Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   AAFU62_RS00655 Genome accession   NZ_CP154891
Coordinates   102889..103923 (+) Length   344 a.a.
NCBI ID   WP_233436572.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 318231     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97889..108923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFU62_RS00645 (AAFU62_00645) - 101557..101922 (+) 366 WP_002986560.1 DUF1033 family protein -
  AAFU62_RS00650 (AAFU62_00650) comYA 102015..102953 (+) 939 WP_063812149.1 competence type IV pilus ATPase ComGA Machinery gene
  AAFU62_RS00655 (AAFU62_00655) comYB 102889..103923 (+) 1035 WP_233436572.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAFU62_RS00660 (AAFU62_00660) comYC 103925..104251 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  AAFU62_RS00665 (AAFU62_00665) comGD 104226..104654 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  AAFU62_RS00670 (AAFU62_00670) comGE 104611..104895 (+) 285 WP_032465969.1 competence type IV pilus minor pilin ComGE -
  AAFU62_RS00675 (AAFU62_00675) comYF 104888..105322 (+) 435 WP_129321637.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAFU62_RS00680 (AAFU62_00680) comGG 105306..105632 (+) 327 WP_129321638.1 competence type IV pilus minor pilin ComGG -
  AAFU62_RS00685 (AAFU62_00685) comYH 105730..106683 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  AAFU62_RS00690 (AAFU62_00690) - 106742..107938 (+) 1197 WP_345866088.1 acetate kinase -
  AAFU62_RS00695 (AAFU62_00695) - 108125..108433 (+) 309 Protein_88 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39688.20 Da        Isoelectric Point: 9.7085

>NTDB_id=994624 AAFU62_RS00655 WP_233436572.1 102889..103923(+) (comYB) [Streptococcus pyogenes strain 318231]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIEITLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=994624 AAFU62_RS00655 WP_233436572.1 102889..103923(+) (comYB) [Streptococcus pyogenes strain 318231]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAATAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment