Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NSQ70_RS16260 Genome accession   NZ_CP151992
Coordinates   3156193..3157320 (-) Length   375 a.a.
NCBI ID   WP_251185086.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0397     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3151193..3162320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ70_RS16225 (NSQ70_16225) flgL 3151376..3152287 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  NSQ70_RS16230 (NSQ70_16230) flgK 3152296..3153816 (-) 1521 WP_058336981.1 flagellar hook-associated protein FlgK -
  NSQ70_RS16235 (NSQ70_16235) - 3153832..3154314 (-) 483 WP_113766912.1 flagellar protein FlgN -
  NSQ70_RS16240 (NSQ70_16240) flgM 3154333..3154596 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NSQ70_RS16245 (NSQ70_16245) - 3154676..3155095 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NSQ70_RS16250 (NSQ70_16250) comFC 3155152..3155733 (-) 582 WP_342502276.1 ComF family protein Machinery gene
  NSQ70_RS16255 (NSQ70_16255) - 3155835..3156128 (-) 294 WP_008348267.1 competence protein ComFB -
  NSQ70_RS16260 (NSQ70_16260) comFA 3156193..3157320 (-) 1128 WP_251185086.1 DEAD/DEAH box helicase Machinery gene
  NSQ70_RS16265 (NSQ70_16265) - 3157692..3158534 (-) 843 WP_024719242.1 DegV family protein -
  NSQ70_RS16270 (NSQ70_16270) degU 3158746..3159435 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NSQ70_RS16275 (NSQ70_16275) degS 3159500..3160675 (-) 1176 WP_008360205.1 sensor histidine kinase Regulator
  NSQ70_RS16280 (NSQ70_16280) - 3160897..3161565 (+) 669 WP_251185087.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42893.89 Da        Isoelectric Point: 10.4727

>NTDB_id=983946 NSQ70_RS16260 WP_251185086.1 3156193..3157320(-) (comFA) [Bacillus sp. FSL R5-0397]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=983946 NSQ70_RS16260 WP_251185086.1 3156193..3157320(-) (comFA) [Bacillus sp. FSL R5-0397]
ATGCTTGGAAAAGTAAGTGAATGTGGCTTTCTCTATGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAACTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCATTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTTTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACGATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

56.216

98.667

0.555
  comFA/cflA Streptococcus pneumoniae Rx1

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae D39

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae R6

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae TIGR4

40.96

94.4

0.387
  comFA/cflA Streptococcus mitis NCTC 12261

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis SK321

39.548

94.4

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368

Multiple sequence alignment