Detailed information NTG0979974

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AAFA25_RS02360
• Genome accession: NZ_CP151694
• Coordinates: 494904..495962 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain F19-10-CH3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 489904..500962

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA25_RS02345 (AAFA25_02345)	-	491010..491342 (+)	333	WP_000169599.1	hypothetical protein	-
AAFA25_RS02350 (AAFA25_02350)	-	491345..492169 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
AAFA25_RS02355 (AAFA25_02355)	ponA	492187..494742 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
AAFA25_RS02360 (AAFA25_02360)	pilM	494904..495962 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
AAFA25_RS02365 (AAFA25_02365)	pilN	495962..496603 (+)	642	WP_000201221.1	PilN domain-containing protein	Machinery gene
AAFA25_RS02370 (AAFA25_02370)	pilO	496600..497340 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
AAFA25_RS02375 (AAFA25_02375)	pilP	497351..497878 (+)	528	WP_000695061.1	pilus assembly protein PilP	Machinery gene
AAFA25_RS02380 (AAFA25_02380)	pilQ	497941..500106 (+)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
AAFA25_RS02385 (AAFA25_02385)	aroK	500118..500687 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=979974 AAFA25_RS02360 WP_002014066.1 494904..495962(+) (pilM) [Acinetobacter baumannii strain F19-10-CH3]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=979974 AAFA25_RS02360 WP_002014066.1 494904..495962(+) (pilM) [Acinetobacter baumannii strain F19-10-CH3]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423