Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAGF34_RS00645 Genome accession   NZ_CP152376
Coordinates   141308..143041 (+) Length   577 a.a.
NCBI ID   WP_342618709.1    Uniprot ID   -
Organism   Rhodoferax sp. GW822-FHT02A01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 136308..148041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGF34_RS00615 (AAGF34_00615) - 136534..137562 (+) 1029 WP_342618704.1 polyprenyl synthetase family protein -
  AAGF34_RS00625 (AAGF34_00625) rfbB 137742..138827 (+) 1086 WP_342618705.1 dTDP-glucose 4,6-dehydratase -
  AAGF34_RS00630 (AAGF34_00630) rfbD 138824..139732 (+) 909 WP_342618706.1 dTDP-4-dehydrorhamnose reductase -
  AAGF34_RS00635 (AAGF34_00635) rfbA 139743..140654 (+) 912 WP_342618707.1 glucose-1-phosphate thymidylyltransferase RfbA -
  AAGF34_RS00640 (AAGF34_00640) rfbC 140654..141196 (+) 543 WP_342618708.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  AAGF34_RS00645 (AAGF34_00645) pilB 141308..143041 (+) 1734 WP_342618709.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGF34_RS00650 (AAGF34_00650) pilC 143061..144284 (+) 1224 WP_342618710.1 type II secretion system F family protein Machinery gene
  AAGF34_RS00655 (AAGF34_00655) - 144284..145129 (+) 846 WP_342618711.1 A24 family peptidase -
  AAGF34_RS00660 (AAGF34_00660) coaE 145132..145740 (+) 609 WP_342618712.1 dephospho-CoA kinase -
  AAGF34_RS00665 (AAGF34_00665) zapD 145763..146518 (+) 756 WP_342618713.1 cell division protein ZapD -
  AAGF34_RS00670 (AAGF34_00670) - 146535..146738 (+) 204 WP_342618714.1 DNA gyrase inhibitor YacG -
  AAGF34_RS00675 (AAGF34_00675) - 146714..147199 (-) 486 WP_342618715.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62966.28 Da        Isoelectric Point: 6.0587

>NTDB_id=988734 AAGF34_RS00645 WP_342618709.1 141308..143041(+) (pilB) [Rhodoferax sp. GW822-FHT02A01]
MAAPVDSFLQDSSAIALPGLGRAMVAAGKLEQKAAEDIYRKSISNKTSFISELVGSGAVSASDLAHTLSQAFAAPLINLD
ALDPQRLPKGLLDNKTCQDYRLVVLSKRNNRLLVATADPSDQRAAEKIKFSTQMGVDWIIAEYDKLSKMVEANSVSATEA
VEDIIGGDFEFDENAVDTAVEQSQVTAEVEDAPVVRFLQKMLLDAFSMRASDLHFEPYEHSYRVRFRIDGQLREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLKAISRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARKLCPTCRKPADIPK
QALLDAGFTEDMLDGSWKPYKPVGCSSCNMGYKGRVGIYQVMPVSEEIQRIILADGSSLDIAKQAEREGVRSLRQSGLRK
VQTGHTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=988734 AAGF34_RS00645 WP_342618709.1 141308..143041(+) (pilB) [Rhodoferax sp. GW822-FHT02A01]
ATGGCCGCACCCGTAGATTCATTCCTGCAAGATTCGAGCGCAATAGCGCTGCCCGGCCTGGGGCGTGCCATGGTGGCCGC
CGGAAAGCTTGAACAAAAAGCCGCCGAGGACATTTACCGTAAGTCGATTTCCAACAAGACCAGCTTCATCTCCGAGCTGG
TGGGGTCGGGCGCCGTATCGGCCTCGGACCTGGCGCATACGCTGTCCCAGGCATTTGCCGCCCCTCTGATCAATCTGGAC
GCGCTAGACCCCCAGCGTTTGCCAAAGGGTCTGCTGGACAACAAGACCTGCCAGGACTATCGCCTGGTGGTCCTGAGCAA
GCGCAACAACCGCCTCCTGGTAGCTACAGCCGACCCGTCTGACCAGCGTGCGGCGGAAAAAATCAAGTTTTCCACCCAGA
TGGGGGTGGACTGGATCATCGCCGAATACGACAAGCTTTCGAAAATGGTGGAGGCCAATTCGGTCAGTGCTACCGAGGCC
GTGGAAGACATCATTGGCGGCGACTTCGAATTCGACGAAAACGCCGTCGATACCGCGGTGGAACAGTCGCAAGTCACAGC
CGAGGTGGAAGATGCGCCCGTGGTGCGCTTCTTGCAGAAAATGCTGCTGGACGCCTTCAGCATGCGGGCCTCGGACTTGC
ACTTCGAACCGTACGAACACAGTTACCGGGTGCGATTCCGTATCGACGGCCAGCTGCGAGAAATTGCCAGCCCCCCCATC
GCCATCAAGGACAAGCTTGCCTCCCGTATCAAGGTGATTTCCCGCATGGATATCTCCGAGAAGCGTGTACCGCAGGACGG
ACGCATGAAACTCAAGGTCGGCCCCGACCGGGTCATCGATTTCCGTGTCAGCACCCTGCCCACGCTGTTTGGCGAGAAGA
TCGTGATCCGTATCCTGGACCCCTCCAGCGCCAAACTGGGTATTGACGCCCTGGGCTATGAGCCCGAGGAAAAAGAACGC
CTGCTCAAGGCCATCAGCAGACCATACGGGATGATACTGGTCACCGGCCCCACTGGCTCAGGCAAGACCGTGTCCTTGTA
CACCTGCCTGAACCTGCTGAATCAACCGGGTGTCAATATCGCCACGGCGGAAGACCCCTCCGAAATCAACTTGCCTGGTG
TCAACCAGGTCAATGTGAATGAAAAAGCCGGACTGACGTTTGCCGCAGCCCTGAAGTCCTTTCTGCGCCAGGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGACCTGGAAACGGCAGACATCTCCATCAAGGCGGCGCAAACCGGCCACCTGGTCCT
GTCCACCCTGCACACCAACGACGCACCGACCACGCTCACACGTATGCGCAATATGGGCATTGCCCCGTTCAATATTGCCT
CCAGCGTGATCCTGATCACCGCCCAGCGTCTGGCCCGCAAACTGTGCCCCACCTGCCGCAAGCCAGCCGACATTCCCAAG
CAGGCCCTGCTGGACGCCGGCTTTACCGAAGACATGCTCGACGGTTCCTGGAAGCCCTACAAGCCAGTTGGCTGCTCAAG
CTGCAACATGGGTTACAAAGGCCGCGTGGGCATTTACCAGGTGATGCCGGTATCGGAGGAAATTCAGCGCATCATTCTCG
CGGATGGCAGTTCCCTCGACATTGCGAAGCAGGCCGAGCGGGAAGGGGTGCGATCCCTGAGACAATCGGGCCTGAGGAAA
GTGCAGACTGGCCATACCTCGCTTGAAGAGGTTCTGGCTGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.196

92.721

0.53
  pilB Acinetobacter baylyi ADP1

53.697

98.44

0.529
  pilF Neisseria gonorrhoeae MS11

52.135

97.4

0.508
  pilB Legionella pneumophila strain ERS1305867

50.441

98.267

0.496
  pilB Vibrio campbellii strain DS40M4

44.071

97.92

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

46.679

91.334

0.426
  pilB Vibrio cholerae strain A1552

45.269

93.414

0.423
  pilF Thermus thermophilus HB27

37.589

97.747

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.286

92.201

0.362

Multiple sequence alignment