Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAG092_RS13885 Genome accession   NZ_CP154874
Coordinates   2871096..2872160 (+) Length   354 a.a.
NCBI ID   WP_110682985.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2866096..2877160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS13875 (AAG092_13805) torT 2867459..2868490 (+) 1032 WP_373387126.1 TMAO reductase system periplasmic protein TorT -
  AAG092_RS13880 (AAG092_13810) - 2868497..2870911 (-) 2415 WP_373387127.1 penicillin-binding protein 1A -
  AAG092_RS13885 (AAG092_13815) pilM 2871096..2872160 (+) 1065 WP_110682985.1 pilus assembly protein PilM Machinery gene
  AAG092_RS13890 (AAG092_13820) - 2872160..2872792 (+) 633 WP_373387128.1 PilN domain-containing protein -
  AAG092_RS13895 (AAG092_13825) pilO 2872789..2873415 (+) 627 WP_373387129.1 type 4a pilus biogenesis protein PilO -
  AAG092_RS13900 (AAG092_13830) pilP 2873415..2873938 (+) 524 Protein_2741 type 4a pilus biogenesis lipoprotein PilP -
  AAG092_RS13905 (AAG092_13835) pilQ 2873992..2876052 (+) 2061 WP_373387130.1 type IV pilus secretin PilQ Machinery gene
  AAG092_RS13910 (AAG092_13840) aroK 2876057..2876575 (+) 519 WP_110682990.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37832.16 Da        Isoelectric Point: 4.4203

>NTDB_id=994336 AAG092_RS13885 WP_110682985.1 2871096..2872160(+) (pilM) [Pseudomonas alcaligenes strain Med1]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKTGVRTAAV
AVAGSAVITKTIEMEAGLNDDELENQLKIEADQYIPYPLEEVAIDFEVQGPSARNPERVEVLLAACRKENVEVREAALGL
AGLTAKVVDVEAYALERAYGLLESQLGGSVDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALGGKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=994336 AAG092_RS13885 WP_110682985.1 2871096..2872160(+) (pilM) [Pseudomonas alcaligenes strain Med1]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTTCTGGGGATCGACATCAGTTCGACCTCGGTCAAGCTCCTTGAATT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCCTATGCCGTCGAGCCGCTCCCGCCGAATGCCGTTGTCGAGAAGAACA
TCGCCGAGCTTGAGGGGGTCGGTCAGGCTCTGTCTCGTGTCCTGGCCAAGGCCAAGACCGGAGTGCGTACTGCGGCGGTC
GCCGTGGCGGGCTCCGCGGTGATCACCAAGACCATCGAGATGGAAGCGGGTCTCAACGACGACGAGTTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATTCCTTATCCGCTGGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGCCCTTCGGCAC
GCAATCCCGAGCGGGTCGAGGTGCTCTTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTTCGCGAGGCGGCGCTGGGGCTG
GCTGGGCTGACGGCCAAGGTGGTCGATGTCGAGGCCTATGCGCTTGAGCGAGCCTACGGTTTGCTCGAATCCCAGCTCGG
CGGCAGTGTCGATGAGCTGACCGTGGCGGTCGTCGATATCGGCGCCACCATGACGACGCTCAGCGTGCTGCATAACGGGC
GAACCATCTACACCCGCGAGCAGCTCTTTGGCGGGCGTCAGCTGACCGAAGAAATACAGCGTCGCTACGGTCTCTCGGTC
GAGGAGGCTGGGCTCGCCAAGAAACAGGGCGGCCTGCCGGACGACTACGACAGCGAAGTGCTGCAGCCTTTCAAGGAGGC
GGTTGTGCAGCAGGTCTCGCGCTCCCTGCAGTTCTTCTTTGCCGCGGGTCAGTTCAACGATGTCGACTACATCCTGCTGG
CGGGTGGCACTGCATCCATTCCCGACCTGGATCGTCTGATCCAGCAGAAGATCGGCACTCCCACTCTGGTGGCCAACCCG
TTCGCCGACATGGCGCTGGGGGGTAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCGTCCTTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment