Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NST24_RS16840 Genome accession   NZ_CP151977
Coordinates   3248247..3249374 (-) Length   375 a.a.
NCBI ID   WP_342462019.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0286     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243247..3254374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST24_RS16805 (NST24_16805) flgL 3243430..3244341 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  NST24_RS16810 (NST24_16810) flgK 3244350..3245870 (-) 1521 WP_058336981.1 flagellar hook-associated protein FlgK -
  NST24_RS16815 (NST24_16815) - 3245886..3246368 (-) 483 WP_007499977.1 flagellar protein FlgN -
  NST24_RS16820 (NST24_16820) flgM 3246387..3246650 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NST24_RS16825 (NST24_16825) - 3246730..3247149 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NST24_RS16830 (NST24_16830) comFC 3247206..3247892 (-) 687 WP_172793290.1 ComF family protein Machinery gene
  NST24_RS16835 (NST24_16835) - 3247889..3248182 (-) 294 WP_008348267.1 competence protein ComFB -
  NST24_RS16840 (NST24_16840) comFA 3248247..3249374 (-) 1128 WP_342462019.1 DEAD/DEAH box helicase Machinery gene
  NST24_RS16845 (NST24_16845) - 3249746..3250588 (-) 843 WP_024719242.1 DegV family protein -
  NST24_RS16850 (NST24_16850) degU 3250800..3251489 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NST24_RS16855 (NST24_16855) degS 3251554..3252729 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  NST24_RS16860 (NST24_16860) - 3252951..3253619 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42890.85 Da        Isoelectric Point: 10.4270

>NTDB_id=983052 NST24_RS16840 WP_342462019.1 3248247..3249374(-) (comFA) [Bacillus sp. FSL R5-0286]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVTHRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=983052 NST24_RS16840 WP_342462019.1 3248247..3249374(-) (comFA) [Bacillus sp. FSL R5-0286]
ATGCTGGGAAAAGTAAGTGAATGTGGCTTTCTCTATGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTAGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTACTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTCGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTGATGA
GATATAAAAACGCCTTTGATGTCCTTATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAGTCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAAGATGT
TACTCATAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTATACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae D39

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae R6

40.96

94.4

0.387
  comFA/cflA Streptococcus pneumoniae TIGR4

40.678

94.4

0.384
  comFA/cflA Streptococcus mitis NCTC 12261

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

38.315

98.133

0.376
  comFA/cflA Streptococcus mitis SK321

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365

Multiple sequence alignment