Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R8625_RS09580 Genome accession   NZ_AP026923
Coordinates   1847294..1847755 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae strain PZ900700054     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1842294..1852755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8625_RS09545 (PC0054_18570) - 1842599..1843423 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  R8625_RS09550 (PC0054_18580) rnpA 1843398..1843769 (-) 372 WP_000739246.1 ribonuclease P protein component -
  R8625_RS09555 - 1843786..1843917 (-) 132 WP_000768904.1 hypothetical protein -
  R8625_RS09560 (PC0054_18590) - 1843918..1845108 (-) 1191 WP_000167769.1 acetate kinase -
  R8625_RS09565 (PC0054_18600) comYH 1845159..1846112 (-) 954 WP_317649157.1 class I SAM-dependent methyltransferase Machinery gene
  R8625_RS09570 - 1846173..1846767 (-) 595 Protein_1857 class I SAM-dependent methyltransferase -
  R8625_RS09575 (PC0054_18630) comGG/cglG 1846870..1847316 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8625_RS09580 (PC0054_18640) comGF/cglF 1847294..1847755 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8625_RS09585 (PC0054_18650) comGE/cglE 1847718..1848020 (-) 303 WP_317649158.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8625_RS09590 (PC0054_18660) comGD/cglD 1847983..1848417 (-) 435 WP_000608686.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8625_RS09595 (PC0054_18670) comGC/cglC 1848380..1848643 (-) 264 WP_000962025.1 competence type IV pilus major pilin ComGC Machinery gene
  R8625_RS09600 (PC0054_18680) - 1848645..1848902 (-) 258 WP_000698511.1 hypothetical protein -
  R8625_RS09605 (PC0054_18690) - 1849173..1850129 (-) 957 WP_219601358.1 N-acetylmuramoyl-L-alanine amidase family protein -
  R8625_RS09610 (PC0054_18700) - 1850133..1850465 (-) 333 WP_050085269.1 phage holin -
  R8625_RS09615 (PC0054_18710) - 1850469..1850768 (-) 300 WP_001811580.1 hypothetical protein -
  R8625_RS09620 (PC0054_18720) - 1850777..1851127 (-) 351 WP_000852245.1 hypothetical protein -
  R8625_RS09625 (PC0054_18730) - 1851130..1851333 (-) 204 WP_001091109.1 hypothetical protein -
  R8625_RS09630 - 1851314..1851430 (-) 117 WP_001063633.1 hypothetical protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=98217 R8625_RS09580 WP_000250523.1 1847294..1847755(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700054]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=98217 R8625_RS09580 WP_000250523.1 1847294..1847755(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700054]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment