Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAIA72_RS06120 Genome accession   NZ_CP154858
Coordinates   1258453..1260165 (+) Length   570 a.a.
NCBI ID   WP_369602525.1    Uniprot ID   -
Organism   Hahella sp. SMD15-11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1253453..1265165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA72_RS06100 (AAIA72_06085) - 1253772..1254953 (-) 1182 WP_369602521.1 glycosyltransferase -
  AAIA72_RS06105 (AAIA72_06090) - 1254963..1255823 (-) 861 WP_369602522.1 glycosyltransferase family 2 protein -
  AAIA72_RS06110 (AAIA72_06095) - 1255816..1257675 (-) 1860 WP_369602523.1 hypothetical protein -
  AAIA72_RS06115 (AAIA72_06100) - 1257744..1258154 (-) 411 WP_369602524.1 pilin -
  AAIA72_RS06120 (AAIA72_06105) pilB 1258453..1260165 (+) 1713 WP_369602525.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAIA72_RS06125 (AAIA72_06110) - 1260405..1261387 (+) 983 Protein_1181 type II secretion system F family protein -
  AAIA72_RS06130 (AAIA72_06115) pilD 1261394..1262278 (+) 885 WP_369602526.1 A24 family peptidase Machinery gene
  AAIA72_RS06135 (AAIA72_06120) coaE 1262281..1262922 (+) 642 WP_369602527.1 dephospho-CoA kinase -
  AAIA72_RS06140 (AAIA72_06125) yacG 1262828..1263028 (+) 201 WP_369602528.1 DNA gyrase inhibitor YacG -
  AAIA72_RS06145 (AAIA72_06130) - 1263025..1263393 (+) 369 WP_369602529.1 CidA/LrgA family protein -
  AAIA72_RS06150 (AAIA72_06135) - 1263380..1264057 (+) 678 WP_369602530.1 LrgB family protein -
  AAIA72_RS06155 (AAIA72_06140) - 1264093..1265118 (+) 1026 WP_369602531.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63465.73 Da        Isoelectric Point: 5.3573

