Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHI46_RS16780 Genome accession   NZ_CP152042
Coordinates   3215404..3216549 (-) Length   381 a.a.
NCBI ID   WP_342502963.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3210404..3221549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS16745 (MHI46_16745) flgL 3210604..3211512 (-) 909 WP_039181799.1 flagellar hook-associated protein FlgL -
  MHI46_RS16750 (MHI46_16750) flgK 3211522..3213042 (-) 1521 WP_251181035.1 flagellar hook-associated protein FlgK -
  MHI46_RS16755 (MHI46_16755) - 3213058..3213540 (-) 483 WP_024423619.1 flagellar protein FlgN -
  MHI46_RS16760 (MHI46_16760) flgM 3213559..3213822 (-) 264 WP_024423620.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHI46_RS16765 (MHI46_16765) - 3213902..3214321 (-) 420 WP_039181808.1 TIGR03826 family flagellar region protein -
  MHI46_RS16770 (MHI46_16770) comFC 3214378..3215034 (-) 657 WP_251181034.1 ComF family protein Machinery gene
  MHI46_RS16775 (MHI46_16775) - 3215061..3215354 (-) 294 WP_058837538.1 competence protein ComFB -
  MHI46_RS16780 (MHI46_16780) comFA 3215404..3216549 (-) 1146 WP_342502963.1 DEAD/DEAH box helicase Machinery gene
  MHI46_RS16785 (MHI46_16785) - 3216922..3217764 (-) 843 WP_039181815.1 DegV family protein -
  MHI46_RS16790 (MHI46_16790) degU 3217981..3218670 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHI46_RS16795 (MHI46_16795) degS 3218735..3219910 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  MHI46_RS16800 (MHI46_16800) - 3220132..3220800 (+) 669 WP_251181032.1 YigZ family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 43466.75 Da        Isoelectric Point: 10.3557

>NTDB_id=986509 MHI46_RS16780 WP_342502963.1 3215404..3216549(-) (comFA) [Bacillus sp. FSL K6-1000]
MMRKATECGFLYEWTGPQMVETYRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYALNQGM
SVCIATPRTDVVLELEPRLRKAFQGMTIAVLYGGSSQRFQIAPLVIATTHQLMRYKNAFDVVIVDEVDAFPYSMDERLQF
AVLKAMRKNGGVRIYLSATPSKKMTREVSSGKLEAIKIPLRFHKQPLPVPTFHWIGHWKKKLKKNQLPRKVMNWMQRHIA
NKRRVLLFVPSIATMKKVTKILREHSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGSESTI
FTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIVKMNDTAANEFSEKQCGFN

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=986509 MHI46_RS16780 WP_342502963.1 3215404..3216549(-) (comFA) [Bacillus sp. FSL K6-1000]
ATGATGAGAAAAGCAACTGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATATCGAGCTGAATT
AACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATCAAAAACAAATTTGATC
TACTTGTTTGGGCGGTTTGCGGGGCAGGGAAAACGGAGGTGCTTTTTCACGGAATCGAATATGCGTTAAATCAAGGAATG
AGTGTCTGTATTGCGACCCCTAGAACAGATGTTGTACTTGAACTTGAACCACGGCTTAGAAAAGCATTTCAAGGGATGAC
AATAGCCGTACTTTATGGAGGCAGTTCTCAAAGATTTCAGATTGCACCGCTAGTGATCGCCACAACCCATCAGCTGATGA
GGTACAAAAATGCATTTGATGTGGTCATTGTAGATGAAGTCGATGCCTTTCCTTATTCAATGGATGAACGTCTCCAATTT
GCTGTTCTAAAGGCGATGAGAAAGAACGGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAAAATGACGAGAGA
GGTTTCTAGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCATAAACAACCATTACCCGTACCAACCTTTCATT
GGATTGGACATTGGAAAAAGAAGTTAAAAAAGAATCAGCTGCCCCGTAAAGTGATGAATTGGATGCAGAGACATATTGCA
AATAAAAGAAGAGTATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCGAGAACACTCCTT
AAACGTGGAGGGAGTATCTGCTGATGATCCAGATAGGAAACAAAAGGTTCAACACTTTAGAGATTACGTATACGATGTAC
TAGTTACAACCACTATTCTAGAAAGAGGCGTAACCATTCCAAATGTTCAAGTGGGGGTATTAGGTTCGGAATCTACTATT
TTTACAGAGAGTGCACTTGTTCAGATTTCTGGAAGAGTAGGAAGACACCCGGATTATTGTACAGGAGACGTTTTCCTTTT
TCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGTTAAAATGAATGATACGGCTGCAAATGAGTTTT
CTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(239-336)

Type III restriction enzyme, res subunit (ResIII)

(32-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

56.216

97.113

0.546
  comFA/cflA Streptococcus pneumoniae Rx1

41.618

90.814

0.378
  comFA/cflA Streptococcus pneumoniae D39

41.618

90.814

0.378
  comFA/cflA Streptococcus pneumoniae R6

41.618

90.814

0.378
  comFA Latilactobacillus sakei subsp. sakei 23K

39.071

96.063

0.375
  comFA/cflA Streptococcus pneumoniae TIGR4

41.329

90.814

0.375
  comFA/cflA Streptococcus mitis SK321

41.04

90.814

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

41.04

90.814

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

38.333

94.488

0.362

Multiple sequence alignment