Detailed information NTG0993856

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AAEQ75_RS11100
• Genome accession: NZ_CP154631
• Coordinates: 2333259..2334323 (+)
• Length: 354 a.a.
• NCBI ID: WP_017678539.1
• Uniprot ID: A0A1G6YHQ2
• Organism: Pseudomonas sediminis strain I4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2328259..2339323

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEQ75_RS11085 (AAEQ75_11085)	-	2328432..2329700 (+)	1269	WP_256837170.1	malic enzyme-like NAD(P)-binding protein	-
AAEQ75_RS11090 (AAEQ75_11090)	-	2329812..2330651 (+)	840	Protein_2189	transposase	-
AAEQ75_RS11095 (AAEQ75_11095)	-	2330666..2333077 (-)	2412	WP_343348694.1	penicillin-binding protein 1A	-
AAEQ75_RS11100 (AAEQ75_11100)	pilM	2333259..2334323 (+)	1065	WP_017678539.1	pilus assembly protein PilM	Machinery gene
AAEQ75_RS11105 (AAEQ75_11105)	-	2334323..2334898 (+)	576	WP_143504517.1	PilN domain-containing protein	-
AAEQ75_RS11110 (AAEQ75_11110)	pilO	2334895..2335521 (+)	627	WP_143504516.1	type 4a pilus biogenesis protein PilO	-
AAEQ75_RS11115 (AAEQ75_11115)	pilP	2335518..2336042 (+)	525	WP_143504515.1	type 4a pilus biogenesis lipoprotein PilP	-
AAEQ75_RS11120 (AAEQ75_11120)	pilQ	2336097..2338187 (+)	2091	WP_343352368.1	type IV pilus secretin PilQ	Machinery gene
AAEQ75_RS11125 (AAEQ75_11125)	aroK	2338193..2338711 (+)	519	WP_343348699.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37829.19 Da
• Isoelectric Point: 4.4647

>NTDB_id=993856 AAEQ75_RS11100 WP_017678539.1 2333259..2334323(+) (pilM) [Pseudomonas sediminis strain I4]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYSVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=993856 AAEQ75_RS11100 WP_017678539.1 2333259..2334323(+) (pilM) [Pseudomonas sediminis strain I4]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCTTATTCAGTCGAGCCGCTTCCACCTAACGCAGTGGTAGAGAAGAACA
TCGCCGAACTGGAGGGGGTCGGTCAGGCGCTCTCGCGCGTGTTGGCCAAGGCCAAGAGCGGCGTCAAGACGGCCGCGGTT
GCTGTGGCTGGTTCGGCGGTCATCACCAAAACCATCGAAATGGAAGCCGGCCTTTCCGAGGATGACCTGGAAAACCAGCT
GAAGATCGAGGCTGACCAGTACATTCCTTATCCGTTGGAAGAGGTTGCCATCGATTTCGAAGTGCAGGGCCCGGCGCCGC
GTAATCCTGAGCGGGTTGAGGTGCTGCTGGCTGCATGCCGCAAGGAAAACGTGGAGGTCCGCGAAGCAGCCCTCGCGCTC
GCCGGGCTTACTGCGAAAGTGGTCGATGTCGAGGCCTATGCCCTCGAGCGCGCTTACGGTTTGCTGGAGGCGCAGCTGGG
TGGCGGTCACGATGAGTTGACCGTCGCGGTGGTGGATATCGGGGCGACGATGACCACGCTCAGTGTTCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGCTTTTCGGTGGCAAGCAACTGACCGAGGAAATCCAGCGCCGCTATGGCCTGTCGGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAAGC
TGTGGTTCAGCAGGTTTCGCGCTCGCTGCAGTTCTTCTTCGCCGCCGGGCAATTCAATGATGTCGACTACATCCTCCTGG
CTGGTGGTACCGCGTCCATTCCTGACCTCGATCGTCTGATCCAGCAGAAGATCGGTACGCAGACGTTAGTGGCCAATCCC
TTTGCCGATATGGCGCTGAGCAGCAAGGTCAATGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1G6YHQ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	44.915	100	0.449