Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHI58_RS17065 Genome accession   NZ_CP152035
Coordinates   3289709..3290836 (-) Length   375 a.a.
NCBI ID   WP_045033977.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2869     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284709..3295836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI58_RS17030 (MHI58_17030) flgL 3284892..3285803 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  MHI58_RS17035 (MHI58_17035) flgK 3285812..3287332 (-) 1521 WP_024719239.1 flagellar hook-associated protein FlgK -
  MHI58_RS17040 (MHI58_17040) - 3287348..3287830 (-) 483 WP_039169793.1 flagellar protein FlgN -
  MHI58_RS17045 (MHI58_17045) flgM 3287849..3288112 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHI58_RS17050 (MHI58_17050) - 3288192..3288611 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  MHI58_RS17055 (MHI58_17055) comFC 3288668..3289354 (-) 687 WP_176544677.1 ComF family protein Machinery gene
  MHI58_RS17060 (MHI58_17060) - 3289351..3289644 (-) 294 WP_008348267.1 competence protein ComFB -
  MHI58_RS17065 (MHI58_17065) comFA 3289709..3290836 (-) 1128 WP_045033977.1 DEAD/DEAH box helicase Machinery gene
  MHI58_RS17070 (MHI58_17070) - 3291208..3292050 (-) 843 WP_024719242.1 DegV family protein -
  MHI58_RS17075 (MHI58_17075) degU 3292262..3292951 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHI58_RS17080 (MHI58_17080) degS 3293016..3294191 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  MHI58_RS17085 (MHI58_17085) - 3294413..3295081 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42962.01 Da        Isoelectric Point: 10.4855

>NTDB_id=986089 MHI58_RS17065 WP_045033977.1 3289709..3290836(-) (comFA) [Bacillus sp. FSL K6-2869]
MLGKVSECGFLYEWTGPQKKETRRVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQYFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPVF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=986089 MHI58_RS17065 WP_045033977.1 3289709..3290836(-) (comFA) [Bacillus sp. FSL K6-2869]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACGAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTGGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGGTTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAAGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGTATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTGTTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTATTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTGAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.405

98.667

0.547
  comFA/cflA Streptococcus pneumoniae Rx1

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae D39

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae R6

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.859

98.133

0.381
  comFA/cflA Streptococcus pneumoniae TIGR4

40.395

94.4

0.381
  comFA/cflA Streptococcus mitis NCTC 12261

40.113

94.4

0.379
  comFA/cflA Streptococcus mitis SK321

38.983

94.4

0.368
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365

Multiple sequence alignment