Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R8559_RS10045 Genome accession   NZ_AP026926
Coordinates   1920582..1921043 (-) Length   153 a.a.
NCBI ID   WP_000250548.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700119     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1915582..1926043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8559_RS10010 (PC0116_19540) - 1915893..1916717 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  R8559_RS10015 (PC0116_19550) rnpA 1916692..1917063 (-) 372 WP_000739246.1 ribonuclease P protein component -
  R8559_RS10020 - 1917080..1917211 (-) 132 WP_000768903.1 hypothetical protein -
  R8559_RS10025 (PC0116_19560) - 1917212..1918402 (-) 1191 WP_000167759.1 acetate kinase -
  R8559_RS10030 (PC0116_19570) comYH 1918453..1919406 (-) 954 WP_000345123.1 class I SAM-dependent methyltransferase Machinery gene
  R8559_RS10035 (PC0116_19580) - 1919467..1920054 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R8559_RS10040 (PC0116_19590) comGG/cglG 1920191..1920604 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8559_RS10045 (PC0116_19600) comGF/cglF 1920582..1921043 (-) 462 WP_000250548.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8559_RS10050 (PC0116_19610) comGE/cglE 1921006..1921308 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8559_RS10055 (PC0116_19620) comGD/cglD 1921271..1921675 (-) 405 WP_000588007.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8559_RS10060 (PC0116_19630) comGC/cglC 1921668..1921934 (-) 267 WP_050123149.1 competence type IV pilus major pilin ComGC Machinery gene
  R8559_RS10065 (PC0116_19640) - 1921936..1922193 (-) 258 WP_000698513.1 hypothetical protein -
  R8559_RS10070 (PC0116_19650) - 1922463..1923419 (-) 957 WP_317639174.1 N-acetylmuramoyl-L-alanine amidase family protein -
  R8559_RS10075 (PC0116_19660) - 1923422..1923757 (-) 336 WP_050200954.1 phage holin -
  R8559_RS10080 (PC0116_19670) - 1923761..1924060 (-) 300 WP_001811580.1 hypothetical protein -
  R8559_RS10085 (PC0116_19680) - 1924069..1924419 (-) 351 WP_221014204.1 hypothetical protein -
  R8559_RS10090 (PC0116_19690) - 1924422..1924625 (-) 204 WP_001091118.1 hypothetical protein -
  R8559_RS10095 - 1924606..1924722 (-) 117 WP_001063633.1 hypothetical protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17851.34 Da        Isoelectric Point: 6.4832

>NTDB_id=98459 R8559_RS10045 WP_000250548.1 1920582..1921043(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700119]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=98459 R8559_RS10045 WP_000250548.1 1920582..1921043(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700119]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAAGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis SK321

92.157

100

0.922
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909
  comYF Streptococcus mutans UA159

41.791

87.582

0.366
  comYF Streptococcus mutans UA140

41.791

87.582

0.366

Multiple sequence alignment