Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHH29_RS05245 Genome accession   NZ_CP152048
Coordinates   992035..993162 (-) Length   375 a.a.
NCBI ID   WP_082136017.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0046     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987035..998162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH29_RS05210 (MHH29_05210) flgL 987218..988129 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  MHH29_RS05215 (MHH29_05215) flgK 988138..989658 (-) 1521 WP_058336981.1 flagellar hook-associated protein FlgK -
  MHH29_RS05220 (MHH29_05220) - 989674..990156 (-) 483 WP_007499977.1 flagellar protein FlgN -
  MHH29_RS05225 (MHH29_05225) flgM 990175..990438 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHH29_RS05230 (MHH29_05230) - 990518..990937 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  MHH29_RS05235 (MHH29_05235) comFC 990994..991680 (-) 687 WP_172793290.1 ComF family protein Machinery gene
  MHH29_RS05240 (MHH29_05240) - 991677..991970 (-) 294 WP_008348267.1 competence protein ComFB -
  MHH29_RS05245 (MHH29_05245) comFA 992035..993162 (-) 1128 WP_082136017.1 DEAD/DEAH box helicase Machinery gene
  MHH29_RS05250 (MHH29_05250) - 993534..994376 (-) 843 WP_024719242.1 DegV family protein -
  MHH29_RS05255 (MHH29_05255) degU 994588..995277 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHH29_RS05260 (MHH29_05260) degS 995342..996517 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  MHH29_RS05265 (MHH29_05265) - 996739..997407 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42862.80 Da        Isoelectric Point: 10.4727

>NTDB_id=986785 MHH29_RS05245 WP_082136017.1 992035..993162(-) (comFA) [Bacillus sp. FSL K6-0046]
MLGKVSECGFLYEWTGPQKKETRRVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVSKSMKKTGVRIYLSATPSKKMQKDVAHRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=986785 MHH29_RS05245 WP_082136017.1 992035..993162(-) (comFA) [Bacillus sp. FSL K6-0046]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACGAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTGGAGCTCGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGGTTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTCAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCATAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATATGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTATACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-335)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae D39

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae R6

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae TIGR4

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis NCTC 12261

40.113

94.4

0.379
  comFA/cflA Streptococcus mitis SK321

38.983

94.4

0.368
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365

Multiple sequence alignment