 1197 WP_345866088.1 acetate kinase -
  AAFU62_RS00695 (AAFU62_00695) - 108125..108433 (+) 309 Protein_88 hypothetical protein -
  AAFU62_RS00700 (AAFU62_00700) proC 108516..109286 (-) 771 WP_136018957.1 pyrroline-5-carboxylate reductase -
  AAFU62_RS00705 (AAFU62_00705) pepA 109334..110401 (-) 1068 WP_136018958.1 glutamyl aminopeptidase -
  AAFU62_RS00710 (AAFU62_00710) - 110866..111150 (+) 285 WP_002992749.1 DUF4651 domain-containing protein -
  AAFU62_RS00715 (AAFU62_00715) - 111147..111464 (+) 318 WP_011285355.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=994627 AAFU62_RS00685 WP_002987790.1 105730..106683(+) (comYH) [Streptococcus pyogenes strain 318231]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=994627 AAFU62_RS00685 WP_002987790.1 105730..106683(+) (comYH) [Streptococcus pyogenes strain 318231]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACG
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTTAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAAATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656

Multiple sequence alignment