>NTDB_id=994126 AAIA72_RS06120 WP_369602525.1 1258453..1260165(+) (pilB) [Hahella sp. SMD15-11]
MNTPAKPLTGHARRLVQDGLLDEAAALRAQEESRKEGISIITYLVQKGLVKASTLATTLSNEFGVPLLDLDAFDWHHMPD
KVVDEKLIRKHHVLPLYVRGNRLFLAVSEPTNFQALDEIKFHTGMSTDAILAEEDKLARAIDRYLDQQEQPLGDMGDMDM
DDLDVEAVDEGADRDEATSPEQDDAPIVKYINKMLLDAIKTGASDIHFEPYEKMYRVRYRIDGILREVSKPSVKLANRLS
ARIKVMSQLDISERRIPQDGRIKLKLSKNKAIDFRVNTLPTLWGEKIVLRILDPSSAQLGIDALGYEEDQKKLYLDALAQ
PQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVNPKVGLDFAAALRSFLRQDPDVVMVGEI
RDLETASIAIKAAQTGHLVLSTLHTNSAAETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCPECKTPMEVPEKVLREEG
FTQEQIETGLTLYQPQGCDKCKDGYKGRVGVYEVVRITPEMQDLIMRDASSLELARKAQEQGFRNLRQSALMKVMQGVTS
LAEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=994126 AAIA72_RS06120 WP_369602525.1 1258453..1260165(+) (pilB) [Hahella sp. SMD15-11]
ATGAATACACCGGCCAAACCCCTGACGGGTCACGCGAGACGACTGGTACAGGACGGACTGCTGGATGAAGCAGCCGCCCT
GCGAGCCCAAGAGGAAAGCAGGAAGGAAGGCATTTCCATTATTACCTACCTCGTCCAGAAGGGGCTGGTCAAGGCCAGTA
CGCTGGCCACGACGCTTTCCAACGAGTTTGGCGTGCCCTTGCTGGATCTGGATGCCTTCGACTGGCACCACATGCCGGAC
AAGGTGGTGGATGAGAAGCTGATTCGCAAGCATCATGTCCTGCCCCTTTATGTGCGTGGCAATCGGCTGTTTCTGGCGGT
CTCCGAACCCACCAATTTCCAGGCACTGGACGAGATCAAATTCCACACCGGCATGAGCACGGACGCCATTCTGGCCGAAG
AAGACAAGCTTGCCCGGGCCATTGACCGCTATCTCGACCAGCAGGAACAGCCGCTCGGCGACATGGGCGACATGGACATG
GATGACCTGGATGTGGAGGCCGTGGATGAAGGTGCCGACCGCGACGAAGCCACCTCCCCTGAACAGGATGATGCGCCGAT
CGTCAAGTACATCAACAAGATGTTGCTGGATGCCATCAAGACCGGCGCATCGGATATTCACTTCGAGCCCTATGAAAAGA
TGTATCGGGTGCGCTACCGAATTGACGGTATTTTGCGGGAGGTTTCCAAACCCTCCGTCAAGCTCGCCAATCGCCTCTCG
GCACGGATCAAGGTCATGTCCCAGCTGGACATTTCCGAACGGCGCATTCCCCAGGATGGCCGGATCAAGCTCAAACTGTC
CAAGAACAAGGCCATTGACTTCCGGGTCAACACCCTACCGACCCTGTGGGGTGAAAAAATCGTGCTCCGGATACTGGATC
CTTCCAGCGCCCAGCTCGGTATTGATGCCTTGGGCTACGAGGAAGACCAGAAGAAGTTGTATCTGGATGCCCTGGCACAA
CCACAGGGCATGATTCTGGTTACCGGTCCCACCGGTTCCGGTAAAACGGTTTCTCTTTATACCGGTCTCAACATTCTGAA
CACGCCGGAGCGGAACATTTCCACGGCAGAGGACCCGGTCGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACC
CCAAGGTGGGGCTGGACTTTGCAGCAGCACTGCGCTCGTTCCTGCGACAGGATCCGGACGTGGTCATGGTGGGCGAGATC
CGGGACCTGGAAACTGCCTCCATCGCCATCAAGGCTGCCCAGACAGGTCACCTGGTACTCTCGACACTGCACACCAACAG
TGCTGCCGAAACCCTGACGCGCCTGCGCAACATGGGTGTACCCGCATTCAACATTGCGACCTCGGTGAGCCTGATCATTG
CCCAGCGCCTTGCCCGGCGGCTTTGCCCGGAGTGCAAGACCCCCATGGAGGTGCCGGAGAAGGTGCTGCGTGAGGAAGGG
TTCACACAGGAACAAATTGAAACAGGCCTGACGTTGTACCAGCCCCAAGGCTGTGATAAATGCAAGGATGGATACAAGGG
GCGCGTGGGTGTGTACGAAGTGGTACGCATCACCCCCGAGATGCAGGACCTCATCATGCGCGACGCCAGCTCTCTGGAAC
TGGCCCGAAAGGCTCAGGAGCAGGGTTTCCGCAACCTGCGCCAGTCCGCGCTGATGAAGGTCATGCAGGGTGTCACCAGC
CTCGCTGAGGCCAACCGTGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.42

100

0.605
  pilB Acinetobacter baumannii D1279779

60.816

98.947

0.602
  pilB Legionella pneumophila strain ERS1305867

55.888

99.825

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

54.659

97.895

0.535
  pilB Vibrio campbellii strain DS40M4

53.464

98.772

0.528
  pilB Vibrio cholerae strain A1552

55

94.737

0.521
  pilF Neisseria gonorrhoeae MS11

49.027

99.123

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.888

93.684

0.374
  pilF Thermus thermophilus HB27

36.637

99.123

0.363

Multiple sequence alignment