Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHAS_RS20280 Genome accession   NZ_AP035801
Coordinates   4164400..4165785 (+) Length   461 a.a.
NCBI ID   WP_001025180.1    Uniprot ID   A0A0H2V435
Organism   Escherichia coli strain E. coli JCM:5491     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4159400..4170785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAS_RS20255 (ReJCM5491_38280) aroP 4159950..4161320 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  ACGHAS_RS20260 (ReJCM5491_38290) ampE 4161363..4162217 (-) 855 WP_000172013.1 beta-lactamase regulator AmpE -
  ACGHAS_RS20265 (ReJCM5491_38300) ampD 4162214..4162765 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACGHAS_RS20270 (ReJCM5491_38310) nadC 4162853..4163746 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGHAS_RS20275 (ReJCM5491_38320) pilA 4163950..4164390 (+) 441 WP_000360915.1 prepilin peptidase-dependent pilin Machinery gene
  ACGHAS_RS20280 (ReJCM5491_38330) pilB 4164400..4165785 (+) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  ACGHAS_RS20285 (ReJCM5491_38340) hofC 4165775..4166977 (+) 1203 WP_000157242.1 protein transport protein HofC -
  ACGHAS_RS20290 (ReJCM5491_38350) guaC 4167012..4168055 (-) 1044 WP_001217338.1 GMP reductase -
  ACGHAS_RS20295 - 4168211..4168255 (-) 45 WP_120795372.1 protein YacM -
  ACGHAS_RS20300 (ReJCM5491_38360) coaE 4168280..4168900 (+) 621 WP_001269525.1 dephospho-CoA kinase -
  ACGHAS_RS20305 (ReJCM5491_38370) zapD 4168900..4169643 (+) 744 WP_001194731.1 cell division protein ZapD -
  ACGHAS_RS20310 (ReJCM5491_38380) yacG 4169653..4169850 (+) 198 WP_000005044.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50602.20 Da        Isoelectric Point: 6.5396

>NTDB_id=99142 ACGHAS_RS20280 WP_001025180.1 4164400..4165785(+) (pilB) [Escherichia coli strain E. coli JCM:5491]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=99142 ACGHAS_RS20280 WP_001025180.1 4164400..4165785(+) (pilB) [Escherichia coli strain E. coli JCM:5491]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTAGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V435

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367

Multiple sequence alignment