Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACNJZ5_RS05170 Genome accession   NZ_CP182371
Coordinates   1116271..1117509 (+) Length   412 a.a.
NCBI ID   WP_019842344.1    Uniprot ID   -
Organism   Escherichia coli strain Br_112_kylling     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1111271..1122509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNJZ5_RS05145 mrcA 1111551..1114103 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  ACNJZ5_RS05150 hofM 1114223..1115002 (+) 780 WP_001551759.1 DNA utilization protein HofM -
  ACNJZ5_RS05155 hofN 1115002..1115541 (+) 540 WP_001069326.1 DNA utilization protein HofN -
  ACNJZ5_RS05160 hofO 1115525..1115965 (+) 441 WP_001551756.1 DNA utilization protein HofO -
  ACNJZ5_RS05165 hofP 1115955..1116359 (+) 405 WP_001551755.1 DNA utilization protein HofP -
  ACNJZ5_RS05170 comE 1116271..1117509 (+) 1239 WP_019842344.1 DNA uptake porin HofQ Machinery gene
  ACNJZ5_RS05175 aroK 1117911..1118432 (+) 522 WP_000818618.1 shikimate kinase AroK -
  ACNJZ5_RS05180 aroB 1118489..1119577 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  ACNJZ5_RS05185 damX 1119669..1120955 (+) 1287 WP_001551753.1 cell division protein DamX -
  ACNJZ5_RS05190 dam 1121062..1121898 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44694.18 Da        Isoelectric Point: 5.9488

>NTDB_id=980552 ACNJZ5_RS05170 WP_019842344.1 1116271..1117509(+) (comE) [Escherichia coli strain Br_112_kylling]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=980552 ACNJZ5_RS05170 WP_019842344.1 1116271..1117509(+) (comE) [Escherichia coli strain Br_112_kylling]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACGCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362

Multiple sequence alignment